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Pseudomonas putida strain used for nicotine degradation and isolation and identification method and application thereof

A technology for degrading Pseudomonas putida and nicotine, applied in the biological field, can solve problems such as the impact of cigarette quality, and achieve the effects of high biological activity, reduction of nicotine in tobacco leaves, and stable genetic properties

Inactive Publication Date: 2017-11-24
HONGYUN HONGHE TOBACCO (GRP) CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although there are some methods to reduce the nicotine content in tobacco, such as extraction and agricultural measures, these technologies will also affect the quality of cigarettes while reducing nicotine

Method used

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  • Pseudomonas putida strain used for nicotine degradation and isolation and identification method and application thereof
  • Pseudomonas putida strain used for nicotine degradation and isolation and identification method and application thereof
  • Pseudomonas putida strain used for nicotine degradation and isolation and identification method and application thereof

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example

[0044] Example: Nicotine Degradation Assay

[0045] 30 μL ND9 strain cell suspension (OD 600 0.25‐0.40) were inoculated into 3 mL basal medium for culture. Samples were taken regularly to measure the growth of bacteria and the degradation of nicotine, and each experiment was repeated 3 times.

[0046] Cultivation temperature: the concentration of nicotine is 1.0g / L, the temperature is 20, 25, 28, 30 and 37°C, pH 7.0, 150rpm cultivation.

[0047] Initial pH of the culture medium: the initial pHs were 5, 7, 9 and 10 respectively, the nicotine concentration was 1.0 g / L, and cultured at 28° C. and 150 rpm.

[0048] Nicotine initial concentration: nicotine concentrations were 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 g / L, cultured at pH 7.0, 28°C, 150rpm.

[0049] use OD 600 Value and nicotine concentration (g / L) to compare the rate of nicotine degradation of ND9 strain under different culture conditions such as pH, temperature and nicotine concentration. Under the conditions of 1.0g / L ...

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Abstract

The invention discloses a pseudomonas putida strain used for nicotine degradation with the preservation number being CGMCC NO.13055. The strain is obtained from a tobacco field soil sample and used for degrading nicotine. The invention further discloses an isolation and identification method of the pseudomonas putida strain used for nicotine degradation. The pseudomonas putida strain used for nicotine degradation has the beneficial effects that the strain has high biological activity, and is easy to culture and stable in genetic property, the nicotine degradation rate is high, the pseudomonas putida strain can be used for degrading the nicotine in tobacco or tobacco waste, and the nicotine in tobacco leaves is reduced.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a strain of Pseudomonas putida used for nicotine degradation and its isolation and identification method and application. Background technique [0002] Nicotine, commonly known as nicotine, is an alkaloid in common tobacco and an important component of tobacco. Its function mainly lies in the physiological strength it produces, which is commonly referred to as strength, and the strength is directly proportional to the nicotine content. Nicotine can make people addicted or dependent, and affect people's health. It has been reported that nicotine not only increases heart rate and blood pressure, but also causes vascular endothelial dysfunction. In 2000, there were approximately 4.9 million smoking-related sudden deaths worldwide. Smoking is responsible for at least 30% of cancer deaths and 87% of lung cancer deaths. Therefore, excessive nicotine content in tobacco will serio...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12Q1/68C12Q1/04A24B3/18C12R1/40
CPCA24B3/18C12N1/02C12Q1/689C12N1/205C12R2001/40
Inventor 李伟张静杨金广王杰王超刘浩李云贵肖志新申莉莉王凤龙李莹董石飞唐鹭聂鑫
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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