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Acinetobacter and application thereof

A technology of Acinetobacter and bacilli, applied in the field of applied microorganisms, can solve the problems of undiscovered public reports, etc., and achieve the effects of promoting healthy development, easy operation, and simple process

Active Publication Date: 2012-01-25
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But through document retrieval, do not find yet the public report of identical document with the content of the present invention

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  • Acinetobacter and application thereof
  • Acinetobacter and application thereof
  • Acinetobacter and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] ——Degradation test of nicotine in Baoshan Hongda C3F tobacco leaves by Acinetobacter ND12 preparation

[0027] (1) Culture method of Acinetobacter sp.ND12 (Acinetobacter sp.ND12)

[0028] 1) Test tube slant culture

[0029] A Luria-Bertani (LB) medium was used, and the formula was: 10 grams of peptone, 10 grams of sodium chloride, 5 grams of yeast extract, 20 grams of agar, and distilled water to a volume of 1000 milliliters. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Acinetobacter sp.ND12, culture at 28°C for 24 hours, and obtain test tube species.

[0030] 2) Liquid expansion culture

[0031] A. Seed culture (LB medium), the formula is (gram / liter, g / v): 10 grams of peptone, 10 grams of sodium chloride, 5 grams of yeast extract, distilled water to 1000 milliliters. Sterilize at 121°C for 25 minutes. Inoculate Acinetobacter sp.ND12 test tube species, and culture at 28°C for 12 hours to obtain liquid strains.

[0032] B. Fermentation medium,...

Embodiment 2

[0045] ——Acinetobacter preparation for reducing nicotine content in tobacco leaves K326

[0046] 1) Preparation of Acinetobacter preparation: the liquid and solid culture of Acinetobacter, the preparation method of degrading nicotine bacteria preparation is the same as embodiment 1.

[0047] 2) Acinetobacter preparation is used to reduce the nicotine content in tobacco leaves K326

[0048] Weigh 2g of tobacco leaf K326, spray 5ml of bacterial preparation, and place in a constant temperature and humidity incubator at 28°C for 12-24 hours. 90% of the nicotine in the tobacco leaves can be effectively degraded.

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Abstract

The invention discloses an Acinetobacter and application thereof. Production bacterial strain liquid is fermented and cultured to form corresponding preparation. The Production bacterial strain is the Acinetobacter (Acinetobacter sp.ND12); and the bacterial strain was preserved in China General Microbiological Culture Collection Center on November 5, 2009, and a preservation number is CGMCC No.3410. The bacterial strain has stronger nicotine degradation capacity and nicotine toxicity resistance capacity, and can be grown at the nicotine concentration of 3.5g / L; and by large-scale culturing of the bacterial strain, the pure nicotine can be degraded by using a whole cell of the bacterial strain as a biocatalyst, and meanwhile the content of the nicotine in tobacco is also reduced. The Acinetobacter and the application thereof have the advantages of capacities of adjusting the content of the nicotine in the tobacco raw material, improving availability of the tobacco, degrading the nicotine in smoke dust, reducing pollution of the nicotine to environment and the like.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms, and more specifically, the invention relates to an Acinetobacter capable of degrading nicotine. At the same time, it also relates to the new application of the Acinetobacter in tobacco production. Background technique [0002] Tobacco is one of the important pillars of my country's finances. With the gradual deepening of the internationalization of my country's cigarette and tobacco leaf market, the "Framework Convention on Tobacco Control" has stricter restrictions on tobacco products, and China Tobacco is facing new situations and challenges. For this reason, the State Tobacco Monopoly Administration formulated the "Outline of China's Cigarette Science and Technology Development", clearly stating that the direction of China's cigarette development is: market-oriented, vigorously develop Chinese-style cigarettes. The key technology for developing Chinese-style cigarettes is to "r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/01
Inventor 曾晓鹰段焰青王明锋杨金奎黄立斌张克勤黄静文
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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