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Tobacco leaf extract culture medium and application thereof

A technology of extraction liquid and medium, applied in the field of microorganisms, can solve the problems of high cost, complicated preparation, and expensive nicotine, and achieve the effects of low cost, convenient use, and simple preparation

Inactive Publication Date: 2010-10-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The currently reported culture media for nicotine-degrading strains have complex components, such as adding Na 2 HPO 4 、KH 2 PO 4 、K 2 SO 4 , MgCl 2 ·6H 2 O, MnCl 2 4H 2 O, FeCl 3 ·6H 2 O, CaCl 2 After waiting for the basic ion components, adding a certain amount of nicotine is not only cumbersome to prepare, but also nicotine is expensive and expensive, so it is urgent to find a simple and cheap alternative medium formula

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0023] The present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0024] The reagents described in the examples are all commercially available products, and the tobacco leaf fragments are purchased from Shandong Jinan General Group. The operation methods are general operation methods in the field unless otherwise specified.

Embodiment 1

[0026] Preparation of tobacco leaf extract:

[0027] Mix the tobacco leaf fragments and water according to the mass volume ratio (g / L) of 20:1 and 80:1, boil for 30 minutes, remove the precipitate by suction filtration, add water to make the volume to 1L, and prepare 2% tobacco leaf extract respectively and 8% tobacco leaf extract; after testing, 2% tobacco leaf extract contains nicotine 2.2g / L, and 8% tobacco leaf extract contains nicotine 9.5g / L.

[0028] (1) Preparation of tobacco leaf extract medium

[0029] ①The components of the strain activation medium (bacteria): 1L of 2% tobacco leaf extract, 3g of beef extract, 10g of peptone, 5g of NaCl, 20g of agar;

[0030] Components of the strain activation medium (fungus): 1 L of 2% tobacco leaf extract, 3 g of glucose, 5 g of peptone, 3 g of yeast powder, and 20 g of agar.

[0031] ② Components of the seed culture solution: 1 L of 2% tobacco leaf extract, 0.5 g of yeast powder.

[0032] ③ Components of the fermentation medi...

Embodiment 2

[0092] Cultivation of Aspergillus oryzae CGMCC NO.3687 with Nicotine Degradation Ability Using Tobacco Leaf Extract Medium

[0093] The preparation of the strain activation medium (fungus), seed culture solution and fermentation medium in this embodiment is the same as that in Example 1.

[0094](1) Select Aspergillus oryzae CGMCC NO.3687 and inoculate it into the strain activation medium (fungi) to activate for 40 hours. The culture temperature is 28° C. Then, take the activated Aspergillus oryzae CGMCC NO.3687 and inoculate it into the seed culture medium, and cultivate it for 30 hours. hour, the culture temperature is 28 DEG C to obtain the primary culture fluid; the primary culture fluid is transferred to the fermentation medium with an inoculum size of 5% (v / v) by volume, the culture temperature is 28 DEG C, and the shaking table speed is 150 rpm. After culturing for 40 hours, the mycelium was collected by suction filtration, the growth of the bacteria was measured, and ...

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PUM

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Abstract

The invention relates to a culture medium, in particular to a tobacco leaf extract culture medium for cultivating nicotine degradation microorganisms and application thereof and belongs to the technical field of microorganisms. The tobacco leaf extract culture medium provided by the invention mainly comprises tobacco leaf extract. A method for preparing the culture medium comprises the following steps of: mixing tobacco leaf chips and water in a mass volume ratio of 10-80:1; boiling the mixture for 25 to 35 minutes; and performing extraction filtration on the boiled mixture to achieve constant volume so as to obtain the tobacco leaf extract. The invention also provides the application of the tobacco leaf extract culture medium in the aspect of cultivating the nicotine degradation microorganisms. The culture medium is suitable for cultivating various nicotine degradation microorganisms and can obtain high biomass and improve nicotine degradation capability. The culture medium has the advantages of simple preparation, convenient use, low cost and suitability for industrial application.

Description

technical field [0001] The invention relates to a culture medium, in particular to a tobacco leaf extract culture medium for cultivating nicotine-degrading microorganisms and an application thereof, belonging to the technical field of microorganisms. technical background [0002] Nicotine is an alkaloid in tobacco. It has a strong stimulating and paralyzing effect on the central nervous system. A small amount can excite people, but a large amount can cause dizziness, vomiting and even poisoning death. Excessive nicotine content in tobacco leaves will seriously endanger the health of smokers. In addition, nicotine is also an environmentally toxic substance. During the production and processing of tobacco products, a large amount of solid, liquid and gas waste will be produced. If these wastes are released into the environment without treatment, they will seriously damage the ecological environment and endanger the environment. human health. Microbial degradation of nicotine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/24C12N1/20C12R1/69C12R1/38C12R1/11C12R1/01
Inventor 肖敏孟祥璟卢丽丽
Owner SHANDONG UNIV
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