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Method for producing potassium gluconate through fermentation of Aspergillus niger

A fermentation technology of potassium gluconate and Aspergillus niger, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of lower glucose conversion rate, low sugar concentration at the beginning of fermentation, and increase catalyst consumption, etc., to achieve Facilitate extraction, high concentration, and reduce steam consumption

Inactive Publication Date: 2012-12-12
山东凯翔生物科技股份有限公司
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AI Technical Summary

Problems solved by technology

[0004] The first method decalcification method produces insufficient potassium gluconate:
[0005] 1. High production cost: Since the solubility of calcium gluconate is less than 4%, the initial sugar concentration of fermentation is low (<15%), and the concentration of the final reaction product calcium gluconate fermentation liquid is lower than 15%, which leads to high production cost;
[0006] 2. Cause environmental pollution: add sulfuric acid to produce solid waste calcium sulfate at the same time, which is difficult to handle and cause pollution;
[0007] 3. Long process line, large investment and high energy consumption: first extract calcium gluconate, then dissolve crystals and add KOH solution for reaction, and then extract potassium gluconate, which requires two evaporations, crystallization, centrifugal separation, and drying, resulting in large investment , high energy consumption and high production cost;
[0010] 1. After the catalyst used is recycled for a certain number of times, the catalytic efficiency will decrease, the conversion rate of glucose will decrease, the reaction time will be extended, and the catalyst will have no catalytic activity. The catalyst must be scrapped and replaced, which will correspondingly increase the catalyst consumption per unit product and make the production cost of the product relatively low. high;
[0011] 2. The process line is long and the cost is high;
[0012] 3. Due to the certain toxicity of metal catalysts, its products are usually not used as food additives in food production and are not safe; therefore, the application of the products is subject to certain restrictions

Method used

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  • Method for producing potassium gluconate through fermentation of Aspergillus niger

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Embodiment Construction

[0027] 1. Preparation of glucose solution:

[0028] 1) Mixing:

[0029] According to the ratio of starch and starchy raw materials to water 1:1-2 (W / V), add starch into the slurry tank, adjust the pH value to 6.0-6.5 with sulfuric acid or KOH, and dry at 8-20u / g Add high-temperature α-amylase to the starch; stir for 20-30 minutes.

[0030] 2) Jet liquefaction:

[0031] Using a jet liquefaction process, turn on the starch pump to control the slurry for 5-50m 3 / h flow into the ejector: the feed pressure is greater than the steam pressure 0.02-0.04Mpa, the temperature is controlled at 100-115 ° C, the slurry and steam are quickly mixed into the pressure tank, maintained for 5-6 minutes, and then into the flash tank. After the material temperature drops to 95--98°C, it enters the maintenance tank and maintains for 90-120 minutes. After passing the iodine inspection, the material temperature is reduced to 62--64°C through the heat exchanger and enters the pH mixing tank.

[00...

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Abstract

The invention discloses a method for producing potassium gluconate through fermentation of Aspergillus niger, which comprises the following steps: performing one-step jet on starch and starch-containing materials, and performing double-enzyme glucose preparation; using an Aspergillus niger P1205X21 strain which is resistant to high-concentration K<+> to ferment the glucose solution, and feeding an ion membrane KOH solution in the fermentation process to obtain a potassium gluconate solution; and by using a thermal crystallization process of multiple-effect continuous evaporation and crystallization, directly evaporating and crystallizing in a multiple-effect evaporation crystallizer to obtain the potassium gluconate finished product. The invention has the advantages that the raw materials are rich and accessible, the cost is low, the fermentation product is simplex, the product quality is high and easy to control, no waste water and waste gas are generated, no calcium gluconate mother liquor and calcium sulfate waste are generated, the environmental pollution is greatly reduced, the process route is short, evaporation, crystallization, drying and other procedures during calcium gluconate preparation are reduced, vapor is saved, the power consumption is reduced, and the production cost is greatly lowered.

Description

technical field [0001] The invention relates to a method for fermenting and producing potassium gluconate, in particular to a method for producing potassium gluconate through double-enzyme sugar production and submerged aerobic fermentation by using Aspergillus niger strains to utilize starch and starchy raw materials. Background technique [0002] Potassium gluconate is used as a nutritional supplement, acidity regulator, chelating agent, yeast food, etc. It is mostly used in solid beverages, cake premix powder, dessert premix powder, etc. Mainly used as nutrition, dietary additive, sequestering agent. It is an effective potassium supplement. It can maintain intracellular osmotic force, regulate the acid-base balance of the human body, and play an important role in maintaining skeletal muscle tension. It is an effective potassium supplement for various potassium deficiency diseases in the human body and a good food fortifier for potassium. There are many types of potassiu...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12R1/685
Inventor 李贵伶崔光水徐月美张伟
Owner 山东凯翔生物科技股份有限公司
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