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Method for preparing microbial flocculant through enteromorpha hydrolysate fermentation

A microbial flocculant and hydrolyzate technology, applied in the field of microbial flocculant preparation, can solve the problem of high cost of raw materials, achieve the effect of simple production mode, high flocculation activity, and food saving

Inactive Publication Date: 2015-11-11
青岛美能达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problem of high cost of raw materials in the process of microbial preparation of flocculant against the deficiencies of the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of liquid strains

[0027] (1) Cultivation of slant strains: inoculate DS16 strains on slant medium, and incubate at 28°C for 36 hours.

[0028] The slant medium consists of 3 g / L beef extract, 8 g / L peptone, 7 g / L liquid sugar, 3 g / L NaCl, and pH=7.0.

[0029] (2) Liquid strain culture: Put 50mL of shake flask culture medium in a 250mL Erlenmeyer flask, inoculate a loop of slanted strains, and incubate at 100r / min, 28°C for 12h to obtain liquid strains.

[0030] The shake flask medium consists of glucose 8g / L, peptone 6g / L, yeast extract 0.3g / L, K 2 HPO 4 3g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O0.3g / L, pH=7.

[0031] 2. Fermentation

[0032] (1) Wash the fresh Enteromorpha to remove salt, sediment and algae, add appropriate amount of water, crush it with a pulverizer, add it to the hydrolysis tank, add water to make the ratio of material to water 1:20, add sulfuric acid to make the acidity 0.5% (w / w). Introduce high-temperature steam, raise the tem...

Embodiment 2

[0040] 1. Preparation of liquid strains

[0041] Cultivation of slant strains: Inoculate DS16 strains on the slant and incubate at 30°C for 36 hours;

[0042] The slant medium consists of beef extract 6g / L, peptone 12g / L, liquid sugar 12g / L, NaCl 6g / L, pH=7.2;

[0043]Cultivation of liquid strains: put 50mL of shake flask culture medium in a 250mL Erlenmeyer flask, inoculate a slant strain of an inoculation loop, and incubate at 120r / min, 30°C for 12h;

[0044] The shake flask medium consists of glucose 1210g / L, peptone 12g / L, yeast extract 0.8g / L, K 2 HPO 4 8g / L, KH 2 PO 4 7g / L, MgSO 4 ·7H 2 O0.9g / L, pH=7;

[0045] 2. Fermentation

[0046] (1) Wash the fresh Enteromorpha to remove salt, sediment and algae, add an appropriate amount of water and crush it with a pulverizer, then add it to the hydrolysis tank, add water to make the ratio of material to water 1:30, add sulfuric acid to make the acidity 5 %(w / w). Introduce high-temperature steam, raise the temperature to...

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Abstract

The invention discloses a method for preparing a microbial flocculant through enteromorpha hydrolysate fermentation. The method comprises the steps of preparation of liquid strain, fermentation and product aftertreatment. The fermentation process comprises the steps that 1, fresh enteromorpha is cleaned, salt, sediment and unwanted algae are removed, then the fresh enteromorpha is smashed and added to a hydrolysis tank, water is added to regulate acidity, alkali is added for neutralization, filter pressing and clarification are conducted, and then a hydrolysate is obtained; 2, the hydrolysate is diluted till the sugar concentration of reducing sugar is 15-30 g / L, fermentation liquor is prepared and added to a fermentation tank, and high-temperature sterilization and cooling are conducted; 3, a seed solution is inoculated into the fermentation tank for fermentation, and fermentation is ended after the content of residual sugar in the fermentation liquor is reduced to the level below 3 g / L. According to the method, enteromorpha is used as the raw material, microbial flocculant fermentation is directly conducted after acid hydrolysis filtration, the production method is simple, production cost is low, grain is saved, and waste is turned into wealth.

Description

technical field [0001] The invention relates to the field of microbial flocculant preparation, in particular to a method for preparing microbial flocculant by fermentation of enteromorpha hydrolyzate. Background technique [0002] Enteromorpha is a large green algae belonging to the Ulvaceae family. The common types include Enteromorpha barbata and Enteromorpha permanensis. Enteromorpha is distributed in the northern and southern sea areas of my country, and Enteromorpha grows rapidly, with a daily multiplication rate of 12.7%. It can form a very considerable biomass in a short period of time, and it is a resource organism that needs to be developed urgently. Enteromorpha contains a lot of nutrients. Enteromorpha contains about 50% polysaccharides and about 12% protein. After acid hydrolysis, Enteromorpha can produce sugars such as glucose, xylose, and rhamnose. These substances can be well utilized by microorganisms. Due to the pollution and eutrophication of seawater in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12R1/265
Inventor 姜国辉王强李守富顾玉祥
Owner 青岛美能达生物科技有限公司
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