Method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules
A technology of Pediatric Seven Star Tea and isorhynchophylline, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of few detection methods, complex components, and poor separation of Uncaria alkaloids, and achieve good economic and social benefits , simple pre-treatment, good separation effect
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Embodiment 1
[0058] 1. Determination method of content: Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2015 edition general rule 0512).
[0059] See Table 2 and 3 for medicines and instruments
[0060] Table 2: Instruments used in this experiment
[0061]
[0062] Table 3: Reagents used in this experiment
[0063]
[0064] 2. The chromatographic conditions and system adaptability test for the determination of rhynchophylline and isorhynchophylline in Xiaoerqixingcha granules by HPLC are as follows.
[0065] (1) Preparation of mixed reference solution
[0066] Weigh an appropriate amount of rhynchophylline (purity greater than 99.0%, Shanghai Tongtian Biotechnology Co., Ltd.) and isorhynchophylline (purity greater than 99.0%, China Institute for Food and Drug Control), accurately weigh, add methanol to prepare Uncaria Alkaline and isorhynchophylline mixed control solution.
[0067] (2) preparation of need testing solution
[0068] Accurat...
Embodiment 2
[0114] (1) Preparation of mixed reference solution
[0115]Weigh an appropriate amount of rhynchophylline (purity greater than 99.0%, Shanghai Tongtian Biotechnology Co., Ltd.) and isorhynchophylline (purity greater than 99.0%, China Institute for Food and Drug Control), accurately weigh, add methanol to prepare Uncaria Alkaline and isorhynchophylline mixed control solution.
[0116] (2) preparation of need testing solution
[0117] Accurately weigh 28g of Pediatric Seven Star Tea granules, put them in a conical flask with a stopper, add 84mL of hot water at 70°C to dissolve, ultrasonicate for 30min, let cool, adjust the pH value to 9-10 with ammonia water, and then use 84mL of ethyl acetate Shake and extract once, combine and recover the ethyl acetate solution, add 1 mL of methanol to the residue to dissolve, and filter through a 0.45 μm microporous membrane to obtain the test solution.
[0118] (3) Determination by high performance liquid chromatography
[0119] Precisely...
Embodiment 3
[0129] (1) Preparation of mixed reference solution
[0130] Weigh an appropriate amount of rhynchophylline (purity greater than 99.0%, Shanghai Tongtian Biotechnology Co., Ltd.) and isorhynchophylline (purity greater than 99.0%, China Institute for Food and Drug Control), accurately weigh, add methanol to prepare Uncaria Alkaline and isorhynchophylline mixed control solution.
[0131] (2) preparation of need testing solution
[0132] Accurately weigh 28g of Pediatric Seven Star Tea granules, place them in a conical flask with a stopper, add 140mL of 80°C hot water to dissolve, ultrasonicate for 90min, let cool, adjust the pH value to 9-10 with ammonia water, and then use 140mL of ethyl acetate Shake and extract 3 times, combine and recover the ethyl acetate solution, add 1 mL of methanol to the residue to dissolve, and filter through a 0.45 μm microporous membrane to obtain the test solution.
[0133] (3) Determination by high performance liquid chromatography
[0134] Prec...
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