Malachite green vestigial ELISA detection kit and usage method thereof

Abstract

The invention discloses an enzyme immunoassay of testing the bice green residues in animal derived food, which comprises an enzyme label plate covering bice green antigen, enzyme label bice green antibody working solution, bice green standard solution, substrate solution, substrate buffer solution, reaction termination solution, concentration washing liquid and sample dilute solution. The invention further discloses a method for using the immunoassay to test bice green residues, which comprises sample pretreatment, testing via the immunoassay, processing and analyzing result. The inventive immunoassay of bice green test uses direct competition enzyme-linked immunoassay adsorption analysis technique, with high sensitivity, high stability, simplified operation, reduced reaction time, reduced error caused by complex operation, reduced cost, wide application for testing samples and high practicality.

Description

technical field [0001] The invention relates to the technical field of enzyme-linked immunoassay detection, in particular to an enzyme-linked immunoassay kit for detecting malachite green residues in animal-derived foods and a method for using the same. Background technique [0002] Malachite green has potential carcinogenic, teratogenic and mutagenic effects, and its use in the aquaculture industry has not been approved by the US Food and Drug Administration (FDA); according to the European Union Act 2002 / 675 / EC, animal-derived The total amount of malachite green and colorless malachite green residues in food is limited to 2 μg / kg; Japan’s positive list also clearly stipulates that malachite green residues shall not be detected in imported aquatic products; my country’s agricultural industry standard "NY5071-2002 No Malachite green is also listed as a prohibited drug in the Guidelines for the Use of Pollution Food and Fish Medicine. [0003] In 2005, malachite green residues...

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Problems solved by technology

[0006] Retrieval of relevant literature and patents found that there are few methods and kits for malachite green immunoassay in China, and a Chinese patent "Malachite Green Indirect Competition ELISA Detection Kit in Aquatic Products" (application number 200510060995.1) was retrieved. A malachite green enzyme-linked immunosorbent assay kit, the detection principle adopted by this kit is that the enzyme plate is coated with malachite green antigen, the sample and the coated antigen compete to bind to the malachite green antibody, and the unreacted reagent is washed to remove, Then add enzyme-labeled anti-antibody reaction, wash again, add substrate for color development, and read after termination; due to the use of indirect competition ELISA detection mode, the kit has complicated operation, many steps, long detection time, high production and storage costs, Difficult to meet the needs of practical applications

[0007] In short, the detection methods used in the existing enzyme-linked immunosorbent assay kits are complex and cumbersome, and it is difficult to apply them in practice, and the existing technologies are generally poor in stability, complicated in sample pretreatment and detection steps, high in equipment requirements, and expensive. Insufficient, seriously affected the malachite green residual detection and monitoring, so the development of high stability, simple operation, low equipment requirements, cheap malachite green ELISA kit has very important economic and social significance

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