39 results about "Nicotine concentration" patented technology

The disclosure relates to an improved method of enhancing nicotine concentrations in a gaseous carrier. The methods are adaptable to the delivery of nicotine for therapeutic effect in various diseases, in particular nicotine for tobacco product use cessation, substitution and / or harm reduction. The disclosure further relates various devices and device design principles for practicing these methods.

A method for making a nicotine-containing toothpick, includes creating a solution having a predetermined concentration of nicotine. A toothpick is exposed to the solution, and the amount of solution added to the toothpick is determined. The solution may be a liquid solution, or a saliva soluble powder solution. The concentration of the solution can be adjusted, or multiple solutions of varying concentrations of nicotine made, to create toothpicks of varying nicotine levels. Sweeteners, medicaments, breath fresheners, or dentifrices can be added to the toothpick.

The disclosure relates to an improved method of enhancing nicotine concentrations in a gaseous carrier. The methods are adaptable to the delivery of nicotine for therapeutic effect in various diseases, in particular nicotine for tobacco product use cessation, substitution and / or harm reduction. The disclosure further relates various devices and device design principles for practicing these methods.

The invention discloses an Acinetobacter and application thereof. Production bacterial strain liquid is fermented and cultured to form corresponding preparation. The Production bacterial strain is the Acinetobacter (Acinetobacter sp.ND12); and the bacterial strain was preserved in China General Microbiological Culture Collection Center on November 5, 2009, and a preservation number is CGMCC No.3410. The bacterial strain has stronger nicotine degradation capacity and nicotine toxicity resistance capacity, and can be grown at the nicotine concentration of 3.5g / L; and by large-scale culturing of the bacterial strain, the pure nicotine can be degraded by using a whole cell of the bacterial strain as a biocatalyst, and meanwhile the content of the nicotine in tobacco is also reduced. The Acinetobacter and the application thereof have the advantages of capacities of adjusting the content of the nicotine in the tobacco raw material, improving availability of the tobacco, degrading the nicotine in smoke dust, reducing pollution of the nicotine to environment and the like.

A method for predicting nicotine replacement dosage to achieve a target nicotine serum concentration relies on measuring blood nicotine concentration prior to smoking cessation. At least two values corresponding to other patient characteristics, such as body mass, cumulative smoking, psychological dependence, age, and menopausal status, are also determined and used to predict expected blood nicotine concentrations based on nicotine replacement dosages. Such methods are useful in achieving target blood nicotine concentrations for smoking cessation and therapy.

A system is disclosed which makes it possible for a patient to be delivered gradually reduced amounts of nicotine over time thereby allowing the patient to be gradually weaned off of dependence on nicotine and quit smoking. The system is comprised of a means for aerosolizing a formulation and containers of formulation. The formulation is comprised of nicotine in a pharmaceutically acceptable carrier. Preferably, a plurality of containers are produced wherein the concentration of nicotine in the different containers or different groups of containers is reduced. The patient uses containers with the highest concentration initially and gradually moves towards using containers with lower and lower concentrations of nicotine until the patient's dependence on nicotine is eliminated.

The invention discloses a pseudomonas putida to metabolite nicotine, which is characterized by the following: preserving in China Typical Culture Preservation Center (Wuhan university, china Wuhan) with keeping number as CCTCC NO.M 206131; possessing stronger nicotine metabolic ability and nicotine toxin immunity ability; growing highest at 5g / L nicotine density; culturing a great deal of a strain; using intact cell as biological activator; co-suspending in phosphoric acid buffer with solid waste matter from tobacco industry; oscillating; degrading nicotine completely. This bacterium possesses simple operation, which can be used to dispose tobacco waste matter widely.

The invention provides a new nicotine-degrading bacteria-pseudomonas ZUTSKD and its application. The ZUTSKD (Pseudomonas sp.ZUTSKD) is stored in China Center for Type Culture Collection, the storing date was June 18th, 2007, the preservation no. is CCTCC NO:M 207083. The bacteria can degrade nicotine and generate such intermediate as 2, 3'-Bipyridine, cotinine, 3-(3, 4-dihydro-2H-pyrrol-5-y1)-Pyridine and 3-Pyridinecarboxylic acid, the max. nicotine concentration withstandable is 5.5 g / L. The invention can reduce the nicotine in waste cigarette residues, and is of certain application prospect in the industry.

The invention discloses a method for manufacturing a middle aroma type buccal cigarette. The method includes the steps that a tobacco extracting solution with certain nicotine concentration and peculiar aroma components is obtained through extraction from middle aroma type flue-cured tobacco leaves; the tobacco extracting solution is added into a candy ground substance which is mainly composed of saccharides and / or protein, fat, colloid, food additives and the like, and a finished product can be obtained through a candy preparation technology. The physiological need of tobacco consumers can be met, the ambient environment cannot be damaged, possible harm to consumers is further reduced, the preference of a specific tobacco consumer group to the aroma of middle aroma type flue-cured tobaccos can be met, mature and diversified candy processing methods are used for overcoming the defects of a traditional buccal cigarette, the needs of different consumer groups can be met, the mouthfeel is more abundant, and the buccal cigarette can be received more easily.

The invention discloses a method for preparing a Fen-flavor mouth-holding cigarette. The method includes the steps of extracting and concentrating tobacco extract of Fen-flavor flue-cured tobacco through a steam distillation method; adding the tobacco extract with the certain nicotine concentration and tobacco unique flavor compositions to a candy substrate, wherein the candy substrate is mainly composed of candy and / or proteins, grease, gel, food additives and the like; preparing the finished product through a candy preparation technology. According to the method, the physical demands of tobacco customers can be met without damaging the surrounding environment, damage possibly caused to the customers is further reduced, preference of specific tobacco customers for flavor of the Fen-flavor flue-cured cigarette can be satisfied, the defects of a traditional mouth-holding cigarette are overcome through mature and diversified candy processing measures, and the cigarette can meet demands of different customers, is richer in flavor and can be more easily accepted.

The invention discloses a pseudomonas putida to metabolite nicotine, which is characterized by the following: preserving in China Typical Culture Preservation Center (Wuhan university, china Wuhan) with keeping number as CCTCC NO.M 205038; possessing stronger nicotine metabolic ability and nicotine toxin immunity ability; growing highest at 6g / L nicotine density; culturing a good deal of bacterial strain; using intact cell as biological activator; degrading nicotine from tobacco industrial waste water completely. This bacterium possesses simple operation and quick disposing waste water speed, which can be used to dispose the waste water from tobacco industrial widely.

The invention discloses a nicotine blended product, a preparation method of the nicotine blended product, and electronic cigarette tar. The nicotine blended product comprises nicotine, organic acid, asolvent and plum refined substance. According to the technical scheme provided by the invention, the concentration of nicotine in the cigarette tar can be effectively reduced, and meanwhile, the smoking satisfaction of a consumer can be satisfied.

The invention discloses a method for detecting nicotine based on fluorescence resonance energy transfer. The method comprises the following steps: (1) fitting of a regression equation: adding a water-soluble metal quantum dot solution and a water-soluble carbon quantum dot solution having certain concentrations and volumes into several nicotine standard solutions having different concentrations, diluting to a certain volume with a weak acid solution, and then respectively measuring fluorescence intensities F and F0 to obtain a regression equation of a relationship between (F-F0) / F0 and a nicotine concentration, wherein F and F0 are respectively the fluorescence intensities of metal quantum dots in the presence and absence of nicotine; (2) sample measurement: measuring F and F0 of a nicotine solution having unknown concentration, and substituting the obtained F and F0 into the regression equation obtained in step (1) to calculate the content of nicotine in samples having unknown concentration. The method provided by the invention utilizes the fluorescence resonance energy transfer between carbon quantum dots and metal quantum dots to propose a novel method for detecting nicotine; the method has advantages of being good in stability, high in sensitivity, good in selectivity, simple in operation method, and green.

The invention provides bacillus amyloliquefaciens S4 for fermentation of a tobacco straw bio-organic fertilizer. The bacillus amyloliquefaciens S4 have a preservation number of CCTCC No: M2010249, are separated and selected from tobacco straws under the conditions of a high temperature and a high nicotine concentration, and can survive in the presence of nicotine. After culture and expanding propagation, the bacillus amyloliquefaciens S4 are added into a waste tobacco straw mixture and the waste tobacco straw mixture with the bacillus amyloliquefaciens S4 is subjected to solid state fermentation so that the waste tobacco straw mixture is transformed into a tobacco straw bio-organic fertilizer. The tobacco straw bio-organic fertilizer can replace other fertilizers to realize tobacco production, can improve a tobacco leaf yield, a tobacco leaf output value and tobacco leaf smoking quality, and can eliminate the pollution produced by tobacco straw waste on the environment.

A dosimetric smoke determination method used in a full-smoke exposure experiment is used for real-time and quantitative determination of smoke dosage in a full-smoke exposure bin through combination of a quartz micro balance wafer and an inserted cell culture dish. The method has the maximum characteristic that an actual microenvironment for smoke exposure is simulated, the actual concentration (including the mass concentration of smoke particulate matter and nicotine concentration) of cigarette smoke in the full-smoke exposure bin is detected in a real-time, sensitive and quantitative mode, the dosage-effect relation of a smoke toxicity effect is accurately represented, and a real smoke dosage basis is provided for evaluation of cigarette smoke in-vitro toxicology. By applying method, the toxicity mechanism of the cigarette smoke can be better interpreted, and a method basis and technical support are provided for health risk assessment and research of tobacco products. In addition, the method is wide in applicability and can be applied to in-vitro toxicity testing and research of cigarettes, electronic cigarettes, cigarettes non-combustible in heating and aerosols different in source, and testing results are good in repeatability, high in stability and strong in sensitivity.

The invention relates to a method for preparing nicotine sulfate, and belongs to the technical field of tobacco chemistry. According to the method, an aquaporin membrane and a polyamide membrane are used as forward osmosis concentration membranes, an inorganic salt is used as an absorbing agent, and a low-concentration nicotine distillate is subjected to forward osmosis concentration until the trapped fluid nicotine concentration is 5%-20%; 5%-40% sulfuric acid which is slightly excessive in chemical calculation is added into a trapped fluid to obtain a nicotine sulfate solution with relatively high concentration, and then evaporating and concentrating are carried out to obtain a 40%-60% nicotine sulfate product. According to the method, the concentration of the reactant nicotine during nicotine sulfate generation is greatly improved by adopting forward osmosis concentration, so that the concentration of the reaction product nicotine sulfate is improved, the evaporation capacity of water during preparation of a final product by subsequent evaporation and concentration is reduced, and the evaporation energy consumption is greatly reduced; by utilizing the characteristic of poor interception efficiency of forward osmosis concentration on ammonia, the ammonia content in the trapped liquid is greatly reduced, so that the ammonium salt content in a final product is greatly reduced,and the purity of nicotine sulfate is greatly improved.

A method of producing a consumable nicotine composition includes blending a measured quantity of nicotine into a solution. A nicotine concentration is achieved in the solution of between 0.0001% and 0.1% and the solution apportioned so that each portion contains a precise quantity of nicotine appropriate for consumption in a single use by a single person. Each portion is introduced into a respective single-serving beverage. Another method of producing a consumable nicotine composition also includes blending a measured quantity of nicotine into a solution and achieving a nicotine concentration in the solution of between 0.0001% and 0.1%. The solution is likewise apportioned so that each portion contains a precise quantity of nicotine appropriate for consumption in a single use by a single person. Each portion is introduced into a respective beverage and each beverage packaged into a separate single-use dispenser.

The invention provides a new strain of nicotine degrading bacteria—Pseudomonas ZUTSKD and its application. Pseudomonas sp. ZUTSKD (Pseudomonas sp. ZUTSKD), preserved in China Center for Type Culture Collection, date of preservation is June 18, 2007, preservation number CCTCCNO: M 207083. The bacteria can degrade nicotine to produce intermediate products such as 2,3'-Bipyridine, cotinine, 3-(3,4-dihydro-2H-pyrrol-5-yl)-Pyridine and 3-Pyridinecarboxylic acid, and can tolerate the highest nicotine With a concentration of 5.5g / L, it can degrade nicotine in waste tobacco powder, and has a certain application prospect in industry.

A composition for administration to the nasal mucosa of a subject comprises a solution of nicotine or a pharmaceutically acceptable salt thereof in a pharmaceutically acceptable solvent. The composition has a nicotine concentration less than 0.08 mg / ml. The composition used alone assists in reduction of the desire of a subject to smoke tobacco. It also reduces the nasal symptoms associated with administration of higher concentrations of nicotine to the nasal mucosa.

The invention discloses a quantitative detection method for nicotine in cell lysate. The quantitative detection method takes the cell lysate as an object to be detected; the cell lysate is prepared by adding a cell lysis solvent into cell sap / whole blood; the quantitative detection method sequentially comprises the following steps: adding a sodium hydroxide solution and ethyl ether into the cell lysate; after drying an obtained ethyl ether layer, re-dissolving and centrifuging to obtain supernatant I; carrying out chromatography treatment on the supernatant I to obtain supernatant II; carrying out high performance liquid chromatography system analysis on the supernatant II; substituting an obtained medicine peak area Y of the nicotine into a formula Y is equal to 74729X-354.61 and converting to obtain the concentration of the nicotine in the cell sap / whole blood to be detected.

A method of producing a consumable nicotine composition includes blending a measured quantity of nicotine into a solution. A nicotine concentration is achieved in the solution of between 0.0001% and 0.1% and the solution apportioned so that each portion contains a precise quantity of nicotine appropriate for consumption in a single use by a single person. Each portion is introduced into a respective single-serving beverage. Another method of producing a consumable nicotine composition also includes blending a measured quantity of nicotine into a solution and achieving a nicotine concentration in the solution of between 0.0001% and 0.1%. The solution is likewise apportioned so that each portion contains a precise quantity of nicotine appropriate for consumption in a single use by a single person. Each portion is introduced into a respective beverage and each beverage packaged into a separate single-use dispenser.

The invention relates to a method for determining nicotine content in a tobacco product. The method comprises the following steps: selecting an organic solvent with a volume of V, mixing the organic solvent with a tobacco product with a mass of m, and extracting nicotine in the tobacco product to obtain an extract liquid, the organic solvent being a triethylamine-containing methanol solvent; carrying out nicotine content analysis on the extracting solution by adopting a gas chromatography method to obtain the nicotine concentration in the extracting solution; and calculating the nicotine content in the tobacco product according to V, m and the obtained nicotine concentration. According to the method, the nicotine in the tobacco product can be completely extracted, so that the nicotine in the tobacco product can be accurately determined. Meanwhile, a one-step extraction method is adopted, so that the operation steps are reduced, the loss of nicotine in the extraction operation is reduced, and the accuracy of nicotine measurement is further improved. The invention also relates to a method for simultaneously determining the content of water, 1, 2-propylene glycol, glycerol and nicotine in tobacco products.

The invention discloses a filter tip trapped nicotine concentration distribution pattern measuring method which comprises the following steps: concentrically cutting a sucked filter tip along the axis after radially cutting with a laser, or radially cutting a sucked filter tip after concentrically cutting along the axis; after finishing cutting, separating the annular filter tip sections from the corresponding number of cylindrical filter tip sections, carrying out quantitative analysis on the nicotine content in the cut and separated filter tip sections; and after calculating the nicotine concentration, carrying out polynomial fitting on filter tip trapped nicotine concentration distribution data, or carrying out polynomial fitting and interpolation analysis to obtain the filter tip trapped nicotine concentration distribution pattern. By measuring the longitudinal, cross section radial and spatial distribution pattern of different filter tip trapped nicotine concentrations, the invention can master the filtration trapped distribution characteristics of nicotine in filter tips in different structures, can quantitatively analyze the nicotine trapping efficiency and contribution of all parts of filter tips in different structures in flue gas, and can be used for evaluation of flue gas nicotine filtering quality of functional filter tips and design improvement of the functional filter tips.

A dosimetric smoke determination method used in a full-smoke exposure experiment is used for real-time and quantitative determination of smoke dosage in a full-smoke exposure bin through combination of a quartz micro balance wafer and an inserted cell culture dish. The method has the maximum characteristic that an actual microenvironment for smoke exposure is simulated, the actual concentration (including the mass concentration of smoke particulate matter and nicotine concentration) of cigarette smoke in the full-smoke exposure bin is detected in a real-time, sensitive and quantitative mode, the dosage-effect relation of a smoke toxicity effect is accurately represented, and a real smoke dosage basis is provided for evaluation of cigarette smoke in-vitro toxicology. By applying method, the toxicity mechanism of the cigarette smoke can be better interpreted, and a method basis and technical support are provided for health risk assessment and research of tobacco products. In addition, the method is wide in applicability and can be applied to in-vitro toxicity testing and research of cigarettes, electronic cigarettes, cigarettes non-combustible in heating and aerosols different in source, and testing results are good in repeatability, high in stability and strong in sensitivity.

The invention discloses a method for manufacturing a middle aroma type buccal cigarette. The method includes the steps that a tobacco extracting solution with certain nicotine concentration and peculiar aroma components is obtained through extraction from middle aroma type flue-cured tobacco leaves; the tobacco extracting solution is added into a candy ground substance which is mainly composed of saccharides and / or protein, fat, colloid, food additives and the like, and a finished product can be obtained through a candy preparation technology. The physiological need of tobacco consumers can be met, the ambient environment cannot be damaged, possible harm to consumers is further reduced, the preference of a specific tobacco consumer group to the aroma of middle aroma type flue-cured tobaccos can be met, mature and diversified candy processing methods are used for overcoming the defects of a traditional buccal cigarette, the needs of different consumer groups can be met, the mouthfeel is more abundant, and the buccal cigarette can be received more easily.

The invention discloses a method for accurately determining total nicotine in electronic cigarette liquid and aerosol containing nicotine salt. According to the method, the content of the total nicotine in the electronic cigarette liquid containing the nicotine salt is measured through a gas phase method (GC-FID), the influence of the temperature of a sample inlet, the split ratio and the nicotine concentration of the sample inlet on the content of the total nicotine is studied in detail, the temperature of the gas phase sample inlet is 250-300 DEG C, the split ratio is 5-50: 1, and the content of the nicotine in the electronic cigarette liquid containing the nicotine salt is measured. When the nicotine concentration of a sample inlet is smaller than or equal to 0.001 mg / mL, the total nicotine content in the electronic cigarette containing the nicotine salt can be accurately measured.

The invention relates to a nicotin-3, 5-dihydroxybenzoate compound crystal and application thereof in smokeless tobacco products, and belongs to the technical field of tobacco chemistry. The molecularformula of the crystal is C17H24N2O6. The crystal belongs to a monoclinic system, the space group is P21, and the cell parameters are as follows: a=8.4365 (6) angstroms, b=13.1486 (11) angstroms, c=8.6019 (5) angstroms, [alpha]=90.00 degrees, [beta]=101.976 (6) degrees, [gamma]=90.00 degrees and V=933.42 (12) angstroms. The invention provides a preparation method of nicotin-3, 5-dione. Accordingto the present invention, the 1, 5-dihydroxybenzoate compound crystal is adopted to replace the nicotine monomer to be used in smokeless tobacco products, such that the irritation of the protonated nicotine on the oral cavity and the respiratory tract is significantly reduced compared to the free nicotine, the high nicotine concentration is achieved in the limited aerosol, and the toxicity of thenicotine on the human body is substantially reduced.