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Nicotine-degrading bacterium and application thereof

A nicotine-degrading bacteria and nicotine-degrading technology can be applied to bacteria, organic fertilizers, microorganisms, etc., and can solve the problems of lowering the quality of compost products.

Inactive Publication Date: 2015-06-24
湖北省烟草公司恩施州公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art that microorganisms are inhibited by high-concentration nicotine in tobacco stalks during the composting process of tobacco stalks, delay the maturity process of tobacco stalks, and reduce the quality of compost products. quality

Method used

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  • Nicotine-degrading bacterium and application thereof
  • Nicotine-degrading bacterium and application thereof
  • Nicotine-degrading bacterium and application thereof

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Embodiment 1

[0030] Embodiment 1: A nicotine-degrading bacterium of the present invention, which is named Arthrobacter histidinolovorans EA-17 taxonomically, and was preserved in the China Type Culture Collection Center on September 15, 2013. The number is CCTCCM2013405. The industrial production of nicotine-degrading bacteria EA-17 is secondary fermentation. The production method is as follows: inoculate the activated strains in LB liquid medium, cultivate them at 30-37°C for 10-18 hours, and prepare first-grade seeds; 0.1%-0.5% of the inoculum was inoculated in 2M liquid LB medium 3 In the fermenter, the liquid volume of the fermenter is 60%, 30-37°C, the stirring speed is 250rpm, the aeration ratio is 0.2VVM-1VVM, the fermentation is 16-24h, and the biomass reaches 3×10 9 CFU / mL.

[0031] An application of nicotine-degrading bacteria in tobacco stalk piles. The use of nicotine-degrading bacteria EA-17 is as follows: after mixing the fermentation liquid of nicotine-degrading bacteria a...

Embodiment 2

[0032] Embodiment 2: Preparation of a kind of nicotine degrading bacteria EA-17 bacterial liquid of the present invention

[0033] 1 medium

[0034] LB medium: peptone 10g, yeast powder 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-pH7.2, sterilized at 121°C for 30min.

[0035] 2 Bacteria preparation

[0036] 2.1 Strain activation: Nicotine-degrading bacteria EA-17 was inoculated on LB medium (2% agar was added on the basis of LB medium), and cultured at 30°C for 18-24h.

[0037] 2.2 Cultivation of first-class strains: Transfer the above-mentioned cultured nicotine-degrading bacteria EA-17 into liquid LB medium, fill in 50ml / 500ml Erlenmeyer flask, culture at 30°C, shaker speed 180r / min, shake Bed culture for 12h.

[0038] 2.3 Fermentation tank culture: the volume of the fermentation tank is 2M 3 , 1200L culture medium, the above-mentioned primary seed inoculum volume is 2.4L, the stirring speed is 250rpm, the aeration ratio is 0.5VVM, the fermentation t...

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Abstract

The invention relates to a nicotine-degrading bacterium and an application thereof. The nicotine-degrading bacterium is characterized in that the bacterium is named as Arthrobacter histidinolovorans EA-17 according to taxonomy and was collected in China Center for Type Culture Collection on September 15, 2013, and the collection number is CCTCC No. M2013405. A strain is fermented by a 2M3 fermentation tank for production, then mixed with a self-made decomposition agent according to the mass ratio of 1:10, further inoculated into a tobacco straw compost according to the inoculation amount of 1% and fermented for 30-45d, and then straw can be decomposed; compared with traditional compost fermentation, the nicotine degradation rate is improved by 60%-78%, the total nitrogen content is increased by 7%-9%, the total phosphorus content is increased by 0.3%-0.6%, and the total potassium content is increased by 6%-8%.

Description

technical field [0001] The invention relates to a nicotine degrading bacterium and application thereof. Background technique [0002] In the production of tobacco planting, there is a large amount of tobacco stalks, which are agricultural wastes. Tobacco stalks contain high concentrations of nicotine, a vicious pollutant, and carry pathogenic bacteria and insect eggs. Discarding or burning a large amount of tobacco stalks will cause serious soil and air pollution and aggravate the spread of soil-borne diseases. Therefore, reasonable treatment of tobacco stalks and the utilization of waste tobacco stalks as resources are the basis for the healthy development of tobacco planting. Tobacco stalks are rich in nutrients and organic matter required by plants such as N, P, and K. Through high-temperature composting technology and microbial degradation, tobacco stalks can be transformed into high-quality organic fertilizers. Moreover, the high-temperature environment of high-temper...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/06
Inventor 陈守文呙亚屏王瑞冀志霞杨亮秦兴成伍良伟徐迪红施河丽
Owner 湖北省烟草公司恩施州公司
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