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Enzyme-bacterium duplex straw decomposing agent and its preparation and application methods

A technology of straw decomposing agent and compound bacterial agent, which is applied in the direction of biochemical equipment and methods, preparation of organic fertilizers, and methods based on microorganisms, and can solve the problems of using microorganisms to decompose straws, etc.

Inactive Publication Date: 2016-03-02
甘肃明德伟业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many microorganisms can decompose cellulose alone, the use of microorganisms to decompose straw is limited due to the above reasons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] An enzyme-bacteria double-linked straw decomposing agent, which is composed of a mixture of a microbial compound inoculum and a compound enzyme in a weight ratio of 4:1. The microbial compound inoculum includes Trichoderma viride, Aspergillus niger, Aspergillus oryzae, and Brevibacterium lateralis Bacillus and yeast fusion bacteria, the weight ratio of Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Brevibacterium lateralis and yeast fusion bacteria is 2:1:1:3:3, and the complex enzymes include those produced by fermentation of Trichoderma reesei Cellulase, hemicellulase produced by fermenting Bacillus subtilis, ligninase produced by fermenting Pleurotus ostreatus, the weight ratio of cellulase, hemicellulase and ligninase is 1:1:1.

[0054] The preparation method of the enzyme-bacteria double-linked straw decomposing agent of the present invention includes the following steps:

[0055] 1. Preparation of microbial compound inocula:

[0056] The microbial composite ...

Embodiment 2

[0129] An enzyme-bacteria double-linked straw decomposing agent, which is composed of a 3:1 weight ratio of microbial composite inoculants and composite enzymes. The microbial composite inoculants include Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Brevibacterium lateralis Bacillus and yeast fusion bacteria, the weight ratio of Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Brevibacterium lateralis and yeast fusion bacteria is 1:2:2:2:2, and the complex enzymes include those produced by fermentation of Trichoderma reesei Cellulase, hemicellulase produced by fermenting Bacillus subtilis, and ligninase produced by fermenting Pleurotus ostreatus, the weight ratio of cellulase, hemicellulase and ligninase is 1:2:2.

[0130] The preparation method of the enzyme-bacteria double-linked straw decomposing agent of the present invention includes the following steps:

[0131] 1. Preparation of microbial compound inocula:

[0132] The microbial composite fungus for di...

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Abstract

The invention discloses an enzyme-bacterium duplex straw decomposing agent and its preparation and application methods. The decomposing agent is made by mixing a composite microbial agent and a composite enzyme agent in a weight ratio of 3-4:1. The composite microbial agent includes Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Brevibacillus laterosporus and a fused Saccharomyces cerevisiae, having a weight ratio of (1-2):(1-2):(1-2):(2-3):(2-3). The composite enzyme agent includes cellulase produced by the fermentation of Trichoderma reesei, hemicellulase produced by the fermentation of Bacillus subtilis, and ligninase produced by the fermentation of Pleurotus ostreatus, having a weight ratio of (1-2):(1-2):(1-2). The decomposing agent has a double straw degrading function, allowing more efficient and more thorough biological degradation, increasing the straw degrading speed to a higher extent and shortening degrading time, and can fully decompose crop straw to fields within about a week, without affecting the planting and transplanting of next-stubble crops.

Description

Technical field [0001] The invention belongs to the technical field of crop stalk treatment, and specifically relates to an enzyme-bacteria dual straw decomposing agent and a preparation method and a use method thereof. Background technique [0002] my country is a large agricultural country with abundant straw resources of various crops, with an annual output of more than 700 million tons of straw. With the resolution of the rural energy problem, straw is no longer used as the main fuel, and the phenomenon of centralized burning has become increasingly prominent, which not only pollutes the environment and endangers people's health, but is also filled with smog, which affects land transportation and flight safety. The main components of straw are cellulose (about 42%), hemicellulose (about 32%) and lignin (about 8%), and contain nitrogen, phosphorus, potassium, calcium, magnesium and organic matter. Therefore, the rational development and utilization of crop straw is particular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N9/42C12N9/08C05F17/00C05F11/00C05G1/00C12R1/885C12R1/685C12R1/69C12R1/85C12R1/13
CPCC05C9/00C05F17/00C12N1/14C12N1/20C12N9/0061C12N9/0065C12N9/2437C12N9/2477C12Y110/03002C12Y111/01013C12Y111/01014C12Y302/01004C05F11/00C05F11/08Y02W30/40
Inventor 王宝民张恩辰张锦绣张兴隆黄佳
Owner 甘肃明德伟业生物科技有限公司
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