Preparation method of iridoid compounds, iridoid compounds and application

Abstract

The invention discloses a preparation method of iridoid compounds, the iridoid compounds and application, and particularly relates to a preparation method of seven iridoid compounds and derivatives and application of the compounds and derivatives in inhibition of nitric oxide release activity. The preparation method comprises the following steps: carrying out ultrasonic extraction on degreased eucommia ulmoides seed meal by using pure methanol as a solvent, and carrying out chromatographic separation, recrystallization and other purification processes on an extracting solution to obtain the seven iridoid compounds including gram-grade aucubin. The degreased eucommia ulmoides seed meal is used as a raw material, so that the effective utilization of resources is realized. The operation is simple, convenient and effective, the product purity is high, and the requirements of development of various medicines taking eucommia ulmoides seeds as main raw materials can be met.

Description

technical field [0001] The present invention relates to a method for extracting and separating plant active ingredients in the pharmaceutical industry, to a method for extracting iridoids from eucommia seed dregs and its anti-neurinflammation application, and to the preparation of seven iridoids Preparation method, especially the preparation method of iridoid compound, iridoid compound and application. Background technique [0002] Eucommia ulmoides Oliver (Eucommiaulmoides Oliver) is a tertiary relict plant that only exists in my country, and its bark is a traditional Chinese medicine. The chemical composition of Eucommia ulmoides is very rich. After research, it was found that the bark, leaves, seeds, branches, fruits and flowers of Eucommia ulmoides contain similar chemical components, mainly including lignans, iridoids, organic acids, flavonoids, polysaccharides, etc. Organic compounds such as sugars and Eucommia gum [1] . Eucommia ulmoides has the functions of nouris...

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Problems solved by technology

Existing patents or documents of Eucommia ulmoides seed meal have reports, such as Chinese patent (application number 200810031122.1) "a method for extracting iridoid active parts and monomers from eucommia ulmoides", which uses high-performance liquid chromatography Preparation, expensive equipment and limited production scale; Chinese patent (application number 201010297478.7) discloses "a process for extracting aucubin from Eucommia ulmoides", which mainly includes flash or ultrasonic extraction, macroporous resin adsorption purification, However, the purity of aucubin obtained by this process is only 50%, and the consumption of reagents is large, the operation is cumbersome, the industrial production cycle is long, and the cost is high; Chinese patent CN101863938 A discloses "a method for preparing high-purity aucubin Method", this method separates and obtains aucubin with polymer packing stationary phase chromatographic column, silica gel column chromatography, recrystallization (solvent is acetone and absolute ethanol), steps are numerous, and product yield is relatively low (in the embodiment The yields are respectively 0.955% and 2.5%), and the production cycle is longer; in the document New iridoidtrimers and tetramers from seeds of Eucommia ulmoides (Chem.Pharm.Bull.1990,38 (1) 267-269), water-containing methanol is used as solvent , four iridoid components (Ulmoidosides A, B, C, D) were isolated from Eucommia ulmoides seeds, but the specific preparation method did not involve

Most of these patents or documents are related to Eucommia ulmoides seed oil or only aucubin extraction, and there is no systematic research and development on the chemical components in eucommia ulmoides seed meal, and there are deficiencies in the extraction process

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