The present invention relates to a method for real-time measurement of the secretion of at least one compound by at least one individual cell, comprising:the culturing, in a liquid medium, of at least one cell in a culture chamber, at least one wall of which comprises at least one sensitive area, a sensitive area comprising a plurality of ligands, attached to a solid support, each ligand being able to bind specifically to the compound, and an element for real-time transduction of a signal produced by the binding of the compound to one of the ligands;the identification, in a sensitive area, of at least one spot producing a signal;the real-time measurement of the signal produced by the spot identified, representing the amount of compound secreted by an individual cell.