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Method for producing microbial feed additive by utilizing potato vinasse

A microbial feed and additive technology, applied in animal feed, animal feed, applications, etc., can solve the problems of high crude fiber content of distiller's grains, limit the use of distiller's grains, and high operating costs, so as to achieve a wide range of applications, improve feed conversion efficiency, The effect of high production efficiency

Inactive Publication Date: 2010-12-01
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Potato distiller’s grains are different from corn grains and rice grains in that they have deep color, high viscosity, and many fibers, and it is difficult to separate solids and liquids in the early stage. Therefore, the preparation of DDGS from potato distillers grains by means of concentration and drying will cost a lot due to equipment investment and drying concentration. The operating costs are so high that they are not economically viable
The third is to inoculate microorganisms to prepare fermented feed. Previous studies generally inoculate single strains such as lactic acid bacteria or yeast for fermentation to prepare lactic acid feed or single-cell protein feed. The feed obtained in this way can be stored for several months, prolonging the storage period or Improve its protein content, but the crude fiber content of distiller's grains is high, which limits the range of utilization of distiller's grains as feed, especially in non-ruminant farms.

Method used

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  • Method for producing microbial feed additive by utilizing potato vinasse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Fresh potato distiller's grains in weight ratio: bran: soybean meal is 5:3:2, fresh potato distiller's grains, bran and soybean meal are mixed, and 1.0% (NH 4 )2 SO 4 , and 0.2% KH 2 PO 4 Make distiller's grains medium, mix the prepared Bacillus subtilis suspension, Trichoderma konningen suspension, and Trichoderma viride in a volume ratio of 1:1:1 and add to the above-mentioned medium, every 100 grams Add 20ml of distiller's grain medium, and after cultivating and fermenting at 28°C for 72h, inoculate 10ml of activated Candida tropicalis per 100g of medium in the fermented medium (the number of bacteria is 1.6×10 6 tropicalis at a ratio of 1 / mL), cultured at 28°C for 120 h, and then dried. After determination, the protein content of the product increased by 42.8%, the cellulose content decreased by 8.3%, and the number of bacillus was 2.1×10 9 cells / mL, the number of yeasts was 1.7×10 7 individual / mL.

Embodiment 2

[0035] Fresh cassava distiller's grains in weight ratio: bran: soybean meal is 5:3:2, fresh cassava distiller's grains, bran and soybean meal are mixed, and 1.5% (NH 4 ) 2 SO 4 , and 0.2% KH 2 PO 4 Make distiller's grains medium, mix the prepared Bacillus subtilis suspension, Trichoderma konningen suspension, and Trichoderma viride in a volume ratio of 1:1:1 and add to the above-mentioned medium, every 100 grams Add 20ml of distiller's grain medium, and after cultivating and fermenting at 29°C for 48h, inoculate 10ml of activated Candida tropicalis per 100g of medium in the fermented medium (the number of bacteria is 3.0×10 6 tropicalis at a ratio of 1 / mL), cultured at 28°C for 120 h, and then dried. It was determined that the protein content of the product increased by 37.8%, the cellulose content decreased by 7.6%, and the number of bacillus was 1.9×10 8 cells / mL, the number of yeasts was 2.7×10 7 individual / mL.

Embodiment 3

[0037] Fresh sweet potato distiller's grains by weight: bran: soybean meal is 5:3:2, fresh sweet potato distiller's grains, bran and soybean meal are mixed, and add 1.2% (NH 4 ) 2 SO 4 , and 0.2% KH 2 PO 4 Make the distiller's grains medium, mix the prepared Bacillus subtilis bran koji, Trichoderma koningensis bran koji, and Trichoderma viride koji in the weight ratio of 1:1:1 and add to the above-mentioned culture medium, every 100 grams of distiller's grains Add 20 grams to the culture medium, cultivate and ferment for 24 hours at 28 ° C, then inoculate 10 ml of activated Candida utilis (the number of bacteria is 1.0 × 10 6 Candida utilis was inoculated at a ratio of 1 / mL), cultured at 28°C for 120 h, and then dried. It was determined that the protein content of the product increased by 38.4%, the cellulose content decreased by 7.9%, and the number of bacillus was 2.0×10 9 cells / mL, the number of yeasts is 1.2×10 7 individual / mL.

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Abstract

The invention relates to a method for producing a microbial feed additive by using potato vinasse. The method comprises the following steps of: preparing a vinasse culture medium; mixing bacillus subtilis, trichoderma koningii and bacterial suspension liquid of trichoderma viride spores or mouldy bran, and adding into the vinasse culture medium for culturing and fermenting; and inoculating candida tropicalis or candida utilis seed liquid in the well-fermented culture medium for continuously culturing and fermenting; and finally drying to obtain the microbial feed additive. The invention has the advantages of low energy consumption, low cost, short production period, high production efficiency and recycled resources, reduces the emission of pollutants and is beneficial to building an environment-friendly and resource-saving society; and the produced additive has wide application range, can be added into animal feeds and is a high-quality and highly-efficient microbial fermentation product.

Description

technical field [0001] The invention relates to a production method of a microbial feed additive, in particular to a method for producing a microbial feed additive by using the by-product potato distiller's grains after brewing alcohol in a brewery. Background technique [0002] With the increasing scale of ethanol production in the world, people are facing another problem: how to efficiently utilize alcohol waste residue and waste liquid to maximize the profit of the alcohol industry, reduce waste emissions, reduce environmental pollution, and achieve energy saving, emission reduction, The dual purpose of comprehensive utilization. Alcohol waste liquid is the main by-product of the winery, its volume is about 10 times the alcohol production. All countries in the world are trying to solve the problem of distiller's grain utilization. Potato distiller's grains mainly include potato distiller's grains, cassava distiller's grains, and sweet potato distiller's grains. The prot...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K10/38
CPCY02P60/87
Inventor 周红丽熊兴耀谭兴和苏小军王锋李清明
Owner HUNAN AGRICULTURAL UNIV
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