Ochrobactrum intermedium SCUEC4 strain, screening method and uses thereof

Abstract

The present invention provides an Ochrobactrum intermedium SCUEC4 strain, wherein the preservation number is CCTCC NO:M2014403, the nucleotide sequence of the strain is represented by SEQ ID NO.1, and the strain can be applied in nicotine degradation and can further be provided for accelerating tobacco leaf maturation. The present invention further provides a method for screening and separating the strain, wherein the method comprises: carrying out enrichment culture, carrying out separation purification, inoculating the separated and purified single colony into a nicotine culture medium, carrying out shaking culture for 1-3 days at a room temperature, determining the nicotine content in the fermentation broth of the nicotine culture medium, and re-screening the strain according to the nicotine degradation rate so as to obtain the Ochrobactrum intermedium SCUEC4 strain.

Description

technical field [0001] The invention relates to a microbial strain for degrading nicotine, in particular to a P. intermedia SCUEC4 strain, a screening method and application of the strain, and belongs to the technical field of nicotine degradation. Background technique [0002] Nicotine is not only the most important chemical component present in tobacco, but the level of nicotine content plays a very important role in the quality of tobacco products. At present, the nicotine content of flue-cured tobacco in my country is generally high, especially in the upper tobacco leaves. For example, the appearance quality of tobacco leaves in some areas around Shennongjia is close to the domestic and international advanced level, but the nicotine content of tobacco leaves in some tobacco-producing counties is as high as 3.5% to 4.5% %, and the nicotine content in the upper part of Burley tobacco is as high as 6%. This not only affects the quality of the upper tobacco, but also affects...

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Problems solved by technology

[0004] Chinese invention patent applications (application numbers: 200710013068.3, 200710013069.8, 200710070805.3, 200910097536.9, etc.), the bacterial strains involved in these patents have effective degradation performance on nicotine, and the only disadvantage is that nicotine degradation is carried out under indoor laboratory conditions. Whether real field tobacco leaves have any effect has not been confirmed experimentally

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