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Method of nicotine biological degradation

A technology of biodegradation and nicotine, applied in tobacco, food science, tobacco processing, etc., can solve problems such as nicotine pollution, achieve the effects of protecting the environment, saving production costs, and maintaining human health

Inactive Publication Date: 2008-10-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By reducing the content of nicotine in tobacco leaves or tobacco extracts, it can not only solve the problem of industrial availability of upper tobacco leaves in my country, but also solve the problem of environmental pollution caused by nicotine in tobacco waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Inoculate Ochrobactrum intermedium DN2 into common medium (g / L): tryptone 11.34, beef extract 3.00, NaCl 5.00, MgSO 4 ·7H 2 O 3.71, pH value 7.23, 32°C, 120r / min shaker culture for 34h, 10000r / min centrifugation for 10min, collect the bacteria, use PBS (50mM, pH7.0) to prepare the bacteria suspension from the above bacteria, at 15g / L The amount of inoculum was added to the tobacco extract (tobacco waste and water were mixed at a ratio of 1:10, overnight, and the pH value of the water extract was adjusted to 7.0 with 4N NaOH or 1N HCl), at pH 6.5 to 8.5, Stir and react at 28-40°C for 2-4 days, and the degradation rate of nicotine is 85-98%.

Embodiment 2

[0031] After Ochrobactrum intermedium DN2 is expanded and cultivated through ordinary culture medium (the method is the same as in Example 1), centrifuge at 10000r / min for 10min, collect the thalline, prepare the bacterium suspension with the above-mentioned thalline with PBS (50mM, pH7.0), and inoculate with 10% The amount is added to the re-cured tobacco leaves with a moisture content of 60-85%, solid fermentation at 28-40°C, and shaking every 6h for ventilation. The degradation rate of nicotine from 2 to 4 days was 40 to 48%.

Embodiment 3

[0033] After Ochrobactrum intermedium DN2 is expanded and cultivated through ordinary medium (the method is the same as in Example 1), centrifuge at 10000r / min for 10min, collect the thalline, prepare the bacterium suspension with the above-mentioned thalline with PBS (50mM, pH7.0), and inoculate with 18% The amount is inserted into shredded tobacco with a water content of 60-85%, solid fermentation is carried out at 28-40°C, and ventilation is stirred every 6 hours. The degradation rate of nicotine in 2-4 days was 40-56%.

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Abstract

The invention relates to a method of biology degradation nicotine which uses (Ochrobactrum intermedium) DN2 as bacteria seed and uses the nicotine zymosis enzyme of the Ochrobactrum intermedium DN2 or Ochrobactrum intermedium DN2 to effect on the flue-cured tobacco leaf, pipe tobacco, industrial or agricultural tobacco leftover bits and pieces and tobacco residues and the extracting liquid of the above materials to do degradation to part of or all of the nicotine.

Description

technical field [0001] The invention relates to a method for degrading nicotine by using nicotine-degrading bacteria (Ochrobactrum intermedium) DN2, which belongs to a production method for biodegrading nicotine, and is specially used for biodegrading nicotine. Background technique [0002] Nicotine is an alkaloid in common tobacco, commonly known as nicotine (nicotine), its main function lies in the physiological strength it produces, that is, the so-called strength, which is proportional to the nicotine content. Nicotine has a strong stimulating and paralyzing effect on the central nervous system of people. A small amount can excite people, and a large amount can cause dizziness, vomiting and even poisoning death. Adults can be fatal if they ingest 40mg-60mg of nicotine at a time. Therefore, if the nicotine content in tobacco is too high, it will seriously endanger the health of smokers. [0003] The tobacco leaves produced in some tobacco areas in my country, especially ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/20
Inventor 陆兆新袁勇军吕凤霞别小妹李颖
Owner NANJING AGRICULTURAL UNIVERSITY
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