74 results about "Aucubin" patented technology

The invention discloses a method for extracting active sites of iridoid, and extracting monomer geniposidic acid (GPA), geniposide(GP) and aucubin(AU) from eucommia, wherein raw materials are extracted by taking the eucommia(husk, leaf or seed) as materials and utilizing the conventional leaching method, the ultrasonic extraction method or the microwave extraction method, and an extracting solution is preliminarily purified by methods such as the solvent extraction and the adsorption separation of special adsorbents, etc., and thus the active sites of the iridoid(the total content of the iridoid is no less than 50 percent) is obtained, and GPA, GP and AU undergo the separation, low temperature condensation, refrigeration and drying by using the preparation high pass filter technique and taking an alcohol-water solution as the mobile phase to obtain monomers of GPA, GP and AU, contents of which are all no less than 90 percent. The preparation method disclosed by the invention utilizes novel process of the extraction and separation, and can simultaneously prepare a plurality of monomer products of the eucommia, and provides scientific foundations for the comprehensive development and utilization of the eucommia; moreover, the whole process does not use solvents harmful to human bodies, thereby realizing the green preparation.

The invention belongs to the technical field of medicines and relates to a method for preparing high-purity aucubin. In the method, normal hexane is degreased, and the residues are soaked in water or aqueous solution of ethanol for ultrasonic extraction; the obtained extract is passed through a chromatographic column of a polymer filler solid phase, the chromatographic column is eluted by a small amount of water and low-concentration aqueous solution of ethanol, the separated product is concentrated, the concentrate is separated by a silicagel column and recrystallization is performed to obtain the high-purity aucubin. The method has the advantages that: products can be produced in large scale and with high purity; the adopted polymer filler solid phase and the adopted silicagel column can be used repeatedly; the cost is low; the organic phase used for elution is free from organic solvents which have high toxicity and cause heavy pollution to environment; and the recovered organic phase can be used repeatedly.

The invention relates to a technological process for extracting aucubin from fruits of eucommia ulmoides oliver, which comprises the following steps: cutting the fruits of the eucommia ulmoides oliver into pieces, extracting with water or ethanol solution as an extraction solvent, and purifying the obtained extracting solution with macroporous resin to obtain the aucubin the purity of which is more than 50%. The technological process has the advantages of low toxicity, safety, short process time, no environmental pollution, simple and convenient operation, easy industrialization enlargement, high yield and good purity of the product, thus meeting various needs for development of drugs taking the fruits of the eucommia ulmoides oliver as main raw materials.

The invention relates to a method for extracting aucubin from aucuba japonica leaves, which comprises the following steps of taking the aucuba japonica leaves as a raw material; extracting for 2 to 3 times by utilizing ethanol in a microwave mode; recovering the ethanol from an extracting solution; adding water in extract to be dispersed and filtered; adding a polarity macroporous resin column to absorb; eluting with 50 to 70% of the ethanol; ultra-filtering eluent by utilizing an ultra-filtration membrane; concentrating by utilizing a nano-filtration membrane; extracting a concentrated solution by adding ethyl acetate; concentrating and crystallizing the extracting solution; and washing crystals with chloroform and petroleum ether and drying in a low temperature and vacuum conditions. The process of the invention has the advantages of simple operation, low energy consumption, high extraction efficiency, the obtained product has high content, and the temperature in the process of production is easy to control. The method of the invention is easy to product in industrialization.

The invention belongs to the technical field of medicine and in particular relates to a method for extracting aucubin from eucommia ulmoides oliver seed draff. The method comprises the following steps: carrying out flash type extraction or ultrasonic extraction on crude eucommia ulmoides oliver seed draff powder; removing an extraction solvent and water-insoluble substances in extract; based on a water layer extracted by an organic solvent for an aqueous solution as a sampling liquid, processing the sampling liquid by virtue of a macroporous absorption resin column, and successively eluting with water and ethanol; collecting alcohol eluent, then concentrating, and drying so as to obtain the aucubin. According to the invention, the raw material cost is cheap; the method has the advantages of fast extraction speed, short period, low energy consumption and no pollution and is simple to operate; the product is high in yield and stable in quality; and the method is easy for industrial production.

The invention provides a method for rapidly and efficiently preparing an aucubin monomer from eucommia ulmoides. The method disclosed by the invention comprises the following steps of: taking eucommia ulmoides leaves / peels / seed cakes as raw materials; adding methanol or ethyl acetate according to a certain ratio to carry out ultrasonic reflowing and extraction; filtering; decompressing a filtering solution and recycling a solvent to a suitable amount; after cooling, separating light brown sediment; adding suitable amounts of ethyl acetate-methanol; condensing and reflowing; hotly filtering; standing at a room temperature; and separating sediments and filtering to obtain a white grain crystal of aucubin. The method disclosed by the invention adopts abundant raw material resources and the aucubin monomer can be prepared by two steps of extracting and crystallizing; and the method has an environment-friendly and simple process, is low in equipment requirements and can easily realize industrialized production. The industrial production of the aucubin enhances development and utilization values of the eucommia ulmoides in fields of medical treatment and health care, and brings about economic and social benefits.

The invention discloses a method for extraction and separation of chlorogenic acid, pinoresinol diglucoside, aucubin and gutta-percha from a eucommia ulmodies oliv raw material. The method comprises the steps: extracting from the eucommia ulmodies oliv raw material by an organic solvent, concentrating, successively carrying out hot water dissolving, cold precipitation and centrifugal separation on the concentrate to obtain a centrifugal liquid, carrying out enrichment, elution and concentration on the centrifugal liquid, to obtain an eucommia ulmodies oliv extract, separating and purifying the eucommia ulmodies oliv extract, and thus respectively obtaining the pinoresinol diglucoside, chlorogenic acid and aucubin; and carrying out hydrolysis and organic solvent extraction on the extract residue, to obtain the gutta-percha. The method extracts and separates the high-purity chlorogenic acid, pinoresinol diglucoside, aucubin and gutta-percha from the eucommia ulmodies oliv with relatively high yield simultaneously, has the advantages of simple operation and low cost, realizes the comprehensive utilization of natural resources, and meets industrialized production.

The invention discloses a new application of a chemical component of chemical component used as plant estrogen, in particular relates to an application of an eucommia bark extract in preparing a medicine for treating and / or preventing a disease relevant to the estrogen hyposecretion of a mammal (particularly a human). The invention relates to an application of a composition in preparing a medicine for treating and / or preventing a disease relevant to the estrogen hyposecretion of a mammal (particularly a human), which is selected from one or more of the following components: betulinic acid, genipin, aucubin, pinoresinol diglucoside, syringaresinol diglucoside, abietinol monoglucoside, syringaresinol monoglucoside, wogonin, oroxylin A, baicalein and dihydrochalcone 3-O-beta-D-glucoside and a medicine composition containing the eucommia bark extract or an active component in the eucommia bark extract. The eucommia bark extract and a monomer chemical component in the eucommia bark extracthave the effect of effective plant estrogen.

The invention relates to an extraction method of aucubin and an application of the aucubin. The method comprises the simple steps of crushing plantain, soaking in alcohol overnight, conducting ultrasonic extraction, merging, recovering, concentrating and centrifuging an extracting solution, obtaining supernate, adopting macroporous resin and eluting an alcohol solution, conducting thin-layer chromatography detection, collecting and concentrating fraction at a target point, conducting silicagel column chromatography on a concentrate, and then collecting and concentrating the fraction at the target point, repeating the silicagel column chromatography to obtain macrocrystalline, purifying a crude product through high performance liquid chromatography, collecting target components, freezing and drying, and finally obtaining white amorphous powder. The substance extracted from the plantain by the method is the aucubin. The aucubin can be used for treating and preventing diabetes mellitus and concurrent encephalopathy of the diabetes mellitus, the occurrence and development mechanisms of the diabetes mellitus and complications of the diabetes mellitus are further deeply studied, and new-generation drugs for resisting the diabetes mellitus and the complications of the diabetes mellitus can be developed.

The present invention provides a method for producing aucubin used as medicinal ingredient by the induction of Eucommia fairy root, which obtains the Eucommia fairy root by genetic transformation for the production of the aucubin. The method includes the following processes: Agrobacterium rhizogenes are used to genetically transform Eucommia ulmoides Oliv. in order to obtain the Eucommia fairy root, which is determined as the genetically transformed fairy root by molecular detection, and HPLC detection indicates that the Eucommia fairy root obtained by genetic transformation can produce the aucubin. The method can provide novel sustainable medicine source for the production of the aucubin used as medicinal ingredient.

The invention discloses a method for separating and purifying aucubin from leaves of eucommia ulmoides oliv with an NKA-2 macroporous adsorption resin, which comprises the following steps: extracting the crude extract of aucubin from leaves of eucommia ulmoides oliv with neutral eatable alcohol, and separating and purifying the crude extract of aucubin with the NKA-2 macroporous adsorption resin to obtain an aucubin extract. According to the method, the aucubin is extracted and separated with the eatable alcohol safely and non-toxically, so that not only aucubin extracts of different purities can be industrially produced safely and efficiently, but also the safe, convenient, rational and economic high-value utilization of eucommia ulmoides oliv resources and the industrial production of a series of products are implemented, and furthermore, an environment-friendly chemical production idea and an economy circulation policy are fully reflected.

The invention discloses an aucubin-enriched eucommia seed oil soft capsule that is rich in alpha-linolenic acid and aucubin and a preparation method thereof. The prepared aucubin-enriched eucommia seed oil soft capsule consists of eucommia seed oil and residues powder of the eucommia seed oil according to the weight percentage of 7:3 or 6:4, wherein, the aucubin-enriched eucommia seed oil soft capsule contains 3%-4% of the aucubin. The preparation method comprises seven steps of picking seeds, cleaning and selecting, leaching, shelling, pressing, burdening and packing in soft capsule. The aucubin-enriched eucommia seed oil soft capsule prepared by the invention not only can bring the composite synergized action of the alpha-linolenic acid and the aucubin into play completely, but also uses the spent material (residues) completely after extracting eucommia seed oil, thus developing and using eucommia kernel comprehensively, and the invention has simple technology, is easy for operating and has good market prospect.

Disclosed are a eucommia leaf extract, and preparation method and use thereof. The extract is a liquid of the eucommia leaf or an ethyl alcohol extract, and has chlorogenic acid of a content not less than 9%, geniposidic acid of a content not less than 1% and aucubin of a content not less than 1%. The extract has a good effect on psoriasis.

The invention discloses a method for extracting aucubin from folium cortex eucommiae, aucubin powder is prepared by crushing of the folium cortex eucommiae, cellulase assisted extraction in medium temperature water, conventional filtration, impurity removal by a ultrafiltration membrane, nanofiltration concentration, separation and purification by macroporous resin, concentration and drying; according to the method, the aucubin is extracted from the folium cortex eucommiae by cellulase assisted extraction in the medium temperature water, the extraction rate can reach 95%, the water extraction temperature is lower, and the extraction time shorter; the ultrafiltration membrane is used for impurity removal of an extracting solution, and separation of other by-products, the membrane operation process is simple, the membrane flux can be stable for a long time, the membrane is easy to clean and long in service life and realizes industrialization; an nanofiltration membrane is used for concentration of the impurity removed extracting solution, the load of follow-up processes can be reduced, and the service life of resin is lengthened; the COD value of a nanofiltration filtrate is less than or equal to 100, the nanofiltration filtrate can be reused in the production process, and basically no waste water is not discharged; the macroporous adsorption resin can effectively purify the aucubin, purity of the aucubin in the prepared powder is greater than 62%, and the extraction rate is more than 2.8%.

The invention belongs to the field of extraction of traditional Chinese medicine, and particularly relates to a cortex eucommiae extract composition for treating renal fibrosis and application of thecortex eucommiae extract composition. The cortex eucommiae extract composition is prepared from the components of aucubin, a mixed extract of geniposide and geniposidic acid, quercetin and chlorogenicacid through the steps of extraction, separation, purification and the like, and the cortex eucommiae extract composition can be further prepared into a preparation. A preparation method is simple and efficient, and the prepared cortex eucommiae extract composition has the good effect of treating renal fibrosis.

The invention discloses a Eucommia ulmoides rubber refining method. The method comprises the following steps: 1, preprocessing Eucommia ulmoides leaves, and sequentially removing chlorophyll, beta-carotene, lignans, aucubins, chlorogenic acid, proteins, flavones and polysaccharide compounds from the Eucommia ulmoides leaves; 2, extracting Eucommia ulmoides rubber by adopting petroleum ether; and 3, adding a precipitating agent to a petroleum ether extract liquid obtained in step 2, and allowing the obtained mixture to stand for precipitation to obtain the Eucommia ulmoides rubber. The Eucommia ulmoides rubber obtained by refining through the method has a light color, solves a problem that the export of the Eucommia ulmoides rubber is limited because of the dark color, and has the advantages of low extraction cost, almost zero cost of the raw material, less energy consumption, small solvent loss, and high circulating utilization rate. A new reference direction is provided for the industrialized production.

The invention discloses an application of aucubin or aglycone thereof to preparation of a medicine for inhibiting ferroptosis, belongs to the field of an application of traditional Chinese medicine extracts, and particularly provides an application of aucubin or aglycone thereof to preparation of the medicine for inhibiting ferroptosis. The invention relates to an application of aucubin, in particular to an application of aucubin or aglycone thereof to preparation of the medicine for preventing or treating diseases related to ferroptosis. The inhibition effect and the molecular mechanism of the aucubin or the aglycone thereof on ferroptosis are disclosed for the first time, the pharmacological action basis of the aucubin or the aglycone thereof is enriched, and a new way is provided for prevention or treatment of diseases related to ferroptosis.

The invention discloses a preparation method of eucommia seed protein. The method comprises the following steps: (1) drying and crushing eucommia seeds to 20-80 meshes, putting the crushed eucommia seeds in a supercritical carbon dioxide extraction kettle, and removing grease in the eucommia seeds by supercritical carbon dioxide extraction to obtain eucommia seeds for later use; and (2) adding water in the eucommia seeds, regulating the pH value to 8-12, starting microwave to boil the solution for 3-10 minutes, centrifugally filtering and collecting filtrate, adding hydrochloric acid in the filtrate to regulate the pH value to 3-5 so as to precipitate protein, and carrying out freeze drying to obtain the eucommia seed protein. The preparation method disclosed by the invention has the advantages of high extraction efficiency, simple operation, high product yield and good storage of active ingredient aucubin, and the extraction rate of protein in the eucommia seeds is higher than 80%.

The invention discloses a folium cortex eucommiae and sweet potato mixed fermentation product and a preparation method thereof, the eucommia has the mutiple advantages that functional components such as chlorogenic acid and aucubin in the folium cortex eucommiae have the good effects on aspects of diuresis, antivirus and the like. According to the folium cortex eucommiae and sweet potato mixed fermentation product and the preparation method thereof provided by the invention, bacillus subtilis is adopted for saccharifying sweet potatoes, the saccharified sweet potatoes are fermented by fermented lactobacillus, and sweet potato starch pasting matter embedding mode is adopted to obtain the folium cortex eucommiae and sweet potato mixed fermentation product; the taste of a feedstuff is greatly improved by fermenting the bacillus subtilis and the lactobacillus, and meanwhile, the effective functional components such as chlorogenic acid in the feedstuff in the folium cortex eucommiae is farthest reserved; the method fully utilizes starch in the sweet potato, the starch is rapidly gelatinized in a high temperature process, a condition is provided for the embedding of folium cortex eucommiae, meanwhile, a sugar source is provided for lactobacillus by saccharifying, and the rich nutrients are provided for the feedstuff; the fragrance and the savour of the feedstuff are effectively improved through fermentation, meanwhile, active components such as chlorogenic acid do not influence the microbial growth, the disadvantage of the common savour of the traditional technology product is overcome, meanwhile, the fragrance of the product is also improved, and the sweet fragrance is rich and lasting.

The invention belongs to the technical field of medicine preparation, and particularly relates to a eucommia ulmoides extract composition and a preparation method and application thereof. The composition comprises the following components: geniposidic acid, chlorogenic acid, aucubin, and a mixture of pinoresinol diglucoside and dehydrodiconiferyl alcohol diglucoside. The extract composition can effectively treat primary hypertension or kidney deficiency syndrome, has no toxic or side effect, is small in dosage, and can effectively reduce the medication cost.

The invention provides a process for comprehensively extracting aucubin, chlorogenic acid and eucommea rubber from folium cortex eucommiae. The process comprises the following steps: extracting driedand ground folium cortex eucommiae with petroleum ether to obtain an extracting solution and filter residues; adding ethanol into the extracting solution to centrifugally take the lower solid, namelythe eucommea rubber, soaking and extracting the filter residues with ethanol, removing polysaccharides to obtain crude extract of aucubin and chlorogenic acid, and separating the crude extract of aucubin and chlorogenic acid by using a macroporous resin; finally, extracting with ethanol-ethyl acetate solutions respectively, passing through a polyamide column and a silica-gel column, and purifyingso as to obtain the final product. The process is simple in operation, low in production cost and high in yield, and can be used for large-scale production of the aucubin, chlorogenic acid and eucommea rubber industry.

The invention relates to the field of applications of traditional Chinese medicine extracts and in particular provides an application of aucubin to preparation of medicines for treating renal interstitial fibrosis. Aucubin is preferably the eucommia ulmoides samara extract, wherein the purity of aucubin is above 98%.

The invention discloses a preparation method of iridoid compounds, the iridoid compounds and application, and particularly relates to a preparation method of seven iridoid compounds and derivatives and application of the compounds and derivatives in inhibition of nitric oxide release activity. The preparation method comprises the following steps: carrying out ultrasonic extraction on degreased eucommia ulmoides seed meal by using pure methanol as a solvent, and carrying out chromatographic separation, recrystallization and other purification processes on an extracting solution to obtain the seven iridoid compounds including gram-grade aucubin. The degreased eucommia ulmoides seed meal is used as a raw material, so that the effective utilization of resources is realized. The operation is simple, convenient and effective, the product purity is high, and the requirements of development of various medicines taking eucommia ulmoides seeds as main raw materials can be met.

The invention discloses a pharmaceutical composition for treating tympanitis and a preparation method thereof. The pharmaceutical composition is prepared by taking herb of lepidium virginicum L., Chinese galls, honeycombs, herba taraxaci, rhizoma alismatis, herba periliae, aucubin and platycodin D as bulk pharmaceuticals in proportion and can be prepared into preparations of various forms according to a conventional preparation technology, and the tympanitis treating effect is significant.

The invention discloses a drug for potentially treating Parkinson's disease and belongs to the technical field of medical neuropharmacology. The invention finds the potential application of an iridoid glycoside compound aucubin in the drug for clinically treating Parkinson's disease. According to the invention, a Parkinson's disease animal model is established, various research methods, such as, behavioristics, immunology and biochemical criterion, are comprehensively utilized and an experiment proves that the drug can improve the dyskinesia symptom of a Parkinson's disease model rat; the decreasing of the content of corpus striatum dopamine is reduced; the dopaminergic neuron degeneration of the compact part of substantia nigra is reduced; the aucubin material source is abundant and the effect is excellent, so that the drug has a certain development value and prospect in clinical application.

The invention discloses a method for extracting aucubin from eucommia bark, and belongs to the technical field of aucubin extraction. The method includes the following steps: (1) performing coarse crushing on the fresh eucommia bark, putting the crushed eucommia bark into a sieve basket, immersing the whole basket into liquid nitrogen, and performing repeating for 2 to 3 times in the way; (2) drying the eucommia bark in the step (1) at low temperature, and performing crushing to obtain eucommia bark powder; (3) performing extraction on the eucommia bark powder by using a supercritical CO2 extraction process; and (4) allowing CO2 containing the extract to flow out from an extraction kettle to enter a separation kettle for decompression separation to obtain the eucommia bark aucubin. The method for extracting the aucubin from the eucommia bark provided by the invention avoids considering the loss of the aucubin during the extraction process of the eucommia bark, and improves the qualityand yield of the aucubin extracted from the eucommia bark.

The invention discloses a skincare no-clean hand-cleaning spray hand sanitizer and a preparation method. The skincare no-clean hand-cleaning spray hand sanitizer is prepared from the following raw materials in parts by weight: 200-300 parts of distilling water, 500-650 parts of absolute ethyl alcohol, 50-100 parts of biologically-refined glycerinum, 20-60 parts of sorbitol, 5-30 parts of eucommia ulmoides chlorogenic acid, 0.1-0.5 parts of aloetin, 0.1-0.5 parts of aloesin, 10-50 parts of flos chrysanthemi indici extracts, 0.01-0.05 parts of rose essential oil, 0.02-0.06 parts of salvia officinalis essential oil, 6-12 parts of aucubin and 5-15 parts of cactus extracts. The skincare no-clean hand-cleaning spray hand sanitizer is capable of realizing hand cleaning in a no-clean manner, and has effective sterilization and bacteriostasis effects; the selected additives are all plant extracts, which are mild, non-toxic and non-irritation; and meanwhile, the hand sanitizer also has the effects of skin whitening, anti-aging, and hand skin moisturizing.

The invention discloses a method of comprehensively extracting grease, proteins, aucubin, and eucommia ulmoides gum from eucommia seeds. The method comprises the following steps: taking eucommia seedsas the raw material; grinding eucommia seeds, adopting a double screw extrusion device to carry out extrusion expanding granulation; after granulation, adopting a roller press to carry out rolling, then carrying out squeezing to obtain eucommia oil and meal cakes; washing the meal cakes by alcohol to obtain washing liquid and residues; adsorbing the washing liquid by macroporous resin, then carrying out elution, vacuum concentration, and drying to obtain aucubin; extracting the residues by petroleum ether with a temperature of 60 to 90 DEG C to obtain extracts and residues; cooling the extracts, washing the extracts by acetone to obtain eucommia ulmoides gum; extracting the residues by a NaOH solution, and after extraction, performing acid precipitation, vacuum concentration, and drying to obtain eucommia seed proteins. Through abovementioned steps, four components in eucommia seeds namely grease, proteins, aucubin, and eucommia ulmoides gum are comprehensively extracted; the resources are saved, and the additional value is increased.

The invention discloses a capsule for subsequent filainous uveitis after cataract surgery. The capsule is mainly prepared from the following components: acetazolamide, taurine, aucubin, hyperoside, L-fucose, wogonin, water-soluble nutrient pigment, caffeic acid, beta-sitosterol, baicalein, pearl powder, ursolic acid, D-mannitol, vitamin A, vitamin C, diluent, lubricant, glidant, disintegrant and a wetting agent. According to the capsule, ocular circulatory disturbance can be improved, inflammatory response can be controlled, and retinal metabolism and immunoregulation functions can be enhanced, so that absorption of postoperative hyperplastic lesions can be promoted, inflammatory fibrous exudation can be inhibited, and vision recovery after cataract surgery can be promoted. The capsule has a remarkable curative effect, and is suitable for popularization.

The invention discloses a pharmaceutical composition for treating neuropathic pain, which is prepared by proportionally mixing crude drugs including morin, onjisaponin A, syringopicroside and aucubin. The pharmaceutical composition can be prepared in various forms according to conventional preparation technologies. The invention overcomes the defects in the prior art and provides the pharmaceutical composition which is effective and safe in treating neuropathic pain. The pharmaceutical composition provided by the invention is simple in preparation method and low in cost. Clinical tests prove that the pharmaceutical composition has remarkable clinical effects on treating neuropathic pain such as prosopalgia, ischialgia, radiculalgia, post-herpetic neuralgia and diabetic peripheral neuritis.