37 results about "Rhynchophylline" patented technology

The present invention provides a preparation method for high purity rhynchophylline. The method comprises: crushing rhynchophylline medicinal material to have a particle size of 20-60 meshes; adding the crushed rhynchophylline to a sodium carbonate solution, wherein the amount of the sodium carbonate solution is 4-5 times the amount of the crushed rhynchophylline, and the sodium carbonate content in the sodium carbonate solution is 5%; carrying out ultrasonic extraction for 1 hour, and removing liquid through filtering; adding dichloromethane to carry out ultrasonic extraction for 2-3 times, recovering the regent from the extracting solution; concentrating the extracting solution to small volume; carrying out separation through high speed countercurrent chromatography, carrying out online monitoring through an ultraviolet detector; collecting the target component; carrying out cold preservation and crystallization to obtain the high purity rhynchophylline. With the method provided by the present invention, the separation treatment is performed through the high speed countercurrent chromatography; the production period is short; the product yield is high; the product purity is more than 95%.

The invention belongs to the technical field of medicine detection and particularly relates to a method for quickly detecting ramulus uncariae cum uncis by utilizing near-infrared spectrometry and application of the method. The method comprises the following steps: (1) crushing the selected ramulus uncariae cum uncis with known content of moisture, extract, rhynchophylline and isorhynchophylline, and sieving for later use; (2) collecting near infrared spectrum data of the ramulus uncariae cum uncis powder; (3) carrying out correlation on the selected spectral information under a characteristic wave band and the known content of moisture, extract, rhynchophylline and isorhynchophylline of ramulus uncariae cum uncis, and establishing a quantitative calibration model by adopting a partial least square method; and (4) performing near infrared spectrum scanning on an unknown ramulus uncariae cum uncis sample according to the method in the step (2), and introducing the selected spectral information under the characteristic wave band into the established quantitative calibration model to obtain the content values of the moisture, the extract, the rhynchophylline and the isorhynchophylline of the unknown ramulus uncariae cum uncis sample. According to the method for quickly detecting ramulus uncariae cum uncis by utilizing near-infrared spectrometry and the application of the method disclosed by the invention, quick and complete detection on ramulus uncariae cum uncis is realized by introducing a near-infrared spectrometry technology. Moreover, the method has the advantages of simplicity in operation, high accuracy and high precision.

The invention relates to a process for extracting rhynchophylline monomers from uncaria rhynchophylla. The process comprises the following steps of: pretreating uncaria rhynchophylla medicinal materials by adopting a lower alcohol water quenching ultrasonic method to prepare a crude extract; separating rhynchophylline in the crude extract by adopting a silicagel column chromatography method by using two organic reagents and ammonia water according to a certain proportion as an eluent; and finally obtaining rhynchophylline monomers through steps of eluting fractions, extracting, purifying, and the like. The method has the advantages of safety, high efficiency, low cost and high rhynchophylline monomer productivity; and per kilogram of the medicinal materials can be used for obtaining 0.27g rhynchophylline monomers, and the purity is higher than 90 percent, so that the method is beneficial to industrial large-scale production and provides an approach for the further development of rhynchophylline as a new drug.

The invention belongs to the field of natural medicine chemistry, and particularly relates to a preparation method of isorhynchophylline. The method comprises the following steps: 1) crushing an uncaria medicinal material, acidifying the material by 1% hydrochloric acid, performing reflux extraction with 70-80% ethanol for 2-3 times, concentrating and ammonifying the extract to obtain a pH of 9-10, standing and precipitating to obtain a precipitate; 2) separating the precipitate by high speed countercurrent chromatography, performing on-line monitoring by an ultraviolet detector, collecting the object components, performing refrigeration and crystallization to obtain high-purity rhynchophylline. The preparation method of isorhynchophylline of the invention has a simple process, easy operations, a short preparation period, and high product purity, and is applicable to continuous production.

The invention discloses a planting method of uncaria rhynchophylla and relates to the technical field of Chinese herbal medicine planting. The planting method includes the steps of: collecting seeds, threshing and storing the seeds, processing the seeds, sowing the seeds, transplanting small seedlings, performing nursery management, performing final singling of seedlings and middle-stage management, and harvesting the uncaria rhynchophylla. In the method, the uncaria rhynchophylla sprouts in 3-4 days after sowing the seeds, so that the method greatly reduces the growth period than conventional sowing methods, in which budding period lasts for 3 months from sowing the seed in the last ten-days of March to sprouting in the middle ten-days of June. Meanwhile, the method can enrich magnetotactic microorganisms, which are key factors for stimulating sprouting of seeds and reinforcing seedlings, and establish an abundant microelement substance basis, so that the plants can be normally harvested with no need for a period of 3 years. Contents of active components, such as rhynchophylline and isorhynchophylline, in the uncaria rhynchophylla is not less than those in plants in a conventional planting method, even wild plants; according to the planting method, the seeds are sowed in spring and the plants are harvested in autumn in the third year, planting period lasting for about two and a half years; the method solves a problem of long planting period that the uncaria rhynchophylla can only be normally harvested after being planted for 3 years.

The invention discloses a granule used for syndromes after brain injuries and a preparation method thereof.The granule used for syndromes after brain injuries is prepared from, by weight, diosgenin, isorhamnetin, coumarona odoruta, kaempferol, coral nano powder, rhynchophylline, astragaloside, cerebroprotein hydrolysate, tolperisone hydrochloride, limonene, taxifolin, gamma-aminobutyric acid, glutamic acid, oryzanol, a filling agent, an adhesive, a disintegrating agent and a lubricating agent.Various medicine effective components are combined, by means of a reasonable ratio, the granule has the functions of easing pain and calming the nerves, relieving restlessness and strengthening the brain, calming the heart and nourishing qi, activating blood circulation to remove blood stasis, tonifying brain nerves and preventing fatigue, and symptoms caused when the syndromes after brain injuries occur can be relieved.Besides, brain nerve nutrient can be enhanced, symptomatic treatment and conditioning are combined, pain of a patient is relieved, mood can be relieved, and the treatment effect of healing is achieved.

The invention provides application of an uncaria active compound in preparation of a medicine for preventing and/or treating mental disorder diseases, and relates to the technical field of medical application. The mental disorder diseases comprise neurological and mental disorders caused by traumatic memory. The uncaria active compound disclosed by the invention can comprise rhynchophylline, isorhynchophylline, and derivatives, isomers and the like obtained by modifying molecular structures of the active compounds. The active compound provided by the invention can promote fading of wound memory, and has prevention and alleviation effects on neuropsychiatric disorders (such as post-traumatic stress disorder and phobia) and the like caused by the wound memory.

The invention relates to an antimicrobial-free selenium-enrichment piglet mixed feed additive preparation method. A formula of the antimicrobial-free selenium-enrichment piglet mixed feed additive comprises the ingredients by weight percent: 1.8 to 2.8% of L-tyrosine, 2.3 to 3.3% of natural vitamin E, 1.2 to 2.2% of rhizoma pinelliae, 2.3 to 3.3% of lactobacillus buchneri, 1.5 to 2.5% of trisodiummonohydrogen, 2.5 to 3.5% of saw palmetto extractive, 2 to 2.8% of bacillus laterosporus, 1.3 to 2.3% of mile swertia herb, 2.6 to 3.6% of radix berberidis, 1 to 2% of enterobacter cloacae, 0.5 to 1.5% of calcium propionate, 1.2 to 2.2% of radix angelicae sinensis, 0.3 to 1.3% of dimethyl ancymidol menadione sulfite, 0.8 to 1.8% of bacillus lentus, 0.9 to 1.7% of manganese carbonate, 0.6 to 1.6%of snake gall extractive, 0.9 to 1.8% of chromium yeast, 1.2 to 2.2% of rhynchophylline, 0.9 to 1.9% of probiotic, 0.6 to 1.6% of selenium yeast and 73.6 to 54.1% of water. The antimicrobial-free selenium-enrichment piglet mixed feed additive disclosed by the invention has better functions of promoting mitosis and differentiation proliferation of lymphocyte and macrophage, increasing cell factorsand maintaining intestinal mucosa completeness and mammary epithelial cells.

The invention relates to novel application of a medicine, in particular to application of Rhy (rhynchophylline) to preparation of medicines for improving vascular endothelial dysfunction. Incubation of renal arteries of SHRs (spontaneously hypertensive rats) is realized by application of the rhynchophylline, an improvement function of the rhynchophylline to endothelium-dependent vasodilation of the renal arteries is assessed by a multi-channel small vessel tension tester, and by which approach the rhynchophylline gives play to an improvement effect on renal vascular endothelial vasodilation dysfunction associated with hypertension is detected by a western blotting method and an NO detection kit. According to researches, the endothelium-dependent vasodilation of the renal arteries of the SHRs can be improved by incubation with the rhynchophylline in time and concentration dependency, and the improvement function of the rhynchophylline is realized by activating a Src-PI3K/Akt-eNOS passage to improve eNOS activity.

A rhynchophylline preparation process includes: (1) soaking ground Ramulus Uncariae cum Uncis in ethyl alcohol and hydrochloric acid mixed solution; (2) heating and concentrating extract liquid to a non-alcohol state, cooling, adjusting PH to 8-10, adding chloroform, and extracting to obtain a low-content rhynchophylline crude product; (3) preliminarily separating through normal-phase silica gel chromatography to obtain a high-content rhynchophylline crude product; (4) dissolving the rhynchophylline crude product in hydrochloric acid solution, and adopting a reverse-phase C18 medium-pressure column for purification to obtain high-purity rhynchophylline hydrochloride; (5) reducing the rhynchophylline hydrochloride into rhynchophylline, filtering after separating out, washing off excessive ammonium hydroxide and salt with pure water, and directly performing freeze drying to obtain a high-purity rhynchophylline product. The rhynchophylline preparation process has advantages that (1) organic reagent extraction is mostly avoided, cost is reduced, and harms of organic reagents are reduced; (2) stability of rhynchophylline in a preparation process is guaranteed, and the target yield is increased; (3) the high-purity product is obtained.

The invention discloses an ointment for treating acne, which is prepared from the following raw materials in parts by weight: 20 to 40 parts of frond of endarachne binghamiae, 30 to 50 parts of hedgeglorybind root, 15 to 22 parts of pepperweed seed, 5 to 15 parts of fruit of cerasus humilis, 10 to 16 parts of rhynchophylline, 11 to 16 parts of winterberry euonymus herb, 3 to 8 parts of phoenix tree leaf, 2 to 6 parts of wolfberry fruit extract and 10 to 15 parts of fructo-oligosaccharide. By compounding the raw materials to exert a synergistic effect, the ointment disclosed by the invention can effectively treat adolescent acne, repair scars and promote skin regeneration, the treatment period is short, and the curative effect is good; an external application method is adopted, toxic and side effects are minor, the treatment cost is reduced, and the ointment can help protect the physical and metal health of teenagers.

The invention relates to a method for extracting a vasodilatation active compound rhynchophylline. The method comprises the following steps: (1), grinding the raw materials and sieving to obtain a raw material powder, adding the raw material powder into an organic solvent, importing carbon dioxide to form a swelling solvent system, and stirring and extracting to obtain an extracting solution; (2), concentrating the extracting solution to obtain a primarily extracted concentrate; (3), adding an inorganic matter solution and an organic solvent in the primarily extracted concentrate to carry out liquid-liquid extraction, layering to obtain an organic phase I, adding water into the organic phase I, stirring uniformly, layering to obtain an organic phase II and concentrating the organic phase II to obtain an extract; (4), dissolving the extract and filling to the top of a chromatography column, carrying out solvent elution, collecting the eluant, concentrating to obtain a crude product, and recrystallizing to obtain the rhynchophylline. According to the method, the obtained rhynchophylline is favorable in yield and purity, has a favorable application prospect and is potential in industrial production.

The present invention discloses a hypotensive pharmaceutical composition. The composition is prepared by dissolving daidzein, honokiol, soybean phosphatidylcholine, cholesterol, DSPE-PEG in an organicsolvent, then preparing a liposome suspension by a membrane-sonic method, then adding hydrochlorothiazide, rhynchophylline and a diluent into the liposome suspension, performing freeze-dying, uniformly mixing the dried material with a disintegrant and a lubricant, and finally performing tabletting. The invention uses the daidzein and the honokiol to prepare the encapsulated liposome so as to delay the release rate of the drug, and the liposome can be released simultaneously with the hydrochlorothiazide and the rhynchophylline, thereby improving the coordination of the action of drug components in the body and increasing the curative effect.

The invention discloses application of rhynchophylline in preparation of a medicine for treating inflammatory enteritis. The invention firstly finds that the rhynchophylline has a repairing effect on injury caused by inflammatory enteritis; research finds that rhynchophylline can significantly reduce the concentration of inflammatory factors in colon, protect colon tissue, repair intestinal barrier permeability and activate AhR, so that intestinal inflammation can be relieved, and the intestinal barrier permeability can be repaired. The invention provides an application prospect of rhynchophylline in preparation of medicines for treating inflammatory enteritis.