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Method for extracting chlorogenic acid and pinoresinol from eucommia barks

A technology of bark of Eucommia ulmoides and chlorogenic acid, which is applied in the field of extracting chlorogenic acid and pinoresinol diglucoside, and extracting chlorogenic acid and pinoresin, can solve the problems of large impact and target object impact, reduce production cost, improve Effect of adding value and improving utilization efficiency

Inactive Publication Date: 2014-05-21
LUXI XIAOYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, enzymatic hydrolysis has a great influence on easily degradable substances such as pinoresin, and at the same time, the use of decompression recovery to remove the solvent will also have a certain impact on the target substance.
[0004] Geniposidic acid, chlorogenic acid and pinoresinol diglucoside are easy to be oxidized and decomposed under high temperature conditions. Due to the high content of chlorogenic acid in Eucommia bark, some chlorogenic acid can also be obtained by conventional methods, but pinoresinol diglucoside Substances with low content such as glucoside and geniposidic acid are difficult to obtain by conventional methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Use 100kg of processed ulmoides bark to crush to below 10 mesh, add 250L of 55% methanol aqueous solution (the concentration of ascorbic acid is 50mmol / L) and extract at 50°C for 60min, filter once with a ceramic membrane, and add the same extract to the filter residue again Repeat the extraction once, combine the two filtrates to obtain 420L of filtrate I; add 200L of 20% ethanol aqueous solution (in which the concentration of hydrochloric acid is 0.01mol / L) to the filter residue for mixed extraction, extract at 50°C for 60min, and filter once with a ceramic membrane to obtain 160L of filtrate II; Ⅰ and Ⅱ were respectively carried out at 50°C in a rising film concentrator (Hangzhou Huihe Machinery Equipment Co., Ltd.) from 55°C to 50L and 20L; after the filtrate Ⅰ was concentrated, it was adsorbed on DM301 macroporous resin and eluted with 30% ethanol solution to obtain Eluent I was 200L, eluted with 70% ethanol solution to obtain eluent II 150L; filtrate II was concent...

Embodiment 2

[0027]Use 200kg of processed ulmoides bark to crush to below 10 mesh, add 500L of 55% methanol aqueous solution (the concentration of ascorbic acid is 40mmol / L) and extract at 50°C for 60min, filter once with a ceramic membrane, and add the same extract to the filter residue again Repeat the extraction once, combine the two filtrates to obtain 860L of filtrate I; add 400L of 20% ethanol aqueous solution (in which the concentration of hydrochloric acid is 0.01mol / L) to the filter residue for mixed extraction, extract at 50°C for 60min, and filter once with a ceramic membrane to obtain 350L of filtrate II; Ⅰ and Ⅱ were respectively carried out at 50°C in a rising film concentrator (Hangzhou Huihe Machinery Equipment Co., Ltd.) from 55°C to 100L and 45L; after the filtrate Ⅰ was concentrated, it was adsorbed on DM301 macroporous resin and eluted with 30% ethanol solution to obtain Eluent I was 350L, and 70% ethanol solution was eluted to obtain eluent II 250L; filtrate II was conc...

Embodiment 3

[0029] Use 400kg of processed ulmoides bark to crush to below 10 mesh, add 1000L of 55% methanol aqueous solution (the concentration of ascorbic acid is 60mmol / L) and extract at 50°C for 60min, filter once with a ceramic membrane, and add the same extract to the filter residue again Repeat the extraction once, combine the two filtrates to obtain 1500L of filtrate I; add 800L of 20% ethanol aqueous solution (in which the concentration of hydrochloric acid is 0.005mol / L) to the filter residue for mixed extraction at 50°C for 60min, filter once with a ceramic membrane, and obtain 720L of filtrate II; Ⅰ and Ⅱ were respectively carried out at 50°C in a rising film concentrator (Hangzhou Huihe Machinery Equipment Co., Ltd.) from 55°C to 180L and 80L; after the filtrate Ⅰ was concentrated, it was adsorbed on DM301 macroporous resin and eluted with 30% ethanol solution to obtain Eluent I was 700L, 70% ethanol solution was eluted to obtain eluent II 500L; filtrate II was concentrated an...

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PUM

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Abstract

Aiming at the phenomena that pinoresinol in the extraction process is easy to decompose to severely affect production of the pinoresinol on a large scale, the method provided by the invention separates and purifies pinoresinol and chlorogenic acid gradually by adopting ethanol extraction, rising film concentration, column chromatography and chromatographic separation, so that the requirements on production of the pinoresinol and chlorogenic acid on a large scale are realized, thereby effectively improving the yield of pinoresinol in eucommia leaves and meanwhile purifying high content chlorogenic acid.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting chlorogenic acid and pinoresin, and more specifically relates to a method for extracting chlorogenic acid and pinoresinol diglucoside from bark of Eucommia ulmoides. Background technique [0002] Eucommia ulmoides is a woody deciduous plant with a variety of medicinal functions. It has extremely high medicinal value and has been used as medicine for more than 2,000 years. Sixian". The chemical components of Eucommia are mainly lignans, cycloalkenyl ether mushroom glycosides, phenols, volatile oils, polysaccharides, amino acids, organic acids, pylitol, phospholipids, flavonoids, vitamins, trace elements, and Eucommia gum. Among them, the lignans include pinoresinol diglucoside, syringareol diglucoside, olive oil and eucommia A, B and so on. Pinoresinol diglucoside and syringareol diglucoside have two-way regulation function on animal blood pressure...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07D307/12
CPCC07C67/48C07C2601/14C07D307/12C07D493/04C07C69/732
Inventor 向华
Owner LUXI XIAOYUAN BIOTECH
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