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Microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as preparation method and application of microbial agent

A technology of microbial inoculum and polycyclic aromatic hydrocarbons, which is applied in the field of biological treatment of environmental pollutants, and achieves the effects of easy storage and transportation, restoration of polycyclic aromatic hydrocarbon-contaminated soil, and long storage time.

Active Publication Date: 2015-01-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when these free microorganisms are added to the remediation site, they may not be well applied to the remediation of in-situ contaminated soil due to the vicious competition of the indigenous bacteria or the difficulty in adapting to the environment. The high-efficiency degradation of aromatic hydrocarbons has become one of the hot issues in the research of pollution control and remediation.

Method used

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  • Microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as preparation method and application of microbial agent
  • Microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as preparation method and application of microbial agent
  • Microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as preparation method and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The culture method of Mycobacterium flavinum CP13 bacterial strain of the present invention:

[0035] The CP13 strains were streak-inoculated on the nutrient gravy medium, cultured at 28-30°C for 48h, and then respectively inserted into 500mL Erlenmeyer flasks, and cultured at 30°C and 150r / min for 12h. Then it was inserted into a 5L seed tank according to the inoculum amount of 5%, and cultivated for 24h at 180r / min, pH7.5, and ventilation rate 5L / min.

[0036] Nutrient gravy medium, ingredients and dosage (g / L): beef extract 3, peptone 10, NaC15, agar 20, adjust pH 7.0-7.2.

[0037] The cultivation method of Trichoderma viride of the present invention: inoculate the strain (Trichoderma viride GIM3.141, purchased from Guangdong Microbial Culture Collection Center) into PDA medium, and culture at 30° C. for 5 days.

[0038] PDA medium composition and dosage (g / L): potato 200, glucose 20, agar 20, adjust pH 7.0-7.2.

[0039] Preparation of composite carrier:

[0040] ...

Embodiment 2

[0044] After the bacteria agent prepared in Example 1 was stored at room temperature for a certain period of time, the bacteria agent was inoculated into the MSM culture solution with a pyrene concentration of 10 mg / L in a ratio of 10% (V / V), so as not to contain the bacteria agent The pyrene-containing MSM culture medium was used as a blank, placed in a shaker at 30°C, shaken and cultured at 150r / min in the dark for 2 days, and then the remaining amount of pyrene in the culture medium was determined. Depend on figure 1 It can be seen that the degradation rate of each initial concentration of pyrene maintained at 60%-66% within 170 days of storage at room temperature.

[0045] This example shows that the bacterial agent can be effectively preserved at room temperature, and the effective preservation time is more than 170 days, which provides a guarantee for the bioremediation of the environment polluted by polycyclic aromatic hydrocarbons.

Embodiment 3

[0047] The bacterium agent that embodiment 1 is made is inoculated in the MSM culture fluid of pyrene concentration 10mg / L by the ratio of 10% (V / V), is blank with the pyrene-containing MSM culture fluid that does not contain this bacterium agent, places 30 In a shaker at ℃, under the condition of 150r / min, shaking and culturing in the dark for 2 days, the remaining amount of pyrene in the culture solution was measured. Depend on figure 2 It can be seen that the bacterial agent of the present invention can effectively degrade polycyclic aromatic hydrocarbon pyrene under the condition of pH 4-9, and has a wider pH adaptation range than free bacteria.

[0048] This example shows that the polycyclic aromatic hydrocarbon degrading microbial agent of the present invention can degrade pyrene well in a wide pH range of acidic, neutral and alkaline, which provides a guarantee for its application in different pH environments.

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Abstract

The invention relates to a microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as a preparation method and an application of the microbial agent. The microbial agent comprises the following effective components: mycobacterium gilvum CP13, trichoderma viride and rhamnolipid. The preparation method comprises the following steps: mixing bacterial solutions obtained by respectively culturing mycobacterium gilvum CP13 and trichoderma viride, simultaneously adding a composite carrier of diatomaceous earth and modified peanut shells and rhamnolipid and carrying out microbial immobilization to prepare the microbial agent. The microbial agent has super strong polycyclic aromatic hydrocarbons removal capability and meanwhile has the advantages of low cost and long shelf life; and by virtue of the microbial agent, over 90% of polycyclic aromatic hydrocarbons, such as high-concentration (50mg / kg) pyrene contained in the vegetable garden soil can be removed under conditions of room temperature (20 + / -5 DEG C) and pH of 4-9 within 36 days.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to the preparation of a high-efficiency degradation bacterial agent for polycyclic aromatic hydrocarbons and its application in wastewater biological treatment and contaminated soil restoration. Background technique [0002] Petroleum is a complex mixture containing a variety of hydrocarbons (normal alkanes, branched alkanes, aromatics, alicyclic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, naphthenic acids, etc.). With the increase of oil extraction and use, a large amount of oil and its processed products enter the environment, which inevitably pollutes the environment in the process of its extraction, transportation, refining and use. Petroleum and petroleum products generally contain toxic substances such as polycyclic aromatic hydrocarbons (PAHs) and phenols. PAHs are a type of organic poll...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N11/14C12N11/02C02F3/34B09C1/10C12R1/32C12R1/885C02F101/32
CPCB09C1/10B09C1/105C02F3/34C02F3/347C02F2101/327C12N1/14C12N1/20C12N11/02C12N11/14
Inventor 党志邓辅财郭楚玲杨琛伍凤姬廖长君卢桂宁易筱筠
Owner SOUTH CHINA UNIV OF TECH
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