Preparation method and application for bionic laccase based on dipeptide
A biomimetic lacquer and laccase technology, applied in the field of materials, can solve the problems of difficult preparation, low catalytic efficiency, poor stability, etc., and achieve the effects of non-toxic metabolic mechanism, high catalytic efficiency and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0073] Example 1. Preparation of cationic dipeptide-glutaraldehyde-copper assembly biomimetic laccase
[0074] (1) Weigh 10 mg of lyophilized powder of cationic phenylalanine dipeptide (CDP), dissolve CDP with 1,1,1,3,3,3-hexafluoroisopropanol (referred to as HFIP) as solvent, and ultrasonically oscillate 5min, so that the powder is completely dissolved, the CDP concentration is 10mM;
[0075] (2) Use a pipette gun to draw a certain amount of CDP solution and add it to a glutaraldehyde (GA) aqueous solution with a concentration of 5 mM, wherein the volume ratio of CDP solution to GA solution is 1:10, and after shaking, incubate in the dark for 20 hours. Obtain a light yellow suspension, which is the CDP-GA nanosuspension, centrifuge and wash 3 times, add water to disperse, and obtain an aqueous dispersion of nanoparticles (the concentration of the dipeptide-crosslinking agent assembly is 2 mM);
[0076] (3) Add the 20mM copper sulfate aqueous solution to the CDP-GA nanopartic...
Embodiment 2
[0088] Example 2. Preparation of cationic dipeptide-glutaraldehyde-copper assembly biomimetic laccase
[0089] (1) Weigh 10 mg of lyophilized powder of cationic phenylalanine dipeptide (CDP), dissolve CDP with 1,1,1,3,3,3-hexafluoroisopropanol (HFIP for short), and ultrasonically shake 5min, the powder is completely dissolved, and the CDP concentration is 20mM;
[0090] (2) Use a pipette gun to absorb a certain amount of CDP solvent and add it to a 5mM glutaraldehyde (GA) aqueous solution, wherein the volume ratio of the CDP solution to the GA solution is 1:20. After shaking and incubating in the dark for 30 hours, the obtained The light yellow suspension, which is the CDP-GA nanosuspension, was centrifuged and washed 3 times to obtain an aqueous dispersion of nanoparticles, and the concentration of the dispersion was 4 mM;
[0091] (3) Add the copper sulfate aqueous solution that concentration is 50mM in step (2) gained CDP-GA nanoparticle dispersion liquid, make CDP and GA ...
Embodiment 3
[0097] Example 3. Preparation of cationic dipeptide-glutaraldehyde-copper assembly biomimetic laccase (directly mix three components to prepare biomimetic laccase)
[0098] (1) Weigh 10 mg of lyophilized powder of cationic phenylalanine dipeptide (CDP), dissolve CDP with 1,1,1,3,3,3-hexafluoroisopropanol (HFIP for short), and ultrasonically shake 5min, so that the powder is completely dissolved, the CDP concentration is 10mM;
[0099] (2) Dilute the glutaraldehyde (GA) aqueous solution with an initial concentration of 0.1M, and the dilution factor is 20 times to obtain a GA aqueous solution with a concentration of 5mM;
[0100](3) Weigh 0.2 g of copper sulfate pentahydrate powder, dissolve it in deionized water, and configure it into an aqueous solution of copper sulfate with a concentration of 40 mM;
[0101] (4) Mix the solution configured in steps (1), (2), and (3) with a pipette gun, and the volume ratio of the CDP solution, GA solution and copper sulfate solution is 1:10...
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com