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Preparation method and application of bionic laccase functionalized imine covalent organic framework nano-enzyme

A technology of covalent organic framework and imines, which is applied in the field of nano-enzyme biomaterials, can solve problems such as low structural activity and unreasonable structural design, and achieve enhanced catalytic activity, excellent structural stability, and enhanced catalytic ability. Effect

Active Publication Date: 2021-06-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems of low biomimetic structure activity and unreasonable structure design of laccase nanozyme at present, the present invention proposes to construct biomimetic functional units with laccase-like active centers in the imine COFs framework (Am-COFs) to realize phenolic Efficient degradation of similar pollutants

Method used

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  • Preparation method and application of bionic laccase functionalized imine covalent organic framework nano-enzyme
  • Preparation method and application of bionic laccase functionalized imine covalent organic framework nano-enzyme
  • Preparation method and application of bionic laccase functionalized imine covalent organic framework nano-enzyme

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Effect test

Embodiment 1

[0069] A preparation method of bionic laccase functionalized imine covalent organic framework nanozyme, comprising the following steps,

[0070] (1) Preparation of COF-OMe precursor solution: First, 10.5 mg (0.03 mmol) of 1,3,5-tris(4-aminophenyl)benzene (TAPB) and 8.7 mg (0.045 mmol) of 2,5- Dimethoxyterephthalaldehyde (DMTP) was placed in a mixed solution containing 2 mL of dioxane, 2 mL of n-butanol and 0.5 mL of methanol, and was dissolved by ultrasonication for 10 min, which was labeled as solution A. (The molar ratio of TAPB to DMTP is 2:3)

[0071] (2) Preparation of CH / C solution: Put 0.03mmol (6.4mg) of CH / C in 0.5mL of 12mmol / L acetic acid aqueous solution, dissolve it by ultrasonication for 3min, and mark it as solution B. (The molar ratio of CH / C to TAPB is 1:1)

[0072] (3) Preparation of glutaraldehyde solution: 3.2 mL of glutaraldehyde solution with a volume fraction of 50% was placed in 16.8 mL of deionized water, mechanically stirred at 300 rpm for 3 min, an...

Embodiment 2

[0080] A preparation method of bionic laccase functionalized imine covalent organic framework nanozyme, comprising the following steps,

[0081] (1) Preparation of COF-OMe precursor solution: First, 10.5 mg (0.03 mmol) of 1,3,5-tris(4-aminophenyl)benzene (TAPB) and 8.7 mg (0.045 mmol) of 2,5- Dimethoxyterephthalaldehyde (DMTP) was placed in a mixed solution containing 2 mL of dioxane, 2 mL of n-butanol and 0.5 mL of methanol, and was dissolved by ultrasonication for 10 min, which was labeled as solution A. (The molar ratio of TAPB to DMTP is 2:3)

[0082] (2) Preparation of CH / C solution: Put 0.03mmol (6.4mg) of CH / C in 0.5mL of 12mmol / L acetic acid aqueous solution, dissolve it by ultrasonication for 3min, and mark it as solution B. (The molar ratio of CH / C to TAPB is 1:1)

[0083] (3) Preparation of glutaraldehyde solution: 3.2 mL of glutaraldehyde solution with a volume fraction of 50% was placed in 16.8 mL of deionized water, mechanically stirred at 300 rpm for 3 min, an...

Embodiment 3

[0091] A biomimetic laccase functionalized imine covalent organic framework nanozyme, comprising the following steps,

[0092] (1) Preparation of COF-OMe precursor solution: First, 10.5 mg (0.03 mmol) of 1,3,5-tris(4-aminophenyl)benzene (TAPB) and 8.7 mg (0.045 mmol) of 2,5- Dimethoxyterephthalaldehyde (DMTP) was placed in a mixed solution containing 2 mL of dioxane, 2 mL of n-butanol and 0.5 mL of methanol, and was dissolved by ultrasonication for 10 min, which was labeled as solution A. (The molar ratio of TAPB to DMTP is 2:3)

[0093] (2) Preparation of CH / C solution: Put 0.03mmol (6.4mg) of CH / C in 0.5mL of 12mmol / L acetic acid aqueous solution, dissolve it by ultrasonication for 3min, and mark it as solution B. (The molar ratio of CH / C to TAPB is 1:1)

[0094] (3) Preparation of glutaraldehyde solution: 3.2 mL of glutaraldehyde solution with a volume fraction of 50% was placed in 16.8 mL of deionized water, mechanically stirred at 300 rpm for 3 min, and marked as soluti...

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Abstract

The invention discloses a preparation method and application of bionic laccase functionalized imine covalent organic framework nano-enzyme, and the method comprises the following steps: firstly dissolving an organic ligand for synthesizing imine COFs in an organic mixed solution (solution A), and then ultrasonically dissolving a laccase active ligand in an acetic acid aqueous solution (solution B); and then, adding the solution B into the solution A to induce an amine-aldehyde condensation reaction, so as to obtain the nano-enzyme precursor for in-situ modification of the laccase active ligand on the imine COFs skeleton. On the basis of an amine-aldehyde condensation reaction, defect amino groups on the imine COFs are connected with amino acid-like molecules by utilizing glutaraldehyde, and then are coordinated with copper ions, so that the bionic laccase nano-enzyme can be obtained. The bionic laccase nano-enzyme is synthesized by taking COF-OMe as a representative, and the material has relatively high specific surface area, good structural stability and rich laccase-like active sites, and has excellent detection performance and degradation effect on phenolic compounds such as 2, 4-dichlorophenol, hydroquinone, epinephrine and the like.

Description

technical field [0001] The invention relates to the field of nanozyme biomaterials, in particular to a preparation method and application of a biomimetic laccase functionalized imine covalent organic skeleton nanozyme. Background technique [0002] Phenolic compounds are widely used in material synthesis, pesticides and disinfectants, etc., but the incomplete consumption and excessive use of such substances by natural bodies have led to the frequent detection of such pollutants in soil and natural water bodies. According to reports, when phenolic pollutants enter the human body, they can cause symptoms such as cancer, neurotoxicity, and endocrine disorders in the human body, posing a serious threat to human health. Laccase (EC 1.10.3.2) is a class of copper-containing extracellular polyphenol oxidase, which has high catalytic specificity for phenolic compounds, and can rapidly oxidize phenolic compounds into phenoxy radicals in aqueous solution. It is further degraded, and ...

Claims

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Application Information

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IPC IPC(8): B01J31/22B01J35/10G01N21/31C02F1/72C02F3/34C02F101/34
CPCB01J31/226B01J31/003C02F1/725G01N21/31C02F3/342B01J2531/16C02F2101/345B01J35/618
Inventor 赵钟兴汤颖何辉江善良胡小龙赵祯霞
Owner GUANGXI UNIV
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