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Extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof

A technology of Rochester aerobicum and metabolites, applied in wastewater treatment, water field

Inactive Publication Date: 2009-11-18
DALIAN JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there have been no reports that Rothia spp. isolated from this type of environment have been used in the preparation of microbial flocculants, various wastewater treatment and performance improvement of activated sludge.

Method used

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  • Extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof
  • Extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028] Example 1: Extraction of Exopolysaccharide from Aerobic Strain Rothia sp.ZHT4-13

[0029] The liquid fermentation medium is: sucrose 15g; (NH 4 ) 2 SO 4 0.3g; NaCl 2g; MgSO 4 ·7H 2 O 0.5g; KH 2 PO 4 2g;K 2 HPO 4 0.5g; distilled water 1000ml; adjust the pH to 7-8 (using NaOH and H 2 SO 4 pH adjustment); 121°C, 0.1MPa, 20min autoclaving, then insert the seed solution.

[0030] A. Seed liquid culture: make nutrient agar solid slant, inoculate strain ZHT4-13, and incubate at a constant temperature of 35±1°C for 1 day; add appropriate amount (v / v=1:1) of sterile water, and vortex at 15~20°C Suspend the bacterial cells in water for 2 minutes to prepare bacterial cell seed liquid for later use.

[0031] B. Expanded fermentation culture: 0.1 ml of seed liquid was added to every 100 ml of fermentation liquid, and cultured at a constant temperature of 30-35° C., vortex shaking at 100-200 r / min for 4 days.

[0032] C. Extraction: centrifuge the fermented bacterial l...

example 2

[0033] D. Refining: add 0.5 times the volume of water to the above sample, mix well, vortex at room temperature for 1 to 5 minutes at 1000-2000r / min, add 2 times the volume of cold ethanol to precipitate, put it in a refrigerator at 4°C for 8h, and centrifuge at 9000r / min for 10min Take the precipitate and dry it in vacuum to get the refined product of microbial flocculant MBF4-13, the infrared spectrum of which is shown in the accompanying drawing. Example 2: Extraction of Exopolysaccharide from Aerobic Strain Rothia sp.ZHT4-13

[0034] The liquid fermentation medium is: sucrose 20g; (NH 4 ) 2 SO 4 0.5g; NaCl 5g; MgSO 4 ·7H 2 O 1.0g; KH 2 PO 4 5g; K 2 HPO 4 2.0g; distilled water 1000ml; adjust the pH to 7-8 (using NaOH and H 2 SO 4 pH adjustment); 121°C, 0.1MPa, 20min autoclaving, then insert the seed solution.

[0035] A. Seed liquid culture: Make a nutrient agar solid slope, inoculate strain ZHT4-13, and incubate at a constant temperature of 35±1°C for 2 days ...

example 3

[0039] Example 3: Extraction of Exopolysaccharide from Aerobic Strain Rothia sp.ZHT4-13

[0040] The liquid fermentation medium is: sucrose 25g; (NH 4 ) 2 SO 4 1.0g; NaCl 8g; MgSO 4 ·7H 2 O 1.5g; KH 2 PO 4 8g;K 2 HPO 4 3.5g; 1000ml of distilled water; adjust the pH to 7-8 (using NaOH and H 2 SO 4 pH adjustment); 121°C, 0.1MPa, 20min autoclaving, then insert the seed solution.

[0041] A. Seed liquid culture: Make a nutrient agar solid slope, inoculate strain ZHT4-13, and incubate at a constant temperature of 35±1°C for 2 days in an incubator; add appropriate amount (v / v=1:1) of sterile water, and vortex at 30-35°C Suspend the bacterial cells in water for 6 minutes to prepare bacterial cell seed liquid for later use.

[0042] B. Expanded fermentation culture: add 0.4ml of seed solution to every 100ml of fermentation broth, and culture at a constant temperature of 30-35°C and vortex shaking at 100-200r / min for 3 days.

[0043] C. Extraction: Centrifuge the ferment...

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Abstract

The invention relates to an extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof. An aerobic strain ZHT4-13 is separated from adhesive sludge of wild Ruditapes philippinarums in the Bohai sea offshore of China and is identified as a Ruthia sp. The aerobic strain is treated by seed culture and amplification culture, and a fermentation broth of the aerobic strain is precipitated with ethanol and separated centrifugally to obtain the extra-cellular polysaccharide MBF4-13. The extra-cellular polysaccharide MBF4-13 is measured to have high flocculation activity, the FR value to kaolin reaches over 80 percent, and the removal rate to hexavalent chromium ions reaches 69.3 percent; the extra-cellular polysaccharide MBF4-13 has high activity to decolorize high-concentration wastewater, and the decolorization ratios to methylene blue, ink blue, malachite green and crystal violet reach 86.11 percent, 99.49 percent and 97.84 percent; and the extra-cellular polysaccharide MBF4-13 plays a role of obviously improving the performance and the structure of activated sludge. The extra-cellular polysaccharide has potential value of developing a novel microbial flocculant.

Description

technical field [0001] The invention belongs to the field of water and waste water treatment. It involves flocculation and precipitation of suspended impurities, and is a flocculant used for sedimentation and separation; in particular, it relates to a source of microbial flocculant-producing bacteria, a method for fermentation and cultivation of bacterial strains, a preparation method for the flocculant MBF4-13 produced by it, and the flocculant Uses for flocculation, sedimentation and decolorization in wastewater treatment. Background technique [0002] Flocculants are one of the commonly used agents in water treatment technology. In recent years, the most widely used traditional high-molecular polymer flocculants have been found to have potential problems that threaten human health and destroy the ecological environment. Research on new environmentally friendly and pollution-free flocculants has become a hot issue in water treatment research. Most of them are microbial f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04C02F1/56C02F1/62C02F3/12C12R1/01
CPCY02W10/10
Inventor 穆军朱秀华高琦张翼顾晓洁
Owner DALIAN JIAOTONG UNIVERSITY
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