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Quorum-sensing signal molecular preparation and application thereof in tobacco waste treatment

A quorum sensing signal and tobacco waste technology, applied in the field of environmental biology, can solve the problems of unknown contribution to total COD removal of tobacco waste, increased maintenance costs, unfavorable promotion and application, etc., to improve comprehensive treatment efficiency, increase strain types and quantities , cost reduction effect

Inactive Publication Date: 2012-03-28
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these existing technologies have good effects on the removal of nicotine in tobacco, in practical applications, since these pure bacteria agents have not yet persisted in the polluted environment and reproduced stably, they need to be frequently added to the polluted environment. These pure bacterial agents or crude enzyme agents not only increase the cost and maintenance costs; more importantly, whether these pure bacterial agents can preferentially use nicotine as a carbon and nitrogen source in rich nutrient sources, and have a significant impact on the total COD of tobacco waste. Eliminate issues such as unknown contribution, which is not conducive to popularization and application

Method used

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  • Quorum-sensing signal molecular preparation and application thereof in tobacco waste treatment
  • Quorum-sensing signal molecular preparation and application thereof in tobacco waste treatment
  • Quorum-sensing signal molecular preparation and application thereof in tobacco waste treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Treatment of Tobacco Waste Using Pseudomonas HF-1 and Quorum Sensing Signaling Molecule Preparations

[0027] (1) Screening and cultivation of Pseudomonas HF-1:

[0028] Inoculate 5g of tobacco-contaminated soil into sterilized nicotine inorganic salt medium containing 50mL of nicotine with a concentration of 1g / L, and incubate at a constant temperature at 28°C; after 7 days, take 1mL of the above culture solution and inoculate it in the same medium, and continue to maintain constant temperature Cultivate for 7 days; transfer 4 times in succession to enrich nicotine-degrading bacteria. Dilute and inoculate the enriched culture obtained on the nicotine inorganic salt solid medium, and culture at a constant temperature of 28°C for 2-3 days, pick different single colonies, continue to dilute and spread on the above-mentioned plate for constant temperature culture; so continuously for 3 Once, a pure nicotine-degrading strain was obtained.

[0029] After identifi...

Embodiment 2

[0043] Example 2 Treatment of Tobacco Waste Using Arthrobacter TW and Quorum Sensing Signaling Molecule Preparations

[0044] (1) Screening and cultivation of Arthrobacter TW:

[0045] Tobacco waste from Hangzhou Liqun Cigarette Factory was taken and continuously cultured for 14 days to enrich nicotine-degrading bacteria; the enriched culture was diluted and inoculated on nicotine inorganic salt medium for culture; colonies were picked and continued to be diluted and cultured. until a single colony was picked.

[0046] After identification, the single colony was Arthrobacter sp. (Acinetobacter sp.), named Arthrobacter TW. The strain is a Gram-negative bacterium with a size of (0.5-0.7×1.6-2.0 μm), and the colony of Arthrobacter TW is white and moist round; the GenBank accession number of the 16S rDNA sequence of the bacterium is FJ753401. Wang et al. (Wang Meizhen, Yang Guiqin, Wang Xin, Yao Yanlai, Min Hang, Lv Zhenmei. Nicotine degradation by two novel bacterial isolates o...

Embodiment 3

[0055] Example 3 Treatment of Tobacco Waste Using Sphingomonas TY and Quorum Sensing Signaling Molecule Preparation

[0056] (1) Screening and cultivation of Sphingomonas TY:

[0057] Tobacco waste from Hangzhou Liqun Cigarette Factory was taken and continuously cultured for 14 days to enrich nicotine-degrading bacteria; the enriched culture was diluted and inoculated on nicotine inorganic salt medium for culture; colonies were picked and continued to be diluted and cultured. until a single colony was picked.

[0058] After identification, the single colony was Sphingomonas sp., named Sphingomonas TY. The strain is a Gram-negative bacterium with a size of (0.4-0.6×1.6-1.8 μm), and the colonies are light yellow and moist round; the GenBank accession number of the 16S rDNA sequence of the bacterium is FJ754274. Wang et al. (Wang Meizhen, Yang Guiqin, Wang Xin, Yao Yanlai, Min Hang, Lv Zhenmei. Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sph...

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Abstract

The present invention discloses a quorum-sensing signal molecular preparation, which is prepared through the following steps: culturing alpha-proteobacterial, delta-proteobacterial and flavobacteria microorganisms respectively; taking culture solution or supernate made by centrifugalizing culture solution, and mixing with the volume proportion of 1-4:1:1-2; and cooling and drying. The quorum-sensing signal molecular preparation can precipitate nicotine degrading bacteria to colonize and constantly multiply in the contaminated environment, and can precipitate microbial populations in the environment to dynamically develop, so as to effectively improve nicotine removal and the comprehensive treatment efficiency of contaminants. The preparation can treat tobacco waste in cooperation with nicotine degrading bacteria and has the advantage of lasting action with one time of feeding. The preparation can greatly reduce the starting time for an active sludge reactor and accelerate the stabilization of the reactor. The operation is convenient, and both the cost and the maintenance expense can be reduced greatly. Therefore, the present invention provides an ideal treatment method for tobacco waste.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a quorum sensing signal molecule preparation and its application in treating tobacco waste. Background technique [0002] Tobacco is one of the most important profit and tax industries in my country, and the waste generated by the tobacco industry is huge. my country produces about 1.3-1.7 million tons of tobacco waste every year, and the average content of nicotine in dry waste is as high as 18g / kg, which is 36 times higher than the European Union's control standard for nicotine content in toxic and harmful tobacco waste; tens of millions of tons of tobacco processing wastewater are generated , and its nicotine content is also as high as 1.0g / L. Nicotine is the main alkaloid in tobacco and the main environmental pollutant in tobacco waste. It is soluble in water and a variety of organic solvents, and can pass through complex biofilm systems such as the blood-brain barr...

Claims

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Application Information

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IPC IPC(8): C12P1/04A62D3/02C12R1/01C12R1/20A62D101/28
Inventor 汪美贞何虹蓁冯华军郑昕沈东升吕镇梅闵航
Owner ZHEJIANG GONGSHANG UNIVERSITY
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