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Method of determining content of total alkaloid in uncaria macrophylla extract

A determination method and technology of total alkaloids, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as accuracy disputes, and achieve the effects of high accuracy, good reproducibility, and high precision

Inactive Publication Date: 2019-09-03
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly titration methods, direct ultraviolet spectrophotometry and acid dye colorimetric methods for the determination of the total alkaloid content of Uncaria chinensis, but the accuracy of the results obtained by these methods is still controversial.

Method used

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  • Method of determining content of total alkaloid in uncaria macrophylla extract
  • Method of determining content of total alkaloid in uncaria macrophylla extract
  • Method of determining content of total alkaloid in uncaria macrophylla extract

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Effect test

Embodiment 1

[0025] Below is the process that the present inventor carries out experimental research on the content assay method of the total alkaloids of Uncaria macrophyllum in the extract:

[0026] 1. Instruments and reagents

[0027] Waters alliance E2695 high performance liquid chromatograph (American waters company), Inertsil ODS-3C18 chromatographic column (4.6x250mm, 5μm), BS110S 1 / 100,000 electronic analytical balance (Mettler company), 9200S ultrasonic cleaner (Shanghai ).

[0028] Rhynchophylline reference substance (purity ≥ 98%, batch number: 20160518), isorhynchophylline (purity ≥ 98%, batch number: 20160228), Kronosin B (purity ≥ 98%, batch number: 20151123) were all purchased from Baoji City Chenguang Biotechnology Co., Ltd., Ke Nuoxin (purity ≥ 98%, batch number: BCY-0155) was purchased from Jiangxi Herborist Biotechnology Co., Ltd., Uncaria extract (self-made), methanol was chromatographically pure (Fisher, USA), Water was Wahaha purified water, and other reagents were ...

Embodiment 2

[0057] A method for determining the content of total alkaloids in the extract of Uncaria uncariae, the specific method is: determination by high performance liquid chromatography:

[0058] Chromatographic conditions and system suitability experiment: use C18 bonded silica gel as filler; use methanol-0.02% triethylamine=72:28 as mobile phase; flow rate is 1ml min-1; column temperature is 25°C; detection wavelength is 246nm; The number of theoretical plates should not be less than 3000;

[0059] Preparation of the reference substance solution: Accurately weigh the appropriate amount of isorhynchophylline, rhynchophylline, Kronosin B and Kronosin reference substance, put them in a volumetric flask, add methanol to dissolve and set the volume to the mark, and make each 1mL respectively Containing 0.29,0.148,0.13,0.296mg mixed reference solution.

[0060] Preparation of the test solution: Take an appropriate amount of Uncaria total alkaloids sample, place it in a 25mL volumetric f...

Embodiment 3

[0063] A method for determining the content of total alkaloids in the extract of Uncaria uncariae, the specific method is: determination by high performance liquid chromatography:

[0064] Chromatographic conditions and system suitability experiment: use C18 bonded silica gel as filler; use methanol-0.02% triethylamine=70:30 as mobile phase; flow rate is 1ml min-1; column temperature is 30°C; detection wavelength is 246nm; The number of theoretical plates should not be less than 3000;

[0065] Preparation of the reference substance solution: Accurately weigh the appropriate amount of isorhynchophylline, rhynchophylline, Kronosin B and Kronosin reference substance, put them in a volumetric flask, add methanol to dissolve and set the volume to the mark, and make each 1mL respectively Containing 0.29,0.148,0.13,0.296mg mixed reference solution.

[0066] Preparation of the test solution: Take an appropriate amount of Uncaria total alkaloids sample, place it in a 25mL volumetric f...

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Abstract

The invention discloses a method of determining the content of total alkaloid in an uncaria macrophylla extract. The method is high-performance liquid chromatography with isorhynchophylline, rhynchophylline, corynoxine B and corynoxine as contrast and with methanol-0.02% triethylamine=55-75 to 25-45 as a mobile phase. The total alkaloid in the uncaria macrophylla extract is selected as an index for content determination, and the determining method has the advantages that the precision is high, the reproducibility is good, the stability is good, the determined result has high accuracy, the quality of the uncaria macrophylla extract can be effectively controlled, and the clinical use is ensured to be safe and effective.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicine extracts, in particular to a method for determining the content of total alkaloids in the extract of Uncaria uncariae. Background technique [0002] Uncaria macrophylla Wall. is a plant of the Rubiaceae Uncaria genus, and its medicinal parts are stems with hooks, which have the effects of relieving wind and convulsions, clearing heat and calming the liver. It is often used for liver wind internal movement, convulsions, epileptic convulsions, febrile convulsions, cold convulsions, infantile convulsions, pregnancy eclampsia, headache and dizziness. In the well-known large varieties of Chinese patent medicines Tianma Uncaria Granules, Zhengtian Pills, and Pediatric Seven Star Tea, Uncaria large-leaf is used as the main drug. [0003] According to literature reports, alkaloids are the main medicinal components of Uncaria uncaria. At present, there are mainly titration...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/047
Inventor 杨海船李嘉张赟赟张颖陈锋何春欢李红
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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