Laccase generation cerrena and application thereof
A kind of tooth hairy fungus, one color technology, applied in the direction of fungi, oxidoreductase, immobilized on or in the inorganic carrier, etc., can solve the problems of low laccase level, slow growth of bacteria, low enzyme catalytic efficiency, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] Example 1. Isolation and Identification of Cerrena unicolor GSM-01CGMCC No.7713
[0048] 1. Isolation of Cerrena unicolor GSM-01CGMCC No.7713
[0049] Two pure culture strains were obtained by tissue isolation from the fruiting bodies of the green plant Ligustrum sinense between No. 2 and No. 3 office buildings of Beijing Agricultural College, and the numbers were GSM-01 and GSM-10, respectively.
[0050] 2. Identification of Cerrena unicolor GSM-01CGMCC No.7713
[0051] Using the genomic DNA of bacterial strains GSM-01 and GSM-10 as templates, ITS sequences were amplified by PCR with ITS1 (5'TCCGTAGGTGAACCTGCGG3') and ITS4 (5'TCCTCCGCTTATTGATATGC3'), and the ITS sequences amplified by the bacterial strain GSM-01 were as follows: SEQ ID No.1, the ITS sequence amplified from strain GSM-10 is shown as SEQ ID No.2. The identity of strain GSM-01 and GSM-10 was 98.26%.
[0052] The morphological characteristics of fruiting bodies of strains GSM-01 and GSM-10 are as follow...
Embodiment 2
[0055] Example 2, Study on the Enzyme Production Characteristics of Liquid Fermentation of Cerrena unicolor GSM-01CGMCC No.7713 and Cerrena unicolor GSM-10
[0056] 1. Liquid fermentation
[0057] Cerrena unicolor (Cerrena unicolor) GSM-01CGMCC No.7713 and Cerrena unicolor (Cerrena unicolor) GSM-10 were separately inoculated in PD medium, shaken at 25°C, 180rpm (rotation radius: 20mm) for 5 days, as Seed solution; 5% (v / v) inoculum amount inoculates 100ml of PD medium in a 500ml Erlenmeyer flask, shakes at 25°C and 180rpm (rotation radius: 20mm) for 6 days, centrifuges at 12,000rpm at 4°C for 15min, collects the precipitate For the mycelia, the supernatant is collected to obtain a fermentation broth, and the obtained mycelium is ground into a homogenate for later use. Obtain Cerrena unicolor GSM-01CGMCC No.7713 mycelium, Cerrena unicolor GSM-01CGMCC No.7713 fermentation broth, and Cerrena unicolor GSM-10 mycelium respectively Body and Cerrena unicolor GSM-10 fermentation bro...
Embodiment 3
[0064] Example 3. Laccase produced by liquid fermentation of Cerrena unicolor GSM-01CGMCC No.7713
[0065] This embodiment has tested five kinds of liquid fermentation media, are respectively 0mmol / L Cu 2+ Medium, 1.0mmol / L Cu 2+ Medium, 2.0mmol / L Cu 2+ Medium, 5.0mmol / L Cu 2+ Medium and 10mmol / LCu 2+ Medium. 0mmol / L Cu 2+ The medium is PD medium. 1.0mmol / L Cu 2+ The medium is to add 1.0mol / L CuSO to PD medium 4 solution to Cu 2+ The liquid obtained at a final concentration of 1.0 mmol / L. 2.0mmol / L Cu 2+ The medium is to add 1.0mol / L CuSO to PD medium 4 solution to Cu 2+ The liquid obtained at a final concentration of 2.0 mmol / L. 5.0mmol / L Cu 2+ The medium is to add 1.0mol / LCuSO to PD medium 4 solution to Cu 2+ The liquid obtained with a final concentration of 5.0 mmol / L. 10mmol / L Cu 2+ The medium is to add 1.0mol / L CuSO to PD medium 4 solution to Cu 2+ The liquid obtained with a final concentration of 10 mmol / L.
[0066] Cerrena unicolor (Cerrena unicolor...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com