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Arthrobacter sp SCUEC3 strain as well as screening method and application thereof

An Arthrobacter and bacterial strain technology, applied in the field of nicotine degradation, can solve the problem of unproven tobacco leaves in the field, and achieve the effects of accelerated maturation and strong degradation ability.

Inactive Publication Date: 2015-01-07
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0004] Chinese invention patent applications (application numbers: 200710013068.3, 200710013069.8, 200710070805.3, 200910097536.9, etc.), the bacterial strains involved in these patents have effective degradation performance on nicotine, and the only disadvantage is that nicotine degradation is carried out under indoor laboratory conditions. Whether real field tobacco leaves have any effect has not been confirmed experimentally

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  • Arthrobacter sp SCUEC3 strain as well as screening method and application thereof
  • Arthrobacter sp SCUEC3 strain as well as screening method and application thereof

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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0016] The Arthrobacter sp SCUEC3 strain provided in this example was deposited in the China Center for Type Culture Collection on September 9, 2014. The address is: Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M2014402, the 16S nucleotide sequence of the strain is shown in SEQ ID NO.1, including 1177 bases.

[0017] The screening and isolation method of the Arthrobacter sp SCUEC3 (Arthrobacter sp SCUEC3) strain provided in this example is as follows: (1), enrichment culture: put 2 g of the soil in the tobacco planting field into 20 mL of nicotine medium, and incubate at 30° C. Use a 180rpm shaker for cultivation. After culturing for 48 hours, transfer 1 mL of the enrichment solution to fresh nicotine medium to continue the c...

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Abstract

The invention provides an arthrobacter sp SCUEC3 strain. The preservation number of the strain is CCTCC NO: M2014402. A nucleotide sequence of the strain is shown as SEQ ID NO.1. The strain can be applied to nicotine degradation and can be used for accelerating maturing of tobacco leaves. Meanwhile, the invention also provides a method for screening and separating the strain. The method comprises the following steps: firstly, performing enrichment culture; secondly, performing separation and purification; finally, inoculating the separated and purified strain in a nicotine culture medium according to a single colony, performing shake cultivation at room temperature for 1-3 days, determining the content of nicotine in the fermentation liquor of the nicotine culture medium, and re-screening the strain according to the size of the nicotine degradation rate to obtain the arthrobacter sp SCUEC3 strain.

Description

technical field [0001] The invention relates to a microbial strain for degrading nicotine, in particular to an Arthrobacter SCUEC3 strain, a screening method and application of the strain, and belongs to the technical field of nicotine degradation. Background technique [0002] Nicotine is not only the most important chemical component present in tobacco, but the level of nicotine content plays a very important role in the quality of tobacco products. At present, the nicotine content of flue-cured tobacco in my country is generally high, especially in the upper tobacco leaves. For example, the appearance quality of tobacco leaves in some areas around Shennongjia is close to the domestic and international advanced level, but the nicotine content of tobacco leaves in some tobacco-producing counties is as high as 3.5% to 4.5% %, and the nicotine content in the upper part of Burley tobacco is as high as 6%. This not only affects the quality of the upper tobacco, but also affects...

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/06
CPCA24B15/20C12N1/20C12N1/205C12R2001/06
Inventor 李晓华孔文郭利李阳杨振飞王晓丽何冬兰程国军刘涛李开曹丽君陈涛龚传清李永辉曹祥练胡健康卢红良彭功银
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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