Bare-glass fly arthrobacter strain and method for producing feeding proteins by using bare-glass fly arthrobacter strain to decompose gossypol

Abstract

The invention discloses a bare-glass fly arthrobacter strain, which has the collection number of CGMCC No.3554 and has 16S rRNA genes shown as ID No:1. A method for producing feeding proteins by using the bare-glass fly arthrobacter strain to decompose gossypol, which comprises the following steps of: a, preparing seeds; and b, inoculating the seeds into a cottonseed cake dreg culture medium, in which glucose and corn steep liquor are added, for growth, and fermenting the seeds for 72 hours at 37 DEG C to prepare the fermented and detoxicated cottonseed cake dreg feeding proteins. A fermentation culture medium of the bare-glass fly arthrobacter strain has low production cost; the method for producing the feeding proteins by using the bare-glass fly arthrobacter strain to ferment and decompose the gossypol in the cottonseed cake dregs has simple process; and after the cottonseed cake dregs are fermented and detoxicated, free gossypol content is reduced by 60 percent, and nutritive values of cotton dreg substrates are improved.

Description

technical field [0001] The invention relates to an original Arthrobacter glassifina strain, in particular to a method for using it to ferment cottonseed cake to degrade gossypol to produce feed protein, and belongs to the field of biotechnology. Background technique [0002] my country is a major cotton-producing country in the world, and its cotton output ranks first in the world. The annual output of cottonseed cake is about 4-5 million tons, accounting for 30% of the total output of various plant cakes. Cottonseed cake is not only rich in resources and widely distributed, but also has high nutritional value. The protein content is about 40%, and the amino acid composition is also good. However, because cottonseed kernels and cottonseed cake contain toxic substances such as gossypol, its use is greatly restricted Over the years, about 80% of my country's cottonseed cake resources have been used as fertilizer to return to the field, and less than 30% have been used as feed p...

AI Technical Summary

Problems solved by technology

Cottonseed cake is not only rich in resources and widely distributed, but also has high nutritional value. The protein content is about 40%, and the amino acid composition is also good. However, because cottonseed kernels and cottonseed cake contain toxic substances such as gossypol, its use is greatly restricted Over the years, about 80% of my country's cottonseed cake resources have been used as fertilizer and returned to the field, and less than 30% of the protein resources used as feed have caused a great waste of protein

At present, physical and chemical methods are commonly used to detoxify cottonseed cake. Although the content of free gossypol can be reduced after treatment, the nutritional content of the processed product is severely damaged, and the palatability and feed intake of animals are also reduced.