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Preparation method and applications of aptamer electrochemical sensor for detecting malachite green (MG)

A nucleic acid aptamer and biosensor technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of expensive instruments, unsuitable for large-scale detection on site, and complicated operation, and achieves fast detection speed, convenient on-site detection and operation. simple effect

Inactive Publication Date: 2014-04-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of cumbersome operation, expensive instruments, professional technicians, and not suitable for large-scale detection on site.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a nucleic acid aptamer electrochemical biosensor for detecting malachite green (MG), the steps are as follows:

[0027] (1) Add 10ul of GR-CS complex homogeneous solution to the surface of the glassy carbon working electrode dropwise, and dry overnight at room temperature;

[0028] (2) Drop 10ul of AuNPs on the surface of the modified electrode and dry at room temperature;

[0029] (3) Prepare nucleic acid aptamer solution with a concentration of 0.5mg / ml in DEPC aqueous solution, and take 10ul dropwise on the surface of the electrode, and dry it at room temperature;

[0030] (4) Use Ag / AgCl as the reference electrode, Pt as the counter electrode, and the electrochemical biosensor obtained in (3) as the working electrode, connect to the electrochemical workstation, and the scanning potential is 0.5V, and the balance is detected by the instant current method current at time.

Embodiment 2

[0032] A method for preparing a nucleic acid aptamer electrochemical biosensor for detecting malachite green (MG), the steps are as follows:

[0033] (1) Add 10ul of GR-CS complex homogeneous solution to the surface of the glassy carbon working electrode dropwise, and dry overnight at room temperature;

[0034] (2) Drop 10ul of AuNPs on the surface of the modified electrode and dry at room temperature;

[0035] (3) Use DEPC aqueous solution to prepare nucleic acid aptamer solution with a concentration of 1mg / ml, and take 10ul dropwise on the surface of the electrode, and dry it at room temperature;

[0036] (4) Use Ag / AgCl as the reference electrode, Pt as the counter electrode, and the electrochemical biosensor obtained in (3) as the working electrode, connect to the electrochemical workstation, and the scanning potential is 0.5V, and the balance is detected by the instant current method current at time.

Embodiment 3

[0038] A method for preparing a nucleic acid aptamer electrochemical biosensor for detecting malachite green (MG), the steps are as follows:

[0039] (1) Add 10ul of GR-CS complex homogeneous solution to the surface of the glassy carbon working electrode dropwise, and dry overnight at room temperature;

[0040] (2) Drop 10ul of AuNPs on the surface of the modified electrode and dry at room temperature;

[0041] (3) Prepare the nucleic acid aptamer solution with a concentration of 2mg / ml in DEPC aqueous solution, and take 10ul dropwise on the surface of the electrode, and dry it at room temperature;

[0042] (4) Use Ag / AgCl as the reference electrode, Pt as the counter electrode, and the electrochemical biosensor obtained in (3) as the working electrode, connect to the electrochemical workstation, and the scanning potential is 0.5V, and the balance is detected by the instant current method current at time.

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Abstract

The invention provides an aptamer electrochemical sensor for detecting malachite green (MG). A preparation method for the electrochemical sensor comprises the steps of: the prepared graphene-chitosan compound and nanogold are modified on an electrode surface, an aptamer is connected on the nanogold through chemical action, a target object is bound onto the electrode surface through the specific binding action of the aptamer and the target and then connected with an antibody of the target object, thus constructing the biosensor with a sandwich structure. When the detected solution contains the target object, a certain amount of target objects and corresponding antibodies can be fixed on the electrode surface, so that a sensing structure is formed. Horseradish peroxidase (HRP) is modified on the antibodies, and the hydrogen peroxide can be catalyzed to be decomposed, so that the change of an electrochemical signal can be generated, and the detection on the MG can be realized through the change of the electrochemical signal. The prepared aptamer electrochemical sensor is strong in selectivity, high in sensitivity, simple and fast to operate, and suitable for detection on the MG of aquatic products.

Description

technical field [0001] The invention belongs to the field of detection of toxic and harmful substances in nano materials and food, and provides an electrochemical biosensor for detecting malachite green, which can be used for detection of malachite green in fishery production. Background technique [0002] Malachite Green (Malachite Green, MG) is chemically called tetramethyldiaminotriphenylmethane, also known as basic green, malachite green or Chinese green, which belongs to triphenylmethane dyes. Malachite green will be very harmful to aquatic animals. Fast metabolism into colorless malachite green. Malachite green and its metabolite colorless malachite green have become a major food safety hazard. At present, malachite green is listed as a prohibited fishing drug in my country, therefore, we should establish a simple, fast and sensitive method for detecting malachite green. The current detection methods of colorless malachite green mainly include thin layer chromatograp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 王虹智黄加栋于京华刘素崔敏李杰徐伟郭玉娜
Owner UNIV OF JINAN
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