Method for measuring content of pinoresinol diglucoside in eucommia ulmoides medicinal material

Abstract

The invention discloses a method for measuring the content of pinoresinol diglucoside in eucommia ulmoides medicinal materials. The method comprises the following steps: Step 1): preparing a referencesolution of pinoresinol diglucoside by using a methanol solution as a solvent; Step 2): processing eucommia ulmoides medicinal materials into floccules to obtain a sample to be detected; Step 3): sequentially extracting the sample to be detected by chloroform and methanol, and dissolving the sample with methanol to obtain a test solution; Step 4): measuring the content of pinoresinol diglucosidein the reference solution and the test solution by high performance liquid chromatography; the chromatographic detection conditions are as follows: octadecylsilane chemically bonded silica is used asa filler; the volume ratio of a mobile phase is 25:75 methanol-water solution; the detection wavelength is 277 nm; the sample size is 10 uL; and the technical scheme adopted by the invention solves the problems that the spectrogram obtained by the eucommia ulmoides detection method has poor chromatographic peak performance and more miscellaneous peaks when the eucommia ulmoides content is determined in the prior art.

Description

technical field [0001] The invention relates to the field of content detection of pharmaceutical ingredients, in particular to a method for determining the content of pinoresinol diglucoside in eucommia medicinal materials. Background technique [0002] Eucommia ulmoides is the dry bark of Eucommia ulmoides, also known as Sixian, Sizhong, and kapok. It is a precious nourishing medicinal material in my country. As a traditional Chinese medicine, Eucommia has a history of more than 2,000 years of medicinal use in my country. It has many functions such as nourishing the liver and kidney, strengthening bones and muscles, lowering blood pressure, anti-tumor, and preventing miscarriage. "Shen Nong's Herbal Classic" listed it as a top grade. At present, it is known that the main chemical components of Eucommia include dozens of compounds such as lignin, iridoids, phenylpropanoids, flavonoids, etc. Studies have shown that some of the active components are aucubin, pinoresinol digluc...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of assay method of pinoresinol diglucoside content in the Eucommia medicinal material, solves the problem that the chromatographic peak type all exists in the spectrogram obtained by the Eucommia detection method when measuring the Eucommia content at present, and the sample processing time is long, Problems such as too many peaks

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