Method for promoting chlorella growth

A technology of chlorella and algae liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, adding compounds to stimulate growth, etc., can solve the problems of out-of-sync growth rate and inability of the reaction system to exert the best effect, etc. Achieve the effect of good ecological security and strong algae-promoting activity

Inactive Publication Date: 2015-11-18
SHANDONG UNIV OF TECH
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  • Abstract
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Problems solved by technology

However, its rapid growth rate is out of sync with the requirements of the bioreactor, resulting in the inability of the reaction system to perform optimally

Method used

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  • Method for promoting chlorella growth

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Experimental program
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Effect test

Embodiment 1

[0020] High-density cultivation of algae to promote experiments:

[0021] The experiment was carried out in a 250mL Erlenmeyer flask, and the long-term vigorous Chlorella was inoculated into 100mL culture medium, so that the initial absorbance of the algae liquid reached 0.25. Set up an experimental group and a control group (no algae promoting agent), and add pectinase and (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole to the experimental group, in which the fruit The dosage of gluease is 800mg / L, the dosage of (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole is 0mg / L, and the dosage frequency is once, and then every day Count the growth of algae. After adding pectinase and (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole, the growth of Chlorella was promoted after 9 days of culture, and the growth promotion rate reached 105.7% ( figure 1 ).

Embodiment 2

[0023] High-density cultivation of algae to promote experiments:

[0024] The experiment was carried out in a 250mL Erlenmeyer flask, and the long-term vigorous Chlorella was inoculated into 100mL culture medium, so that the initial absorbance of the algae liquid reached 0.25. Set up an experimental group and a control group (without algae promoting agent), three in parallel, and add pectinase and (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyridine to the experimental group azole, wherein the dosage of pectinase is 800mg / L, the dosage of (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole is 20mg / L, and the dosage frequency is Once, then count the growth of algae every day. After adding pectinase and (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole, the growth of Chlorella was promoted after 9 days of culture, and the growth promotion rate reached 35.7% ( figure 1 ).

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Abstract

The invention discloses a method for promoting chlorella growth. The invention discloses application of pectinase and (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole in promoting algae growth. The pectinase is a common enzyme in the food field, and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole is a substance capable of promoting pectinase activity, which can avoid influence on other organisms in environment. A using method has the characteristics that the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole is added to a water body when the chlorella grows to a certain stage, so that a promoting effect is taken when the chlorella just begins to grow to keep algae at a relatively high density and to effectively promote algae growth; the pectinase and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole have the features of being safe, efficient, degradable and the like; and the pectinase and the (2,4,6-trihydroxylphenyl)-4-(4-hydroxyphenyl) pyrazole can be extensively applied to a natural or landscape water body.

Description

technical field [0001] The invention belongs to the fields of biology and environment, and particularly relates to a method for promoting the growth of chlorella. The invention belongs to the technical field of ecological algae promotion, and relates to a pectinase and a compound (2,4,6-trihydroxyphenyl)-4-(4-hydroxyphenyl)pyrazole which can promote the activity of pectinase. Application in promoting the growth of algae, using their synergistic effect to promote the growth of algae. Background technique [0002] Chlorella is a eukaryotic single-celled algae and the first artificially cultured microalgae. It has extremely rich and balanced nutrients and excellent health care effects, and is widely used in sewage treatment, health food, and aquaculture. In the production of bait and livestock feed additives, it is known as medicinal algae. [0003] In recent years, with the rapid rise of algae biotechnology, Chlorella has been used as a bioreactor to produce drugs because of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
Inventor 冯传兴周泉城刘娜张光耀杨晓玉
Owner SHANDONG UNIV OF TECH
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