764 results about "Trichoderma viride" patented technology

The invention discloses a special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing one or a plurality of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaerieus, clostridium sporogenes, clostridium acetobutylicum, clostridium barati, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichodermareesei and aspergillus niger. Simultaneously, by taking the special microorganism composite bacterial agent as a vaccination bacterial agent, the invention provides an application method for decomposing the crops straws and accelerating the ferment to generate marsh gas; and the microorganism composite bacterial agent-treated crops straws have fast ferment speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no faeces of poultry, and have very wide application range.

The invention discloses a method for producing an organic fertilizer by composting and quickly fermenting traditional Chinese medicine dregs. The method comprises the following steps of (1) uniformly mixing more than two optional dregs, and controlling water content to be 50 percent to 70 percent at normal temperature; and (2) matching compound bacteria, namely mixing four microbes, namely bacillus subtilis, aspergillus niger, trichoderma viride and beer yeast in a mass proportion of 3:2:3:2. The method has the advantages that firstly, the various traditional Chinese medicine dregs are mixed and composted, and any other auxiliary material, such as poultry excrements or crop straws, do not need to be added; secondly, the four microbes, namely the bacillus subtilis, the beer yeast, the aspergillus niger and the trichoderma viride are mixed in a mass proportion of 3:2:3:2, the activity is high, a plurality of dregs, which are mixed and composted, are fermented to decompose organic materials in the dregs so as to produce the organic fertilizer, and the microbes are small in using quantity and account for 0.2 percent of bacterial manure; thirdly, the traditional Chinese medicine dregs are fermented at a high temperature of 50 to 65 DEG C generated by the four microbes naturally, an additional heat source is not needed, the traditional Chinese medicine dregs are quickly and thoroughly decomposed in a period of 5 to 7 days, and the standard of the organic fertilizer is met.

The present invention relates to a fertilizer comprising at least one microbe selected from the group consisting of Trichoderma viride (NRRL B-50520), Scopulariopsis brevicaulis (NRRL B-50521), Scopulariopsis brevicaulis (NRRL B-50522), and combinations thereof, and a substrate which is acted upon by the at least one microbe to release nitrogen. The invention also relates to a fertilizer comprising at least one microbe selected from a keratin degrading microorganism and a keratin substrate which is acted upon by the at least one microbe to release nitrogen. The invention further relates to a method for enhancing growth of plants with the fertilizer, and methods of making a fertilizer. The invention further relates to an isolated Trichoderma viride strain (NRRL B-50520) and two isolated Scopulariopsis brevicaulis strains (NRRL B-50521, NRRL B-50522).

The invention discloses a biological modified straw adsorbent, and a preparation method and application thereof. The method comprises the steps of: crushing the straw to prepare a straw powder; mixing the straw powder with a synergistic agent and water, and inoculating microbial strain for modification to conduct fermentation; and after the fermentation, taking out the modified straw powder and drying to prepare the adsorbent. The microbial strain is Trichoderma viride CGMCC3.5455, Trichoderma koningii CGMCC3.2774, Aspergillus niger CGMCC3.4309, Rhizopus stolonifer CICC40317, and Trichoderma koningii CICC 40108. The invention uses straw powder as a carbon source to conduct solid fermentation, so as to produce the adsorbent. The production process does not produce wastewater, waste gas or waste residue, and is energy-saving and environment-friendly. The prepared adsorbent has strong capacity, and can be used for treatment of tanning wastewater and dyeing wastewater.

The invention relates to a method for producing a biologic feed from cassava lees, which comprises the following steps: preparing a lees culture medium; adding a mixture of a Trichoderma koningii suspension, a Trichoderma viride cladosporium suspension, a lactic acid bacterium seed liquid and a microzyme seed liquid to the lees culture medium for anaerobic culture and fermentation; and finally drying to obtain the biologic feed. The method has the advantages of low energy consumption, low cost, short production cycle and high production efficiency, realizes the recycling of resources and reduces the emission of pollutants. Besides, the invention shows that cassava lees can be used as the material for manufacturing a fermented feed and can improve the nutrition of the base material, a biologic feed which is rich in yeast, lactic acid bacteria and organic acid can be obtained, and the biologic feed can be used as the coarse feed for breeding cows and the like on an animal farm.

The invention discloses a strain combined formula for producing grain stillage biological ferment feed through fermenting grain stillage and a screening method of the combined strain formula. The strain combined formula comprises a grain stillage culture medium and strains; the formula of the grain stillage culture medium comprises 60 percent of fresh grain stillage, 10 percent of maize flour, 10 percent of rapeseed meal and 20 percent of water; 15mg of ferrous sulfate, 12mg of zinc sulfate, 10mg of manganese sulfate, 15mg of copper sulfate and 2g of calcium hydrogen phosphate are additionally added into each 100g of the grain stillage culture medium; the strains comprise Bacillus subtilis, Trichoderma viride, Lactobacillus acidophilus and Brettanomyces custersianus, the inoculum concentration is 1.5 percent for each strain; and the viable count of Bacillus subtilis is 1.33*10<11>cfu / mL, the viable count of Trichoderma viride is 7.0*10<10>cfu / mL, the viable count of Brettanomyces custersianus is 3.5*10<11>cfu / mL, and the viable count of Lactobacillus acidophilus is 2.1*10<10>cfu / mL.

The invention relates to a preparation method of a natural high-fiber odorless cattle and sheep feed. The prepared mixed probiotic dry powder and chopped straw feed are mixed and fermented for more than 10 days to obtain the product; the straw feed is bagasse or sugarcane leaves, cornstalks or corncobs, green pasture and mulberry stems; and the fermentation probiotics comprises saccharomyces cerevisiae, EM (Effective microorganisms) and bacillus subtilis. The preparation method comprises the steps of: mixing and crushing the following components of the feed in parts by weight: 20-30 parts of soybean meal, 10-20 parts of peanut bran, 5-10 parts of passion fruit leaf, 15-20 parts of mulberry stem, 1-3 parts of momordica grosvenori and 10-20 parts of corn; mixing with 10-25 parts by weight of running water and stirring for 15min; pouring the prepared mixed probiotic raw powder and trichoderma viride in a ratio of 1kg / t; barreling and sealing; fermenting naturally for 3-7 days; and when the fermented product emits sweet fragrance, pouring the fermented product into a stirrer, and adding 20-40 parts by weight of rice bran and stirring uniformly to obtain the fermented probiotic dry powder.

The invention discloses an enzyme-bacterium duplex straw decomposing agent and its preparation and application methods. The decomposing agent is made by mixing a composite microbial agent and a composite enzyme agent in a weight ratio of 3-4:1. The composite microbial agent includes Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Brevibacillus laterosporus and a fused Saccharomyces cerevisiae, having a weight ratio of (1-2):(1-2):(1-2):(2-3):(2-3). The composite enzyme agent includes cellulase produced by the fermentation of Trichoderma reesei, hemicellulase produced by the fermentation of Bacillus subtilis, and ligninase produced by the fermentation of Pleurotus ostreatus, having a weight ratio of (1-2):(1-2):(1-2). The decomposing agent has a double straw degrading function, allowing more efficient and more thorough biological degradation, increasing the straw degrading speed to a higher extent and shortening degrading time, and can fully decompose crop straw to fields within about a week, without affecting the planting and transplanting of next-stubble crops.

The invention provides a method for preparing a novel ruminant feed by using beneficial bacteria and plant fiber. The method comprises the following steps of: mixing various fermentation substrate raw materials in a blending mixer, sterilizing, cooling, and inoculating first mixed bacterium liquid for constant-temperature fermentation for 32-48h; and then inoculating second mixed bacterium liquid for secondary fermentation for 48-72h according to the same operation steps; carrying out the low-temperature drying on the fermented materials, and smashing to obtain a finished product, wherein the fermentation substrate comprises 8-11 parts by weight of rice husk bran, 8-11 parts by weight of sunflower husk power, 8-11 parts by weight of corn peel, 8-11 parts by weight of bran, 8-11 parts by weight of distilled spirit vinasse, 8-11 parts by weight of potato pulp, 2-5 parts by weight of apple pomace, 8-11 parts by weight of tomato pomace, 8-11 parts by weight of corn stalk powder, 8-11 parts by weight of corn glycoprotein, 2-5 parts by weight of bean dregs and 1-3 parts by weight of urea, the first mixed bacterium liquid is a mixture of trichoderma koningii and green trichoderma liquid, and the second mixed bacterium liquid is a mixture of feed bacillus and candida utilis bacteria liquid.