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Preparation method and application of inula japonica extract and combination thereof

A technology of inula flower and extract is applied in the field of preparation and use of inula flower extract and composition thereof, and achieves the effects of low cost, easy availability of raw materials and easy cultivation.

Inactive Publication Date: 2009-09-02
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the preparation of botanical fungicides with inula lactone as the active ingredient to prevent and control plant fungal diseases by using inula extracts has not been found. , Cucumber anthracnose, Cucumber powdery mildew, Cucumber downy mildew, Tomato early blight, Tomato leaf mold, Tomato infestation, Potato infestation and Wheat sheath blight etc. inulalide

Method used

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  • Preparation method and application of inula japonica extract and combination thereof
  • Preparation method and application of inula japonica extract and combination thereof
  • Preparation method and application of inula japonica extract and combination thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076]In August 2005, the roots, stems, leaves, flowers, and whole plants (aboveground parts) of Inula inula in full bloom stage were collected from the suburbs of Chengde City, Hebei Province, dried in the shade at room temperature, and crushed to 40 mesh dry powder. Weigh 1 part each of Inula inula (I.japonica) and Inula inula (I.britannica) root, stem, leaf, inflorescence, and whole grass dry powder, each 50g, according to the ratio of dry powder to solvent 1:10 (g / ml ) were added with 95% ethanol, and extracted by shaking (50° C., 150 r / m) for 3 hours. Suction filtration, and the filtrate was concentrated under reduced pressure (60°C, 0.1 atmosphere) to obtain extract. The experiment was carried out 3 times. The results showed that there was no significant difference in the extract yields from the same extraction parts of the two Inula flowers (Table 1), the extract yield was the highest in the inflorescence, and the extract extract yield in the roots and stems was the lo...

Embodiment 2

[0080] Weigh 6 parts of Inula japonica inflorescences, 1 kg each, add 10 liters of 95% ethanol, shake and extract (50° C., 150 r / m) for 1, 2, 3, 4, 5, 24 hours. Suction filtration, and the filtrate was concentrated under reduced pressure (60°C, 0.1 atmosphere) to obtain extracts 52.2, 66.1, 75.4, 77.2, 77.9, and 80.9g extracted with 95% ethanol, and the yields were 5.22%, 6.61%, 7.54%, and 7.72% respectively , 7.79%, 8.09%. Weigh 6 parts of Inula britannica inflorescences, 1 kg per part, and use the same method to extract to obtain 95% ethanol extraction extract yields are 5.30%, 6.60%, 7.43%, 7.69%, 7.81%, 8.13% .

[0081] Table 2, Inula inflorescence extraction paste yield at different times

[0082]

[0083] Note: The experiment was repeated 3 times, and the yield is the average of 3 repetitions. Different letters after the values ​​in each column indicate significant differences between the values ​​(p=0.05). The same below.

[0084] The results showed that there w...

Embodiment 3

[0086] Prepare 4 parts of inflorescence dry powder of Inula japonica and Inula britannica (Inula britannica), each 50g, add 95% ethanol and shake and extract 1, 2, 3, 4 times (50°C, 150r / m) , 3 hours each time. The ratio of dry powder mass (g) to solvent volume (ml) for the first time is 1:10, and the ratio of dry powder mass (g) to solvent volume (ml) for the second, third, and fourth times is 1:8. Filtrate with suction, combine the filtrates, and concentrate the filtrate under reduced pressure (60°C, 0.1 atmosphere) to obtain an extract. The experiment was repeated three times. The results showed that there was no significant difference in the yields of the extracts obtained by the same number of extractions of the two Inula flowers with 95% ethanol, and the amount of extracts obtained by extracting 2-3 times was higher (Table 3).

[0087] Table 3, Inula inflorescence extracts the paste yield of different times with 95% ethanol

[0088]

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Abstract

The invention belongs to a method for preparing inula japonica plant extract and applications of the inula japonica extract. The plant material (root, stem, leaves, inflorescence and whole grass) of the inula japonica (including Inula japonica and Inula britannica) is crushed and extracted by a solvent which comprises 95% of alcohol; and the extract solution is concentrated to have the same weight with the inula japonica crushed matters, thus obtaining inula japonica extract. The inula japonica extract has obvious inhibition effects to cucumber botrytis cinerea, alternaria solani, cladosporium fulvum, colletotrichum lagenarium, sphaerotheca fuliginea, pseudoperonospora cubensis, potato phytophthora infestans, tomato phytophthora infestans and rhizoctonia cerialis; wherein the inflorescence extract has strong antibacterial activity. The inula japonica extract extracted by alcohol can be matched with suitable solvent and pesticide adjuvant to prepare britannica lactone emulsifiable solution and britannica lactione micro-emulsion of botanical fungicide. The inula japonica extract has obvious effects for preventing cucumber downy mildew, sphaerotheca fuliginea, tomato gray mold and tomato leaf mould, is especially suitable for prevention and control on the fungi diseases to economical crops such as vegetables and the like, and is a botanical fungicide with nuisanceless.

Description

Technical field: [0001] The invention belongs to the preparation method and application of plant material extract and composition thereof. In particular, it relates to ethanol extracts obtained by solvent extraction of plant material of Inula japonica (including Inula britannica), processes for their preparation, compositions containing such ethanol extracts and the use of ethanol extracts and compositions for the control of Use for fungal diseases of plants. Background technique: [0002] Inula (including Inula japonica and Inula britannica) belongs to the Asteraceae family. It has the effects of eliminating phlegm, lowering qi, softening firmness and promoting water flow. It has been reported in the literature that the aerial parts of Inula inula contain various components such as inulalide, germanolactone, quercetin, quercetin, caffeic acid, and chlorogenic acid (Huang Taikang, Handbook of Commonly Used Traditional Chinese Medicine Components and Pharmacology, p1608 , ...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01N25/04A01P3/00
Inventor 王文桥韩秀英张小风马志强李红霞王红刚张文渊
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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