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New fungus Acremonium sp. DPZ-SYz-2-3 for high efficiency cellulose degradation and application thereof

A technology for degrading bacteria and cellulase, which is applied in the biological field and can solve the problems of low efficiency and industrial utilization

Inactive Publication Date: 2012-02-29
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the low efficiency of degrading lignocellulose into monosaccharides is a factor restricting its industrial utilization.

Method used

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  • New fungus Acremonium sp. DPZ-SYz-2-3 for high efficiency cellulose degradation and application thereof
  • New fungus Acremonium sp. DPZ-SYz-2-3 for high efficiency cellulose degradation and application thereof
  • New fungus Acremonium sp. DPZ-SYz-2-3 for high efficiency cellulose degradation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1. Materials and methods

[0022] 1. Materials

[0023] 11 Soil sample collection

[0024] The samples were collected from the rhizosphere deposits of Rhizophora stylosa in the mangrove area along the Hongsha River in Sanya City, Hainan Province in September 2010. After the samples were collected, they were put into sterilized sealed polyethylene bags and brought back to the laboratory at a low temperature of 4°C. save.

[0025] 1.2 Medium

[0026] 1.2.1 Separation medium (g / L):

[0027] The preparation method of every liter of separation medium is as follows: Potato juice (peel the potato, dig out the bud eyes, wash, slice. Weigh 200g and put it into 1000ml tap water, boil with a slow fire for 30min, filter with double gauze, add water to the filtrate to make up to 1000ml) 800ml , sucrose, 20.0g; NaCl, 1.5g; agar, 15g, soil extract, 200ml; after sterilizing at 121°C, wait for the medium to cool to 60°C, add filter-sterilized 100μg / ml ampicillin and 100μg / ml 1ml ea...

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Abstract

The invention discloses a new fungus Acremonium sp. DPZ-SYz-2-3 for the high efficiency cellulose degradation and an application thereof. Acremonium sp. DPZ-SYz-2-3 is preserved in the China general microbiological culture collection center (CGMCC) on 05 / 16 / 2011 and has a preservation number of CGMCC No: 4810, wherein the address of the CGMCC is No.3 of No.1 Beichen Road West, Chaoyang District, Beijing, Institute of Microbiology Chinese Academy of Sciences. The fungus of the invention, which has a cellulase production activity, can be used for producing cellulase, so the fungus has important values to the production and the utilization of cellulase; and the fungus can be further used for making a biological organic matter degradation bacterial fertilizer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new high-efficiency cellulose-degrading fungus Acremonium sp.DPZ-SYz-2-3 and its application in producing cellulase and preparing bio-organic matter-degrading bacterial fertilizer. Background technique [0002] Mangrove is a woody plant community distributed in the intertidal zone of tropical and subtropical coasts, and it is a unique forest type on the beach (Lin P et al.1999). Although it only accounts for one-thousandth of the global land area, it forms four high-productivity marine ecosystems with high productivity equivalent to the Amazon rainforest, coral reef ecosystems, upwelling areas, and seagrass bed ecosystems (Longhan et al. , 2005). There are 15,122 hectares of mangroves in my country, which are mainly distributed in Hainan, Guangdong, Guangxi and other places. Among them, Hainan Province is the region with the most species of mangroves and the best growt...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C05F11/08C12R1/645
Inventor 潘虎张偲龙丽娟董俊德张燕英凌娟陈蕾
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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