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Aspergillus niger genetic engineering strain with high yield of organic acid under low-dissolved oxygen condition and application thereof

A genetically engineered strain, Aspergillus niger technology, applied in the field of genetic engineering, can solve problems such as patent publications that have not yet been found

Active Publication Date: 2019-07-19
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY +1
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[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Aspergillus niger genetic engineering strain with high yield of organic acid under low-dissolved oxygen condition and application thereof
  • Aspergillus niger genetic engineering strain with high yield of organic acid under low-dissolved oxygen condition and application thereof
  • Aspergillus niger genetic engineering strain with high yield of organic acid under low-dissolved oxygen condition and application thereof

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Embodiment Construction

[0039] The present invention will be described in further detail below in conjunction with specific examples. The following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0040] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0041] A kind of aspergillus niger (Aspergillus niger) genetically engineered bacterial strain resistant to low dissolved oxygen condition high yield organic acid, the construction steps of described genetically engineered bacterial strain are as follows:

[0042] Step 1, construction of heterologously expressed vhb gene plasmid: First, a codon-optimized vhb gene sequence fragment was synthesized by Beijing Huada Gene Company. The nucleotide sequence of the gene vhb sequence fragment was SEQ NO.1, and the...

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Abstract

The invention relates to an Aspergillus niger genetic engineering strain with high yield of organic acid under a low-dissolved oxygen condition. The construction steps of the genetic engineering strain are as follows: step 1, constructing a heterologously-expressed vhb gene plasmid, wherein the sequence fragment of the gene vhb is controlled by an Aspergillus niger 3-glycerophosphate dehydrogenasegene promoter PgpdA; and step 2, acquiring a heterogeneously-expressed vhb gene strain so as to obtain the Aspergillus niger genetic engineering strain with high yield of organic acid under a low-dissolved oxygen condition. Based on the natural characteristics that Aspergillus niger generates organic acid, the Aspergillus niger genetic engineering strain is obtained by modifying the physiologicalcharacteristics of Aspergillus niger through genetic recombination. Experiments prove that the ability of the aspergillus niger genetic engineering strain for producing organic acid under the condition of low dissolved oxygen is obviously improved, and an excellent strain is provided for preparing organic acid by a microbial fermentation method.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a genetically engineered strain of Aspergillus niger (Aspergillus niger) resistant to low dissolved oxygen conditions and high in organic acid production and its application. Background technique [0002] As an important cell factory, Aspergillus niger has been used in the fermentative production of organic acids for more than 100 years. It is not only a GRAS (generally regarded safe) strain, but also able to utilize cheap carbon sources. Citric acid and L-malic acid are the two most important organic acids in food, medicine and other industries. In the market of all organic acids, citric acid has a market share of more than 70%. It can be used as a flavoring agent and as an antioxidant for edible oils. At the same time, it improves the sensory properties of food, enhances appetite and promotes the digestion and absorption of calcium and phosphorus substances in the ...

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Application Information

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IPC IPC(8): C12N1/15C12N15/31C12N15/80C12N15/66C12P7/46C12P7/48C12R1/685
CPCC07K14/195C12N15/80C12N15/66C12P7/46C12P7/48C12N2800/22
Inventor 刘浩周宇涛黄和徐永学徐晴曹威
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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