Method for extracting active ingredients for reducing sugar blood from waste residues of liquorice
An active ingredient and a hypoglycemic technology, which is applied in the field of extracting hypoglycemic active ingredients, can solve problems such as environmental pollution and resource waste, and achieve the effects of low cost, low cost and good medicinal prospect.
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[0017] Experiment 1 Screening of extraction technology of licorice flavonoids in licorice waste residue
[0018] (1) Two technical routes of organic solvent thermal reflux method:
[0019] Take 100 g of dried licorice slag, add 2000 ml of 95% ethanol solution with a concentration of 95%, water-bath reflux temperature of 65 ° C, time of 60 min, filter, extract a total of 3 times, combine the filtrates, and concentrate under reduced pressure to obtain a concentrated solution;
[0020] a. Extract the concentrated solution 3 times with an equal volume of petroleum ether, discard the petroleum ether layer, add an equal volume of ethyl acetate and extract 3 times to obtain an ethyl acetate extract A 1 . will A 1 Wash with 5% sodium carbonate solution (adjusted to pH 8-9), then add 5% hydrochloric acid solution (adjusted to pH 5-6), let stand for precipitation, and the solution is A 2 , which precipitates A 3 ;
[0021] b. The concentrated liquid extract is subjected to 30-60 me...
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