Pectinase preparation producing Aspergillus japonicus PJ01 and enzyme production method

Abstract

The invention relates to a bacterial strain for fermentation of orange peel powder to produce a pectinase preparation and a method of enhancing enzyme activity by a surfactant. In the invention, Aspergillus japonicus PJ01 is employed to produce the pectinase preparation, the activity of the prepared pectinase preparation is represented by pectinase activity, cellulase activity and hemicellulase activity. The production method of the pectinase preparation includes: taking crushed orange peel powder as a sole carbon source, adding the orange peel powder into an inorganic nutrient salt in a Czapek medium, then adding 0.1-0.5%(wt) of PEG4000 into a liquid Aspergillus niger fermentation medium, carrying out shaking cultivation for 3-5 days, thus significantly improving the exo-pectinase activity, the cellulase activity (in terms of CMCase enzyme) and hemicellulase activity (in terms of Xylanase) relative to the control group. This method is simple, can significantly improve the pectinase preparation production ability of Aspergillus, and has the characteristic of environmental friendliness, thereby having good application prospects.

Description

technical field [0001] The invention relates to a bacterial strain producing pectinase preparation and an enzyme production method for improving its enzyme activity, in particular to a bacterial strain for producing pectinase preparation by fermenting orange peel powder and an enzyme production method for improving its enzyme activity by using a surfactant. Background technique [0002] During the processing of citrus, a large amount of juice, essential oil and other by-products will be produced, among which orange peel is a major solid by-product, accounting for about 50% of the fresh weight of the fruit. At the same time, fresh orange peel is used as Disposal of waste puts a lot of pressure on the environment. Taking China as an example, the country produces more than 5 million tons of peels every year, and the output of citrus peels in Hunan Province alone is as high as 650,000 tons. Therefore, how to use these "garbage" to develop a series of high value-added products i...

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Problems solved by technology

[0006] It is well known that fungi such as Aspergillus, Trichoderma and other fungi produce extracellular enzymes when degrading plant cell walls, so the secretion of extracellular enzymes is directly related to cell permeability. Tangnu et al. found that an appropriate concentration of Tween-80 can promote Trichoderma to secrete cellulose The ability of enzyme, hemicellulase and β-glucosidase, Zeng Guangming and others significantly improved the enzyme activity by adding rhamnolipid in the medium of Trichoderma viride fermented cellulose, Callow et al. found that rhamnolipid and Triton X-100 can promote the ability of Trichoderma reesei to produce cellulase, but the use of surfactants to promote the degradation of orange peel powder to produce enzymes is a blank

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