120 results about "Alternaria tomato" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention discloses a preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth. The bacillus amyloliquefaciens new strain SZ23 is preserved at China General Microbiological Culture Collection Center on July, 25th, 2014, and preservation number is CGMCC No.9481. Morphological characteristics, physiological and biochemical indexes, and 16S rDNA sequencing results of bacillus amyloliquefaciens new strain SZ23 are detailedly disclosed by the invention. It is shown by bacteriostasis experiments that, the fermentation broth of bacillus amyloliquefaciens new strain SZ23 possesses excellent bacteriostatic activity on alternaria kikuchiana, fusarium oxyspirum F sp niveum, fusarium oxysporum Schl.f.sp vasinfectum(Atk.)Snyd.& Hans, alternaria solani, fusarium wilt of cucumber, cotton rhizoctonia solani kuhn, fusarium graminearum schw, and botrytis cirerea Pers et Tris; bacteriostasis rate ranges from 55.6% to 92.2%; and it is confirmed that wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 possesses excellent broad-spectrum antibacterial activity. It is confirmed by practical experiments that control rate of the fermentation broth of new strain SZ23 on alternaria kikuchiana is more than 75%.

The invention relates to a strain of Bacillus subtilis as well its application. The bacterium is separated from the soil on lily fields, fanjiaping, Lanzhou, Gansu. The bacterium is identified to be Bacillus-subtilis. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus-subtilis M22BH3 and preserving code of M206028. The broth of M22BH3 and M22BH3 strain, non-cell culture fluid of M22BH3 strain's broth, and the monomer extracted from the M22BH3 strain, M22BH3 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention relates to citrus endophytic actinomycetes with antibacterial activity on various plant pathogens, belonging to the technical field of microbes. The endophytic actinomycetes are Streptomyces sp. R1020 which was registered and collected in China General Microbiological Culture Collection Center on March 17, 2014 with the collection number of CGMCC No. 8923. The strain R1020 has relatively good inhibitory effect on citrus anthrax bacteria, citrus bacterial canker disease bacteria, citrus penicillium italicum, citrus penicillium digitatum, bayberry deadwood disease bacteria, bayberry acicola, bayberry anthrax bacteria, loquat anthrax bacteria, loquat leaf spot fungi, loquat ringspot pathogenic bacteria, tomato alternaria solani, botrytis cinerea, colletotrichum orbiculare, tea tree anthrax bacteria and elsinoeleucospila and has the potential of being developed into biological pesticides.

The invention discloses bacillus velezensis ZF481 and application thereof. The strain number of the bacillus velezensis is ZF481, the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center on July 8, 2020, and the registration number of the bacillus velezensis is CGMCC No.20320. The bacillus velezensis ZF481 or / and the metabolite of the bacillus velezensis ZF481 can be used for preventing and / or treating the plant clubroot, or preparing a product for preventing and / or treating the plant clubroot. The strain has an inhibiting effect on the activity and germination of plasmodiophora brassicae resting spores, has a good growth promoting effect on brassicaceous crops such as Chinese cabbage and the like, and also has a good antibacterial effect on phytophthora capsici, stemphylium solani, fusarium oxysporum, alternaria solani, corynespora, Botryis cinerea, rhizoctonia solani and the like.

The invention relates to a botanical essential oil for the control of plant pathogenic fungus and a preparation method thereof. The essential oil of the invention is extracted from star aniseseen fruits and contains 29 main ingredients through gas chromatography-mass spectrometry analysis, wherein, trans-anethole accounts for 83.483 percent. The invention first confirms that the trans-anethole is the main antibacterial active ingredient in the essential oil from the star aniseseen as well as the essential oil from the star aniseseen and the trans-anethole thereof has significant inhibition activity for a plurality of plant pathogenic fungus such as early blight of tomato, stem end rot of mango, corn leaf blight, wheat scab, cucumber fusarium wilt, tomato fusarium wilt, cotton fusarium wilt, rice blast fungus, pythium aphani-dermatum, wheat sheath blight, rice sheath blight, etc. and can control plant diseases.

The invention relates to a Bacillus amyloliquefaciens B-004. The strain was preserved in China General Microbiological Culture Collection Center on January 17th, 2011, and the preservation number is CGMCC NO.4557. The strain has the obvious antagonistic effect on postharvest tomato early blight bacteria, can inhibit growth of tomato early blight bacteria, and can be further prepared into an agricultural bactericide using the strain as the effective ingredient, thereby having active and practical meanings for controlling postharvest tomato early blight in a biological control way.

The invention discloses a novel harmaline amide compound, a preparation method of the compound and an application of the compound in preparation of pesticides. The structural formula of the harmaline amide compound is shown as a formula (I); the structural formula is as shown in the specification, wherein in the formula (I), R1 is selected from hydrogen, methyl, phenyl, 2,4,5-trimethoxyphenyl, p-methoxyphenyl, p-trifluoromethylphenyl or p-chlorophenyl; R2 is selected from hydrogen or bromine; R3 is selected from hydrogen or ethyl; and R4 is selected from ethyl, 2-pyridyl, o-chlorophenyl, 4,6-dimethylpyrimidyl or p-trifluoromethylphenyl. The compound has good inhibitory activities on rhizoctonia solani, collectotrichum musae, grey mould germ of strawberry, fusarium oxyspirum F sp niveum and alternaria solani and has good application prospects in control of important agricultural pests. In addition, the artificial synthesis process is simple, the product purity is high, and the compound is suitable for large-scale industrial application.

The invention discloses aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and an application of aspergillus tubingensis metabolites. The bacterial strain No. of aspergillus tubingensis is QF05, and the registration No. of aspergillus tubingensis in CGMCC (China general microbiological culture collection center) is CGMCC No.13563. Aspergillus tubingensis QF05 has an inhibition function on botrytis cinerea, colletotrichum capsici, fusarium wilt of cucumbers, alternaria solani and pseudomonas syringae pv.lachrymans. Sterile fermentation filtrate of aspergillus tubingensis QF05 can remarkably increase the germination rate of pepper seeds and promote elongation of radicle. Aspergillus tubingensis QF05 metabolites containing bacteria effectively promote growth of true leaves of cucumbers and effectively increase the plant height and the yield of the cucumbers.

The invention relates to a polygonum aviculare botanical bactericide and a preparation method thereof, belonging to the technical field of pesticides. The invention aims at disclosing a compound botanical bactericide formula and a preparation method thereof. The polygonum aviculare botanical bactericide is mainly prepared from polygonum aviculare, radix scutellariae, black nightshade herb, radix gentianae, rhizoma atractylodis and the like. The preparation method comprises the steps of 1, drying and crushing the raw materials, then sieving and mixing the raw materials; 2, performing ultrasonic treatment on the mixture after being subjected to cold soak in ethanol, filtering to obtain a filtered liquid, evaporating and condensing until the filtered liquid becomes paste. The polygonum aviculare botanical bactericide has a relatively good bacteriostasis function on multiple pathogenic fungal plant pathogens such as rhizoctonia solani, pythium blight pathogen, fusarium graminearum, alternaria solani and carbon-color helminthosporium carposaprum, has the effects obviously better than the chemical disinfectant thiram. The raw materials adopted in the invention are purely natural plants, so that the preparation cost is low, and small-size industrial production can be carried out; furthermore, as the plants are Chinese herbal medicines, the polygonum aviculare botanical bactericide can be degraded rapidly after the use, is safe for people and live stock, good in environmental compatibility and free from secondary pollution to the environment, and meets the current concept of green food and environment sustainable development strategy.

The invention discloses banisterine benzoyl urea compounds and a preparation method and application thereof. The banisterine benzoyl urea compounds have a structural formula I shown in the specification; in the formula I, R1 can be methyl, phenyl, 3,4,5- triethoxy phenyl, p-methoxyphenyl or p-chlorphenyl, R2 can be H or Br, and X can be O or S. The banisterine benzoyl urea compounds have an outstanding insecticidal activity for Culex fatigans, prodenia litura and Chilo suppressalis; a part of the compounds are identical to a control insecticide Dibenzoyl-1-tert-butylhydrazine in term of activity; the banisterine benzoyl urea compounds have good inhibitory activity for rice sheath blight disease, alternaria solani, Collectotrichum musae, grey mould fruit rot of strawberry and sour rot pathogenic bacteria of citrus; a part of the compounds have better inhibitory activity for the above five phytopathogens than validamycin. The banisterine derivatives are simple in structure and easy to synthesize; the synthesis process is simple, the product purity is high; the banisterine benzoyl urea compounds and the preparation method thereof are suitable for large-scale industrial popularization and application.

The invention relates to a phenylimidazole derivative, a synthesis method of the phenylimidazole derivative and application of the phenylimidazole derivative to pesticide, particularly application tothe fungicide pesticide aspect, and belongs to the technical field of pesticide. In order to solve the technical problems, the invention provides a novel phenylimidazole derivative, a synthesis methodof the novel phenylimidazole derivative and application of the novel phenylimidazole derivative to the pesticide. The structure of the compound is shown as a formula I. The compound structure is simple and novel; the synthesis is easy; meanwhile, the fungicidal activity is realized; a better bacteriostasis or sterilization effect is achieved on important plant pathogenic fungi such as tomato early blight, tomato botrytis cinereal, cucumber fusarium oxysporum, magnaporthe grisea and rice rhizoctonia solani.

The invention relates to a 4-phenyl para-alcohol oxime ether-containing oxazoline compound as shown in the general formula (I), preparation of the compound and an application of the compound in pesticides. The compound represents a novel concise, wide-spectral and high-efficiency insecticidal anti-mite bactericidal herbicide structure type. When used as a novel insecticidal anti-mite bactericidal herbicide, the 4-phenyl para-alcohol oxime ether-containing oxazoline compound can greatly control oriental armyworm, cotton bollworm, corn borer, plutella xylostella, beet armyworm and mosquito larvae, can greatly control Tetrangychus cinnabarinus, can greatly inhibit ten pathogenic bacteria including cucumber fusarium wilt, Cercospora arachidicola, Physalospora piricola Nose, Alternaria solani, Fusarium graminearumt, late blight of potato, sclerotinia sclerotioru, Botrytis cinerea, rice sheath blight and phytophthora capsici, and can control and eliminate weeds including rape, amaranth, barnyard grass and crab grass, wherein the meaning of R is as shown in the specification.

The invention provides a Bacillus mojavensis QLY002 strain. The Bacillus mojavensis QLY002 strain has a preservation number of CGMCC No. 8905. The invention also provides a microbial inoculum of the Bacillus mojavensis QLY002 strain and its preparation method and use in potato pathogen inhibition and potato growth promotion. The Bacillus mojavensis QLY002 strain and the microbial inoculum can be used as microbe pesticides and fertilizer, can inhibit Phoma foveata, Alternaria solani, Fusarium oxysporum and Colletotrichum coccodes, have an antibacterial rate more than 61%, have potato growth promotion effects, can be used as potato special-purpose microbial pesticide and microbial fertilizer, solve the problem that the existing chemical pesticide has poor environmental friendliness, easily causes pesticide resistance and has poor safety, and have the advantages of good environmental friendliness, no pesticide resistance and good safety.

The invention discloses application of sirolimus in inhibition of plant alternaria solani and further discloses a fungicide. The fungicide contains rapamycin or a mixture of the rapamycin and a chemical fungicide. A TOR protein inhibitor rapamycin used in clinical medicine is used for prevention and control research of plant early blight for the first time, it is found through the research that the rapamycin and the mixture of the rapamycin and the chemical fungicide can effectively inhibit the plant alternaria solani, a chemical rapamycin pesticide can play a synergistic effect to inhibit the plant alternaria solani, a very significative base is provided for research of a novel drug for preventing and controlling the early blight, the novel fungicide is wide in application prospect and market prospect, a new choice is provided for early blight prevention and control, the using amount of the chemical pesticide can be remarkably decreased, and the fungicide has the great significance on reduction of pesticide usage amount and delay of the resistance of insect pests and also has the great application value on maintenance of ecosystem balance and sustainable development.

The invention relates to a method for separating and purifying the antibacterial peptide of an endophytic bacteria strain though high efficiency liquid chromatography, and the method comprises the following steps of: preparing the fermentation filter liquor of the strain by taking brevibacillus brevis B-011 as a basic strain, wherein the strain is collected in the China general microbiological culture collection center in April 12, 2013, and the collection number is CGMCC No.7466; adding acetonitrile; uniformly mixing, and standing; centrifugalizing; evaporating and concentrating a supernatant to obtain an antibacterial crude extract; and then carrying out secondary separation by adopting the high efficiency liquid chromatography to obtain an antibacterial peptide activity single-peak component. According to the invention, a bacteriostatic activity substance ( antibacterial peptide) contained in the fermentation filter liquor of the strain B-011 has inhibiting effect on the spore germination of alternaria solani and shows the teratogenic effect on germ tubes and hyphae. The method disclosed by the invention can be used for separating and purifying the bacteriostatic activity component contained in the strain fermentation filter liquor through the high efficiency liquid chromatography and identifying the substance and is conductive to defining the root cause of the strain on inhibiting the alternaria solani and developing the novel antibacterial drug.

The invention belongs to a method for preparing inula japonica plant extract and applications of the inula japonica extract. The plant material (root, stem, leaves, inflorescence and whole grass) of the inula japonica (including Inula japonica and Inula britannica) is crushed and extracted by a solvent which comprises 95% of alcohol; and the extract solution is concentrated to have the same weight with the inula japonica crushed matters, thus obtaining inula japonica extract. The inula japonica extract has obvious inhibition effects to cucumber botrytis cinerea, alternaria solani, cladosporium fulvum, colletotrichum lagenarium, sphaerotheca fuliginea, pseudoperonospora cubensis, potato phytophthora infestans, tomato phytophthora infestans and rhizoctonia cerialis; wherein the inflorescence extract has strong antibacterial activity. The inula japonica extract extracted by alcohol can be matched with suitable solvent and pesticide adjuvant to prepare britannica lactone emulsifiable solution and britannica lactione micro-emulsion of botanical fungicide. The inula japonica extract has obvious effects for preventing cucumber downy mildew, sphaerotheca fuliginea, tomato gray mold and tomato leaf mould, is especially suitable for prevention and control on the fungi diseases to economical crops such as vegetables and the like, and is a botanical fungicide with nuisanceless.

The invention discloses a plant growth-promoting rhizobacteria strain which is separated from dung of bos grunniens in Qinghai Province and preserved in the General Microorganisms Collection Center of China Committee for Culture Collection of Microorganisms with a preservation name of Bacillus subtilis QM34 and a preservation number of CGMCC No. 2669 on September 22, 2008; the strain QM34 has antagonistic action on 12 pathogenic bacteria of soil-born plant fungus; and the fermentation solution of the strain QM34 and the cell-free nutrient solution of the fermentation solution of the strain QM34 has obvious prevention and treatment effects on alternaria solani, significantly promotes the growth of tomato plants and can be colonized in a large quantity according to the tomato plant rhizosphere. Therefore, the rhizobacteria strain is a broad-spectrum growth-promoting rhizobacteria strain used or preventing and treating alternaria solani and most popular among people.

The invention discloses a multifunctional insecticide fertilizer for tomatoes and a preparation method of the multifunctional insecticide fertilizer. The multifunctional insecticide fertilizer is prepared from the following raw materials in parts by weight: 31-31.62 parts of furfural residue, 13-13.4 parts of urea, 21-21.6 parts of potassium sulfate, 23-23.5 parts of monoammonium phosphate, 1.8-2 parts of zinc sulfate, 1.8-2 parts of polyvinyl alcohol, 1.8-2 parts of polyacrylamide, 2.1-3.2 parts of bactericide, 0.2-0.4 part of citric acid, 0.06-0.08 part of compound sodium nitrophenolate and 0.16-0.2 part of EDTA. The multifunctional insecticide fertilizer for tomatoes disclosed by the invention is reasonably and especially designed according to the plentiful-lack indexes of nutrients of the soil in Hexi corridor in Gansu Province, and the absorption ratio of the tomatoes on nutrient elements; a series of problems that a greenhouse tomato field in Hexi corridor in Gansu Province is unbalanced in ratio of macroelements to microelements, low in organic matter content, and poor in water retaining capacity, and generates soil hardening, alternaria solani, late blight, gray mold, viral disease, bacterial wilt, septoria lycopersici and the like are solved, and the multifunctional insecticide fertilizer integrates nutrition, disease prevention, soil improvement and water retention.

The invention provides an alternaria solani PCR detection specific primer which comprises an upstream primer ASF: 5'-ACCACAAGGACCAACCCATA-3' and a downstream primer ASR: 5'-CCTACCTGATCCGAGGTCAA-3'. The invention further provides an alternaria solani PCR rapid detection method by using the primer, according to the method, a to-be-tested sample genome is taken as a template, the primer is used for PCR amplification, and a 409 bp amplified fragment can be detected by using gel electrophoresis after amplification is finished. The primer has high specificity, the sensitivity is high, the operation process is easy and convenient to operate, the detection requirement for alternaria solani on agricultural production can be met, and the defects that the operation is tedious, consumed time is long, the accuracy is low and the like in a conventional detection method are overcome.

The invention relates to a plant endophytic bacterium SH-1 and application thereof, belonging to the technical field of plant protection. The biocontrol strain Klebsiella variicola SH-1 was collected at China Center for Type Culture Collection on August 8, 2013; and the collection number is CCTCC NO: M2013367. The invention also relates to application of the plant endophytic bacterium SH-1 as a preparation for controlling tobacco brown spot, cabbage black spot and tomato early blight caused by alternaria strains. The plant endophytic bacterium SH-1 has the characteristics of being safe to humans, livestock and crops and being friendly to environment, has remarkable inhibition effect on growth of alternaria alternata (fries) keissler, alternaria oleracea M. Ibrath and alternaria solani, and has remarkable control effect on tobacco brown spot, cabbage black spot and tomato early blight caused by alternaria strains.

The invention belongs to the field of agricultural bactericides and herbicides, and particularly discloses triazole Mannich base compounds containing trifluoromethyl and piperazine, as well as preparation methods and application of the triazole Mannich base compounds. The triazole Mannich base compounds have structural formulas shown in formula I and formula II; in the structural formulas, R1 and R2 are defined as claim 1. The triazole Mannich base compounds have certain in-vitro inhibitory activities on such plant pathogens as cucumber fusarium wilt pathogens, peanut cercospora brown spot pathogens, apple ring spot pathogens, tomato early blight pathogens, wheat sharp eyespot pathogens and wheat scab pathogens, and particularly has relatively high in-vitro inhibitory activities on the apple ring spot pathogens and the wheat sharp eyespot pathogens. The triazole Mannich base compounds with the structural formulas shown in formula I and formula II both have certain weeding activities on oilseed rapes, and have relatively high in-vitro inhibitory activities on ketol-acid reductoisome-rase (KARI) of rice. The triazole Mannich base compounds are applicable to comprehensive control of the damage caused by bacteria and weeds, and inhibition on the KARI activity.

The invention discloses a 4-trifluoromethyl-7-hydroxycoumarin derivative as well as a preparation method and application thereof. The yield of the preparation method is high. The invention also discloses application of the 4-trifluoromethyl-7-hydroxycoumarin derivative to an agricultural anti-fungal aspect. An inventor detects the toxicity effect on rice sheath blight disease, colletotrichum lagenarium, fusarium graminearum, strawberry botrytis cinerea, apple spot germs and alternaria solani by a growth rate method; certain lateral reactivity is realized on the 4-trifluoromethyl-7-hydroxycoumarin derivative; the activity of individual compounds is superior to that of contrast medicine; the effect of the compound in the aspect of inhibiting the rice sheath blight disease is prominent; or the compound can become a lead compound molecule for developing and creating new pesticide.

The invention relates to a monoterpene phenolic derivative, a synthetic method of the monoterpene phenolic derivative and application of the monoterpene phenolic derivative in pesticides, in particular to application of the monoterpene phenolic derivative in the aspect of fungicide pesticides, and belongs to the technical field of pesticides. The technical aims of a new monoterpene phenolic derivative, a synthetic method of the new monoterpene phenolic derivative and application of the new monoterpene phenolic derivative in pesticides are achieved, and the structure of the compound is shown inthe formula I. The compound is simple in structure, novel, easy to synthesize, also has the fungicidal activity, and has a good bacteriostatic or sterilizing effect on important plant pathogenic fungi such as alternaria solani, botrytis cinerea, cucumber fusarium wilt, magaporthe grisea and rice sheath blight fungi.

The invention discloses 1,2,4-triazole Mannich base derivatives containing substituted piperazidine, and a preparation method and application thereof. The structural formulae of the 1,2,4-triazole Mannich base derivatives containing substituted piperazidine are disclosed as general formulae I and II, wherein X, Y and Z are defined as Claim 1. The compounds have high bactericidal activity, have high in-vitro inhibition activity for ring rot of apple, sheath blight of wheat, scab of wheat, early blight of tomato and other plant pathogens, have favorable in-vivo control effects on corynespora leaf spot of cucumber, bacterial spot of cucumber, gummy stem blight of cucumber, downy mildew of cucumber, sclerotinia rot of cucumber and other plant fungi, and especially have special effects for bacterial spot of cucumber and Pseudoperonospora cubensis Rostov. The compounds disclosed as general formula I and general formula II have weeding activity. The 1,2,4-triazole Mannich base derivatives are suitable for integrated control of fungus attack and crop smothering on various crops.

The invention discloses a method for detecting three potato pathogenic bacteria by multiple PCR (polymerase chain reaction) techniques, and belongs to the technical field of biological detection. According to the method, special genes of three pathogenic bacteria including phytophthora infestans, alternaria solani and rhizoctonia solani AG3 anastomosis groups are simultaneously amplified by multiple PCR once, and a PCR amplifying product is detected through agarose gel electrophoresis. The method includes the steps: (1) extraction of genome DNA (deoxyribonucleic acid) of the three potato pathogenic bacteria; (2) design of three pairs of amplification primers; (3) implementation of multiple PCR; (4) electrophoresis detection. According to the method, the three pathogenic bacteria are simultaneously detected by the multiple PCR techniques, and the method has the advantages that efficiency is high, speed is high, time is saved, and cost is reduced.

The invention belongs to the technical field of kind and molecule identification of plant pathogenic fungi, and aims to provide a detection kit for simply, rapidly and accurately identifying Alternaria solani Sorauer in potato blade tissues based on a polymerase chain reaction (PCR), and a detection method thereof. The detection kit comprises a pair of self-designed specific PCR primers and Mix, and the sequences of the specific primers comprise AsF:5'-CCCGCAAGGGGAGACAAA-3' and AsR:5'-CACCTCCCGGGGTGGCCA-3'. The detection method comprises the following steps: extracting blade or mycelium DNA, and carrying out PCR amplification by using the specific primer pair comprising AsF and AsF; carrying out agarose gel electrophoresis to analyze whether a 358bp strip specifically appears or not; and determining that whether a tested sample is Alternaria solani Sorauer according to the determined result of the above amplification strip. The method effectively solves the problems of Alternaria solani Sorauer field early-stage diagnosis and difficult kind identification caused by no spore production of isolates, realizes the early-stage diagnosis and detection of early blight, and provides technical support for scientific research people to molecularly identify the Alternaria solani isolates not producing spores.

The invention discloses applications of natural compound Murrayone as an agricultural bactericide. Natural compound Murrayone is taken as an agricultural bactericide used for preventing and treating agricultural fungal diseases, is capable of preventing and treating Sclerotinia sclerotiorum, Thanatephorus cucumeris, Botrytis cinerea, Fusarium graminearum, Colletotrichum gloesporioides, and Alternaria solani effectively, and possesses excellent treatment effects on fungal diseases such as Sclerotinia sclerotiorum, Thanatephorus cucumeris, and Colletotrichum gloesporioides. Agricultural bactericides prepared by taking natural compound Murrayone as an active ingredient possesses excellent curative effects, broad spectrum activity, and low toxicity, and are especially beneficial for environmental protection; no residue is left; and no pollution is caused.