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Bacillus amyloliquefaciens and microbial inoculum and application thereof

A technology for dissolving amyloid spores and bacilli, applied in the application, fungicides, bacteria and other directions, can solve the problem that biocontrol bacteria and pathogenic bacteria do not have selective antagonism, etc., and achieve the effect of a wide range of sterilization applications.

Active Publication Date: 2015-12-09
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a strain of Bacillus amyloliquefaciens, which solves the problem in the prior art that Bacillus amyloliquefaciens has no selective antagonism to biocontrol bacteria and pathogenic bacteria

Method used

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  • Bacillus amyloliquefaciens and microbial inoculum and application thereof
  • Bacillus amyloliquefaciens and microbial inoculum and application thereof
  • Bacillus amyloliquefaciens and microbial inoculum and application thereof

Examples

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Embodiment 1

[0043] Example 1: Isolation and identification of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) HC200

[0044] 1. Isolation of Bacillus amyloliquefaciens HC200

[0045] Soil samples were collected from the mangrove silt soil in Wenchang, Hainan Province, China, air-dried the soil samples, weighed an appropriate amount of soil samples after sieving, put them into a triangular flask filled with sterile water and glass beads, and fully oscillated for 30 minutes until the soil samples were uniform After dispersing and standing for 10 minutes, dilute to different concentrations according to the gradient method. Each gradient dilution solution is heated in a water bath at 80°C for 20 minutes, and then take a certain amount of dilution solution and spread it on the chitosan selective medium. After culturing for 2-4 days, pick and purify a single colony as the strain to be tested.

[0046]Preliminary screening of Bacillus amyloliquefaciens: prepare about 1.6×10 5 Each / mL o...

Embodiment 2

[0057] Embodiment two: the method for preparing bactericide by Bacillus amyloliquefaciens HC200CGMCCNo.10371:

[0058] Bacillus amyloliquefaciens HC200CGMCCNo.10371 The method for preparing fungicides: take the Bacillus amyloliquefaciens HC200 preserved in the NA medium on the slope, streak it onto the LB medium plate, place it at 37°C, and cultivate it for 8 hours. After a single colony grows, use Pick HC200 from a sterile toothpick and inoculate it into a Erlenmeyer flask (50mL) containing 20mL of LB culture medium, place it on a shaker, and culture it at 37°C at a constant temperature of 160rpm / min for 2-3h; The volume percentage content is 1% the inoculum amount is inserted in the Erlenmeyer flask (500mL) that 200mLLB culture solution is housed; Under the condition of 37 ℃ after inoculation, shake culture with the rotating speed of 160rpm / min for 14h, obtain the amyloliquefaciens spore Bacillus HC200 fermentation broth, namely fungicide. The bactericide of this embodiment...

Embodiment 3

[0059] Embodiment three: the bactericide that embodiment two prepares is to the inhibitory action of banana fusarium wilt (Fusariumoxysporum f.sp.cubense) mycelia

[0060] 1. Spread the spore suspension of Fusarium wilt on the PDA medium plate. After pre-cultivating at 28°C for 1 day, punch a hole with a 6mm hole punch at the center of the plate and 2cm away from the center, and add 20 μL of the fungicide prepared in Example 2 In the control experiment, clean water and the pesticide carbendazim were added instead, and the inhibition zone was observed. They were cultured in an incubator at 28°C for 3 days, and each experiment was repeated 3 times. Observing its antibacterial zone, the average width of the experimental antibacterial zone is 17mm, which is equivalent to the chemical pesticide carbendazim. Figure 2-A .

[0061] 2. After melting the PDA culture medium, add the bactericide prepared in Example 2 to mix, draw an appropriate amount of mixed solution and place it on a...

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Abstract

The invention discloses bacillus amyloliquefaciens and the microbial inoculum and application thereof. Bacillus amyloliquefaciens is a Latin name, the strain number is HC200, and the accession number in the China General Microbiological Culture Collection Center is CGMCC No.10371. The bacillus amyloliquefaciens has a selective antagonism effect on biocontrol bacteria and pathogenic bacteria and has a strong inhibiting effect on fusarium oxysporum, fusarium solani, phytophthora nicotianae and ralstonia solanacearum, and has a good synergetic antagonism effect on pathogenic bacteria by being matched with trichoderma viride H06.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a strain of bacillus amyloliquefaciens and its agent and application. Background technique [0002] Fusarium wilt of banana, melon wilt, tomato wilt, vanilla Plant diseases such as orchid wilt, pepper root rot, and tomato bacterial wilt are a class of devastating soil-borne diseases. At present, there is no effective chemical pesticide for the prevention and treatment of this type of disease, and the long-term use of chemical pesticides is harmful to the environment and human beings. serious threat to health. Biological control is a safe and environmentally friendly control technology, which has become a research hotspot. Compared with chemical control methods, the use of antagonistic bacteria to control plant diseases has the advantages of environmental friendliness, low cost, and pathogenic bacteria are not easy to develop drug resistance. It is the directi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01N63/04A01P3/00C12R1/07
Inventor 黄俊生汪军曹智淳梁昌聪刘磊王国芬杨腊英郭立佳
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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