Alternaria solani Sorauer detection kit and detection method thereof

Abstract

The invention belongs to the technical field of kind and molecule identification of plant pathogenic fungi, and aims to provide a detection kit for simply, rapidly and accurately identifying Alternaria solani Sorauer in potato blade tissues based on a polymerase chain reaction (PCR), and a detection method thereof. The detection kit comprises a pair of self-designed specific PCR primers and Mix, and the sequences of the specific primers comprise AsF:5'-CCCGCAAGGGGAGACAAA-3' and AsR:5'-CACCTCCCGGGGTGGCCA-3'. The detection method comprises the following steps: extracting blade or mycelium DNA, and carrying out PCR amplification by using the specific primer pair comprising AsF and AsF; carrying out agarose gel electrophoresis to analyze whether a 358bp strip specifically appears or not; and determining that whether a tested sample is Alternaria solani Sorauer according to the determined result of the above amplification strip. The method effectively solves the problems of Alternaria solani Sorauer field early-stage diagnosis and difficult kind identification caused by no spore production of isolates, realizes the early-stage diagnosis and detection of early blight, and provides technical support for scientific research people to molecularly identify the Alternaria solani isolates not producing spores.

Description

technical field [0001] The invention relates to the technical field of species molecular identification of plant pathogenic fungi. Background technique [0002] Potato early blight caused by Alternaria solani[ Alternaria solani Sorauer (Ellis)] is caused by this disease, which can harm potato leaves, above-ground stems and underground tubers, and is a worldwide disease. The loss caused by potatoes in general years is between 5-78%, and severe areas can also cause crop failure. Early diagnosis of potato early blight is of great significance for the prevention and control of the disease, and the symptoms of leaves and tissues invaded by early blight bacteria develop slowly and are not obvious, and are easy to be confused with other diseases. Most of the obtained early blight pathogens do not produce sporulation, and it is difficult to identify their species. The in vitro induction of early blight pathogens has a long period of sporulation and a large workload, so it is diffic...

AI Technical Summary

Problems solved by technology

Early diagnosis of potato early blight is of great significance for the prevention and control of the disease, and the symptoms of leaves and tissues invaded by early blight bacteria develop slowly and are not obvious, and are easy to be confused with other diseases. Most of the obtained early blight pathogens do not produce sporulation, and it is difficult to identify their species. The in vitro induction of early blight pathogens has a long period of sporulation and a large workload, so it is difficult to adapt to the field detection of early blight and the types of non-sporulation isolates. identification needs