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A plant growth-promotiong rhizosphere

A technology for promoting bacteria and plant roots, applied in plant growth regulators, plant growth regulators, bacteria, etc., can solve the problems of unstable control effect, lack of broad-spectrum bacteriostatic properties, and limited diversity of microbial resources. The effect of growth promotion and obvious control effect

Inactive Publication Date: 2009-04-08
山西师范大学
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, there are still some deficiencies in biological control as a whole: First, the sample materials used for screening are mainly concentrated in rhizosphere soil, infected diseased tissues, endophytes of susceptible plants, etc., which limits the diversity of microbial resources Second, the screened biocontrol bacteria often have a good antibacterial effect against a certain type or a certain type of pathogenic bacteria, and lack broad-spectrum antibacterial properties; third, changes in external conditions will directly affect the biocontrol agents. Biocontrol mechanism and biocontrol effect, that is, the control effect is unstable; fourth, the production and use technology of biocontrol bacteria is not perfect, etc.
Although some antagonistic bacteria with good control effects have been obtained abroad, there is no special screening of antagonistic microorganisms with tomato early blight as the control object. At present, it is limited to simple laboratory screening work in China. The antagonistic microorganisms are yeast mainly

Method used

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  • A plant growth-promotiong rhizosphere
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The isolation and screening test of embodiment 1QM34 bacterial strain

[0017] A plant rhizosphere growth-promoting bacterium, B. subtilis QM34, was isolated from Qinghai yak manure by gradient dilution method and screened by plate confrontation growth method. The specific implementation process includes: weighing 10.0g of Qinghai yak manure sample, grinding, adding to 90ml sterile water under aseptic conditions and shaking evenly, getting its supernatant and diluting it into a suitable (10 -5 、10 -6 、10 -7 ) concentration gradient, the gradient solution was heated in a water bath at 100°C for 10 minutes, spread and inoculated on beef extract peptone medium by conventional dilution method, and cultured at 37°C for 1 day, and the colonies with obvious differences in bacterial colony morphology were selected, and streaked and inoculated repeatedly On the corresponding agar plate, purify and obtain a single colony; use the PDA plate confrontation growth method for primar...

Embodiment 2

[0021] Pot control effect test of embodiment 2QM34 bacterial strain to tomato early blight

[0022] In the experiment, the fermented liquid and aseptic liquid of the QM34 strain were used to control tomato early blight respectively. Two treatments were set up for each control liquid. for front and back). Inoculated with sterile water as a control. All treatments were carried out simultaneously in three tomato varieties. The whole experiment was repeated twice and arranged randomly.

[0023] B. subtilis QM34 strain, inoculated in YPF-Ca liquid medium, 35°C, cultured in shake flask for 4 days, the concentration was about 10 9 cfu / ml, diluted 10 times is the fermentation broth of the QM34 strain; centrifuge the fermentation broth of the QM34 strain at 10,000rpm for 20 minutes to remove the precipitate, filter the supernatant with a microporous membrane (0.22um) to sterilize, and dilute it 3 times It is the sterile solution of QM34 strain. Tomato varieties are: No. 1, Dahong ...

Embodiment 3

[0027] The promotion test of embodiment 3 bacterial strain QM34 to tomato plant growth

[0028] Three kinds of tomato plants were inoculated with the QM34 fermentation broth with a dilution factor of 1, 10, and 20 times respectively, and the sterile water was used as a control. The number of leaves and plant height were measured from the day of inoculation, and the data were recorded every 7 days. After 28 days, each 2 plants of the average plant height of each treatment group were randomly selected, and the physiological indicators such as leaf size, root development, distance between leaves, and ground stem diameter were investigated (see Table 3).

[0029] The method of taking the root: soak the plant and the seedling pot in water together, then change the water and rinse it clean, get the root sample and take a picture; put the root in a transparent tray, and inject 3-5mm deep water, directly measure and Record the total root length; put the roots in an oven at 105°C for 1...

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Abstract

The invention discloses a plant growth-promoting rhizobacteria strain which is separated from dung of bos grunniens in Qinghai Province and preserved in the General Microorganisms Collection Center of China Committee for Culture Collection of Microorganisms with a preservation name of Bacillus subtilis QM34 and a preservation number of CGMCC No. 2669 on September 22, 2008; the strain QM34 has antagonistic action on 12 pathogenic bacteria of soil-born plant fungus; and the fermentation solution of the strain QM34 and the cell-free nutrient solution of the fermentation solution of the strain QM34 has obvious prevention and treatment effects on alternaria solani, significantly promotes the growth of tomato plants and can be colonized in a large quantity according to the tomato plant rhizosphere. Therefore, the rhizobacteria strain is a broad-spectrum growth-promoting rhizobacteria strain used or preventing and treating alternaria solani and most popular among people.

Description

technical field [0001] The invention relates to a plant rhizosphere growth-promoting bacterium, which can broadly prevent and treat various plant soil-borne fungal diseases, can colonize a large number of plant rhizospheres, and has obvious promoting effect on plant growth and development, and belongs to the technical field of agricultural microorganisms. It also belongs to the technical field of plant biological control. Background technique [0002] Since the late 1960s, with the long-term irrational use of a large number of chemical pesticides, the chemical control of plant diseases and insect pests has seriously threatened the development of agricultural economy and human health, and has become a serious challenge for human society entering the 21st century. [0003] With the development of science and technology, facing the deteriorating environment, people's understanding of harmful organisms and crop protection strategies have changed, from "extermination" to "moderat...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/125
Inventor 胡青平徐建国
Owner 山西师范大学
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