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Method for producing laccase through co-culture ferment for fungus

A production method and co-cultivation technology, applied in the field of preparation of industrial enzyme preparations, can solve the problems of no patent protection in the production process, low yield of laccase, and difficulty in meeting the requirements of industrial production, etc., and achieve the safety and environmental protection of the production process, Effects that are simple and inexpensive to process

Inactive Publication Date: 2007-05-09
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of laccase in these studies is not high, it is difficult to meet the requirements of industrial production
On the other hand, there is no patent protection for the production process of fungal laccase produced by co-cultivation of microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Preparation of Trametes AH28-2 and Trichoderma ZH1 bacterial suspension (seed solution)

[0018] In potato medium (1L medium: 20% potato juice, 20g glucose, 3g KH 2 PO 4 , 1.5g MgSO 4 ·7H 2 0, 0.4g vitamin B, add 1.5% agar to make solid medium) Trametes AH28-2 and Trichoderma ZH1 preserved at 4 ℃ on the slant are respectively inoculated in high-temperature sterilized seed liquid medium (1L medium: 15g Glucose, 2.5g asparagine, 1.0g KH 2 PO 4 , 0.5g MgSO 4 ·7H 2 O, 0.1g Na 2 HPO 4 ·5H 2 O, 0.01g CaCl 2 , 0.002g CuSO 4 ·5H 2 O, 0.001gFeSO 4 ·7H 2 O, 0.0275g adenine and 0.05g vitamin B, natural pH), 28°C, 100-200rpm culture for 1-3 days, 3,000rpm homogenization for about 10s to make bacterial suspension.

[0019] 2. Preparation of co-culture fermentation medium

[0020] Fermentation enzyme production medium (1L): 10~30g carbon source, 1.0~4.0g nitrogen source, 1.0g KH 2 PO 4 , 0.5g MgSO 4 ·7H 2 O, 0.1g Na 2 HPO 4 ·5H 2 O, 0.01g CaCl 2 , 0.002g Cu...

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PUM

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Abstract

This invention discloses a method for producing laccase by fungi co-culture. The method comprises: inoculating the suspension of Trametes sp. AH28-2 into a fermentation culture medium, culturing at 22-37 deg.C for 1-4 days, adding the suspension of Trichoderma sp. ZH1, culturing for 5-10 days, and separating to obtain laccase agent with living laccase content 6,000 U / L. The culture medium is liquid culture medium, and mainly comprises certain amounts of carbon source, nitrogen source, phosphates of potassium and sodium, sulfates of copper and iron, adenine and vitamin B. The method avoids aromatic compounds and heavy metal ions to induce, thus is safe and environmentally friendly, and has high and stable laccase activity.

Description

1. Technical field [0001] The invention relates to a method for preparing an industrial enzyme preparation, in particular to a method for preparing an industrial laccase, specifically a method for producing laccase by fungal co-cultivation and fermentation. 2. Background technology [0002] Laccase (Laccase, EC 1.10.3.2) is a class of copper-containing polyphenol oxidases that catalyze the oxidation of a variety of phenolic and nonphenolic compounds with concomitant reduction of oxygen to water. Laccase widely exists in higher plants and higher fungi (especially basidiomycetes). Laccase has a wide range of substrates and high catalytic efficiency. It is widely used in biopulping and bleaching, improvement of food flavor, improvement of feed nutrition, degradation and conversion of textile dyes, softening and refinement of textile fibers, development of new drugs, and application of new biosensors. Research and development of new energy sources have potential important appli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N1/14C12R1/885
Inventor 肖亚中洪宇植张赫袁璟房伟
Owner ANHUI UNIVERSITY
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