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Method for measuring activity of ruminant tumor gastric juice cellulase

A cellulase, ruminant technology, used in the determination/inspection of microorganisms, biochemical equipment and methods, etc.

Inactive Publication Date: 2008-07-30
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

[0003] At present, the determination of cellulase activity is only found in the determination of enzyme activity in papermaking and feed industries. There is no recognized method for the determination of rumen cellulase activity in China. The determination of cellulase activity in rumen fluid can directly measure the fibrosis in the rumen. The degree of degradation of feed substances indirectly reflects the changes in the number of microorganisms and related flora producing cellulase in the rumen, and provides a reliable basis for elucidating the enzymatic mechanism of fiber degradation in the rumen, so it has important practical value

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  • Method for measuring activity of ruminant tumor gastric juice cellulase

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Embodiment Construction

[0006] The present invention is further described by taking various typical enzymes of the above three types of cellulase.

[0007] 1. Determination method and steps of rumen fluid Avicelase activity:

[0008] A. Solution preparation:

[0009] (1) 0.2mol / L phosphate buffer solution: Dissolve 27.36g of sodium dihydrogenphosphate (NaH2PO4·2H2O) and 6.61g of disodium hydrogenphosphate (Na2HPO4) in 100mL of water, and adjust the pH to 6.0.

[0010] (2) 0.5% ball-milled filter paper substrate solution: Cut the filter paper (Whatwan No.1) into thin strips, weigh 0.5g into a Erlenmeyer flask, add 100mL 0.2mol. / L phosphate buffer solution and an appropriate amount of glass beads, and cover it Shake the bottle mouth at 65°C for 12 hours on a water bath shaker or at room temperature for 72 hours until it becomes a uniform paddle.

[0011] (3) 0.5% salicin (silicin) substrate solution: 0.5 g of salicin was dissolved in 100 mL of 0.2 mol / L phosphate buffer solution.

[0012] (4) 0.5% s...

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Abstract

The invention belongs to the technical field of animal husbandry, and is a method of detecting the activity of cellulose in tumor gastric juice of ruminant. Testing subjects can be three fistula cows, and tumor gastric juice can be sampled 2h after the early feeding to be used for conducting the external detection of the activity of cellulose. Cellulose mainly contains: (1) microcrystalline cellulose, (2) carboxymethyl cellulose and (3) salicin.

Description

technical field [0001] The invention relates to a method for measuring cellulase activity, more specifically, the invention relates to a method for measuring cellulase activity in rumen fluid of ruminants. Background technique [0002] The existing technology shows that before the cell wall material is decomposed by the cellulolytic bacteria, the fiber material should first be oxidized and the physical and chemical structure change. , and only when the adhesion ability of cellulolytic bacteria to fibers is large enough, accompanied by the production of sufficient enzymes, can the real fiber decomposition occur. The digestion of carbohydrates in the rumen is actually a continuous cycle process in which microorganisms consume soluble carbohydrates and continuously produce cellulolytic enzymes to degrade crude fiber. At present, cellulase is known as an enzyme system, and there are mainly three types: the first category includes: exo-cellulase, or exo-Bβ-1,4-glucanase, microcr...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
Inventor 刘春龙李忠秋孙海霞李长胜
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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