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Application of aucubin to preparation of medicines for treating renal interstitial fibrosis

A technology for renal interstitial fibrosis and aucubin, applied in the field of new application of aucubin, can solve the problems of many steps, large consumption of reagents, cumbersome operation, etc.

Active Publication Date: 2014-10-29
CHANGSHA DUXACT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many mature preparation methods for aucubin in the prior art, but at present, there are few researches on the process of extracting aucubin from Eucommia samara
Chinese patent 201010297478.7 discloses "a process for extracting aucubin from Eucommia ulmoides", which mainly includes flash or ultrasonic extraction, macroporous resin adsorption and purification, but the purity of aucubin obtained by this process is only 50% , and the consumption of reagents is large, the operation is cumbersome, the industrial production cycle is long, and the cost is high, which is not conducive to the industrial production, development and utilization of aucubin monomer
Chinese patent 201310028642.8 discloses "a method for the rapid and efficient preparation of aucubin monomer from Eucommia ulmoides." This method can produce aucubin through two steps of extraction and crystallization, but the yield is low and the waste of resources is serious
Chinese patent CN 101863938 A discloses "a method for preparing high-purity aucubin". The method uses a polymer-filled stationary phase chromatographic column, silica gel column chromatography, and recrystallization to separate and obtain aucubin. There are many steps, The product yield is low and the production cycle is long

Method used

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  • Application of aucubin to preparation of medicines for treating renal interstitial fibrosis
  • Application of aucubin to preparation of medicines for treating renal interstitial fibrosis
  • Application of aucubin to preparation of medicines for treating renal interstitial fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Eucommia ulmoides Samara Extract Aucubin

[0031] Shell the Eucommia samara, crush the nuts to 40 mesh, take 10kg and put it into the extraction tank, add 60L petroleum ether to degrease, degrease twice, each time for 3 hours, filter out the dregs as degreasing coarse material. Add 80L of ethanol to ultrasonically extract the defatted crude material for 2 hours, extract 3 times, and combine the extracts. The extract was concentrated under reduced pressure at 40°C to 1 / 8 of the original volume and ethanol was recovered. The concentrated solution was saturated with acetone and dissolved, crystallized at 7°C for 48 hours, and the crude crystals were saturated with ethyl acetate and dissolved. Get 10L silica gel packing column, add saturated liquid to silica gel column, use 3BV ethyl acetate (mass ratio ethyl acetate:methanol=1:0) successively, 4BV10:1 ethyl acetate / methanol (mass ratio ethyl acetate:methanol= 10:1), 5BV6:1 ethyl acetate / methanol ...

Embodiment 2

[0032] Example 2 Preparation of Eucommia ulmoides Samara Extract Aucubin

[0033] Dehull Eucommia samara, crush the nuts to 50 mesh, take 10kg and put it into the extraction tank, add 80L petroleum ether to degrease, degrease 3 times, each time for 4 hours, filter out the dregs as degreasing coarse material. Add 100L of ethanol to ultrasonically extract the defatted crude material for 4 hours, extract 5 times, and combine the extracts. The extract was concentrated under reduced pressure at 40°C to 1 / 10 of the original volume and ethanol was recovered. The concentrated solution was saturated with acetone and dissolved, crystallized at 4°C for 72 hours, and the crude crystals were saturated with ethyl acetate and dissolved. Get 10L silica gel packing column, add saturated liquid to silica gel column, use 3BV ethyl acetate (mass ratio ethyl acetate:methanol=1:0) successively, 4BV10:1 ethyl acetate / methanol (mass ratio ethyl acetate:methanol= 10:1), 5BV6:1 ethyl acetate / methanol...

Embodiment 3

[0035] The aucubin obtained in Example 1 and Example 2 and commercially available aucubin (purchased from Shanghai Yuanye Biotechnology Co., Ltd.) were used in the experimental research on the protection of renal interstitial fibrosis in Example 3 .

[0036] 1. Experimental method

[0037] 1.1 Dose Design and Grouping

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PUM

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Abstract

The invention relates to the field of applications of traditional Chinese medicine extracts and in particular provides an application of aucubin to preparation of medicines for treating renal interstitial fibrosis. Aucubin is preferably the eucommia ulmoides samara extract, wherein the purity of aucubin is above 98%.

Description

technical field [0001] The invention belongs to the field of use of extracts of traditional Chinese medicines, and in particular relates to a new use of aucubin. Background technique [0002] Renal fibrosis is a pathophysiological change, which is a gradual process in which the function of the kidney changes from healthy to damaged, then damaged, and finally lost. The result is progressive and irreversible damage to kidney function, which poses a huge threat to human health. Renal interstitial fibrosis is a common pathological feature of renal disease progressing to end-stage renal failure due to various reasons. A large number of clinical and pathological data show that chronic renal injury caused by various reasons is more closely related to the severity of renal interstitial fibrosis than glomerular lesions. Therefore, in order to slow down and reverse the progress of chronic kidney disease and improve renal function, it is of great significance to discover and find eff...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P13/12C07H17/04C07H1/08
Inventor 严谨郭成贤姜德建胡凯李晓晖田莹莹冯晗王亚芹
Owner CHANGSHA DUXACT BIOTECH CO LTD
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