Quick screening method for laccase high yield fungus

Abstract

The quick screening method for high yield laccase fungus includes the culture of the spawn with PDA culture medium containing guaiacol and the combined culture test after the culture medium becomes iron red. It includes the following steps: designing several PB culture medium combination based on the key factors for raising enzyme generating activity and culturing spawn; culturing for 7 days while measuring the laccase activity once every day; obtaining highest laccase activity and performing variance analysis to obtain the p values of all the factors; and estimating the effects of all factors on the laccase generation based on the p values. The present invention has greatly reduced test times, can determine the laccase producing capacity under the action of different factors, and obtain high yield laccase fungus through comparing the laccase yield in optimized condition.

Description

technical field [0001] The invention relates to a fungal breeding method, in particular to a fast and efficient screening method for bacteria with high laccase production. Background technique [0002] Laccase (EC1.10.3.2) is a class of oxidoreductases whose active center contains copper atoms, mainly produced by higher plants and fungi. Because of its wide range of substrates, it has been used in paper industry, printing and dyeing industry, food industry, etc. Because laccase can act on some organic compounds with phenolic structure, it also has strong application potential in the field of environment and soil remediation. Due to the advantages of fast growth, wide source, easy cultivation and convenient extraction of laccase, fungi have become the main material for laccase production. However, the main factor restricting the practical application of laccase is the high production cost. Screening is one of the important methods to reduce production costs. [0003] At pr...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies of existing screening methods that easily lead to deviations in results, large workloads and long cycles, and screen high-yield laccase-producing bacteria from a large number of candidate strains, the present invention will provide a quick method for screening high-yielding laccase-producing fungi, which not only greatly reduces the number of tests The number of times, and the ability of the strain to produce enzymes under the action of different factors can be determined, and finally by comparing the production of laccase under the optimized culture conditions, a high-yield laccase-producing strain can be obtained.

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