57 results about "Anaeroarcus" patented technology

The invention relates to an extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof. An aerobic strain ZHT4-13 is separated from adhesive sludge of wild Ruditapes philippinarums in the Bohai sea offshore of China and is identified as a Ruthia sp. The aerobic strain is treated by seed culture and amplification culture, and a fermentation broth of the aerobic strain is precipitated with ethanol and separated centrifugally to obtain the extra-cellular polysaccharide MBF4-13. The extra-cellular polysaccharide MBF4-13 is measured to have high flocculation activity, the FR value to kaolin reaches over 80 percent, and the removal rate to hexavalent chromium ions reaches 69.3 percent; the extra-cellular polysaccharide MBF4-13 has high activity to decolorize high-concentration wastewater, and the decolorization ratios to methylene blue, ink blue, malachite green and crystal violet reach 86.11 percent, 99.49 percent and 97.84 percent; and the extra-cellular polysaccharide MBF4-13 plays a role of obviously improving the performance and the structure of activated sludge. The extra-cellular polysaccharide has potential value of developing a novel microbial flocculant.

The invention relates to the field of micro-alga biotechnologies, in particular to a symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving the quality of grease and the stability of culture systems and application of the symbiotic bacterium system. The symbiotic bacterium system comprises the scenedesmus obliquus and symbiotic bacteria. The symbiotic bacteria include one type or a plurality of types of brevunmdimonas sp., rhizobium sp., pseudomonas sp. and acidovorax sp. The symbiotic bacterium system and the application have the advantages that the symbiotic bacteria and mixed floras of the symbiotic bacteria are applied to scenedesmus obliquus culture procedures, extracellular substances and oxygen which are generated by the scenedesmus obliquus can be consumed by each single type of symbiotic bacteria and the mixed floras, carbon dioxides can be generated to be utilized by the scenedesmus obliquus, micro-environments favorable for growth of the scenedesmus obliquus can be created, and accordingly substance exchange and metabolism of the scenedesmus obliquus, the symbiotic bacteria and the mixed floras can be promoted.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

A method for comparing the variable reactivity of multiple, differentially mutated copies of 16S subsequences found in a number of ribosomal RNA operons of a single bacterial cell is described. The application of this method for distinguishing between closely related organisms, such as the genera Escherichia and Shigella, and between species of Shigella including S. boydii, S. dysenteriae, S. flexneri, and S. sonnei using nucleic acid probes is also presented.

The invention belongs to the field of soil environment repairing, further relates to organic pollutant electric repairing and microbial repairing synergistic treatment technology and particularly relates to a functional strain applicable to petroleum polluted soil repairing in the positive column of a direct current electric field. The strain is Variovorax JWLB3 (Variovorax sp.JWLB3), is preservedin the China Center for Type Culture Collection on October 11, 2019, and has a preservation access number of CCTCC NO:M 2019811. The Variovorax JWLB3 is used for combining with the electric repairingtechnology and repairing petroleum polluted soil in each area applied by the direct current electric field, especially the positive column of the electric field. When a relatively proper water containing condition is kept, the functional strain can keep relatively high bioactivity under the conditions of high acidity, high soluble salt ions, high chlorine content, high redox potential and strongcurrent intensity of the electrically repaired positive column, and keep relatively good petroleum hydrocarbon degradation performance.

The invention relates to the field of microorganisms, and particularly discloses a preparation method and application of a low-temperature efficient corn straw degrading bacterial agent. The bacterial agent is prepared from two single-plant bacteria, wherein the two single-plant bacteria are Pseudomonas sp. and Acinetobacter sp. and the preservation numbers are CGMCC No.20521 and CGMCC No.20522 respectively. According to the preparation method, the specific-composition bacteria genus is used as a bacteria source sample, a culture medium adapted to the bacteria source sample is used to perform fermentation propagation, the bacterial liquid fermented under the optimal fermentation condition cooperates with a suitable carrier and a protective agent, and a freeze drying technology is adopted to prepare the powdery bacterial agent. The bacterial agent can degrade corn straw under the low temperature condition, the preparation method is simple, the cost is low, the production period is short, and the problems that agricultural production is inconvenient due to the fact that straw cannot be degraded under the low-temperature condition after straw returning to the field in the north can be solved.

The invention relates to the field of microorganisms, and particularly discloses a low temperature efficient corn stalk degradation composite microbial system and an application thereof. The composite microbial system comprises bacteria and fungi. The bacteria comprise a Cellvibrio bacterial genus, an Azospira bacterial genus, an Arcobacter bacterial genus, an Azonexus bacterial genus, a Clostridium bacterial genus and a Paludibacter bacterial genus. The fungi comprise a Lotharella bacterial genus and a Trichosporon bacterial genus. According to the low temperature efficient corn stalk degradation composite microbial system and the application thereof, the bacterial genera with specific components are used as bacterium source samples, culture media adaptive to the system are adopted, the composite microbial system which is composed of the specific bacterial genera is screened and acclimated through enrichment culture, subculture, enzyme production living bacterium culture and corn stalk degradation culture, corn stalks can be efficiently degraded under a low temperature condition through the system, and the defect that an existing microorganism bacterium agent can only degrade the crop stalks under a medium-high temperature condition is overcome.

A person's intestinal (gut), oral or skin microbiota is modified using specific combinations of pre-biotics, pro-biotics and / or anti-biotics to establish a defined microbiota that can treat and / or reduce the likelihood that individuals will experience various diseases, including cancer. The employment of various bacteria, whether in particular combinations or after being modified using CRISPR-type systems, leads to improved outcomes when checkpoint inhibitors are used to treat various forms of cancer. One embodiment is directed to a method for reducing the likelihood of developing cancer by providing in the gut of an individual a population of beneficial bacteria selected from the group consisting of Lactobacillus species. The level of Roseburia and Faecalibacterium prausnitzii, and / or Akkermansia muciniphila bacteria are increased in the individual's gut microbiome such that when an individual is administered an immune checkpoint inhibitor, its function is enhanced due to the presence of the bacterial population.

The invention discloses a TaqMan probe quantitative detection method for detecting pseudomonas putida and a corresponding kit. Specific gene detection is ingeniously applied to distinguish pseudomonasputida from strains or insect species of other species, and accurate bacterial genus information is obtained through comprehensive judgment. Compared with the existing mainstream detection kit, the kit provided by the invention for detecting the pseudomonas putida has the advantages of high sensitivity, rapidness, convenience, high specificity, rigorous and accurate judgment and the like, and hashigh application prospect and market value.

The invention discloses a polycyclic aromatic hydrocarbon degrading bacterium, a screening method and an application thereof, and relates to a marine petroleum hydrocarbon degrading bacterium. In order to transform Alcanivorax xenomutans 45II-AX, the transformed Alcanivorax xenomutans is a gram-negative bacterium of Alcanivorax, the biological characteristics are non-fermentative, obligate aerobic, and the thallus form is bacillus-free, the colony is round, yellow and opaque, the surface is smooth and moist, the edge is regular, the bacterium has no halo, the center is convex, the diameter is2-3 mm, and the optimum growth condition is pH = 7.0-8.5, and the temperature is 25-28 DEG C; the bacterium can be applied to degrading organic compounds. After identification, the 16S rDNA gene sequence of the transformed Alcanivorax xenomutans is identified in the model strain Alcanivorax xenomutans JC109(T), with a similarity of 99.93%.

A polycyclic aromatic hydrocarbon degrading bacterium and its screening method and application relate to marine petroleum hydrocarbon degrading bacteria. In order to transform the heterologous alcanivorax (Alcanivorax xenomutans) 45II-AX, transform the heterologous alcanivorax into a Gram-negative strain of the genus Alcanivorax, with biological characteristics of non-fermentative, obligate aerobic, The shape of the bacteria is non-bacillus, the colony is round, yellow and opaque, the surface is smooth and moist, the edges are regular, no halo, the center is raised, the diameter is 2-3mm, the optimum growth conditions are: pH=7.0-8.5, temperature 25 ~28°C; can be used in the degradation of organic compounds. It was identified that the 16S rDNA gene sequence of a transformed heterologous alcanivorax was 99.93% similar to the type strain Alcanivorax xenomutans JC109(T).

The invention discloses a method for treating sewage by enrichment culture of anaerobic ammonium oxidation bacteria, and relates to a method for treating sewage, an integrated semi-nitrification anaerobic ammonium oxidation reactor is used in the method, and a water inlet tank is connected with a water inlet at the bottom of a reactor main body through a peristaltic pump; a filler frame is arranged in the reactor main body, an aeration head is arranged at the bottom in the reactor, the aeration amount of the aeration head is controlled by an aeration pump, and a pH online tester and a heatingrod are arranged at the middle upper part; the reactor main body is connected with an alkali barrel, and alkali is provided for the interior of the reactor through a liquid supplementing pump; and thereactor main body is connected with a sedimentation tank through a water outlet. When the system is used for sewage treatment, the device needs to be started firstly, then the ammonia nitrogen waterinlet load is increased, and humic acid is added in the experimental stage. According to the method, a proper amount of humic acid is added into the integrated semi-nitrification anaerobic ammonia oxidation reactor, and the proportion of anaerobic ammonia oxidation bacteria is increased, so that anaerobic ammonia oxidation bacteria are enriched, the denitrification capacity is improved, and the ammonia nitrogen removal rate can reach 95%.

A person's intestinal (gut), oral or skin microbiota is modified using specific combinations of pre-biotics, pro-biotics and / or anti-biotics to establish a defined microbiota that can treat and / or reduce the likelihood that individuals will experience various diseases, including cancer. The employment of various bacteria, whether in particular combinations or after being modified using CRISPR-type systems, leads to improved outcomes when checkpoint inhibitors are used to treat various forms of cancer. One embodiment is directed to a method for reducing the likelihood of developing cancer by providing in the gut of an individual a population of beneficial bacteria selected from the group consisting of Lactobacillus species. The level of Roseburia and Faecalibacterium prausnitzii, and / or Akkermansia muciniphila bacteria are increased in the individual's gut microbiome such that when an individual is administered an immune checkpoint inhibitor, its function is enhanced due to the presence of the bacterial population.

The invention provides an in-situ remediation method for petroleum-contaminated soil. The in-situ remediation method comprises a step of applying microorganisms to soil and a step of conducting plantcultivation and maintenance on the soil. Preferably, the microorganisms are composite microorganisms containing dominant bacteria including pseudomonas, planoeoccus, alcanivorax, mycobacteria, carnobacterium, paracoccus, kurthia, luteimonas and sphingobium; and the cultivated plants are preferably reeds and alfalfa. According to the in-situ remediation method provided by the invention, the petroleum-contaminated soil is subjected to in-situ remediation treatment through a biological enhancement-plant purification combined process; and according to the method, petroleum pollutants in the soil can be rapidly and effectively reduced or even removed, remediation cost is low, secondary pollution is avoided, operation is safe, and the method is suitable for in-situ remediation of soil contaminated by petroleum and / or polycyclic aromatic hydrocarbons.

The invention discloses application of Callerya speciosa endophyte RH5 in promotion of the growth of Callerya speciosa. The classification name of Callerya speciosa endophyte RH5 is Burkhoideria sp. RH5, and is submitted to the Guangdong Province Microbial Collection on May 29th, 2020, and the preservation number is GDMCC NO: 61030. The endophyte RH5 is added during the growth of Callerya speciosa, and the strain is capable of promoting the growth of Callerya speciosa and improving the yield and quality of the Callerya speciosa medicinal material.

The invention discloses (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow, and relates to the biotechnology field of edible fungi. The (Bosea sp.)Y4 is collected in the China Center for Type Culture Collection with a collection number CCTCC NO:M 2019634, wherein a collection date is 16th, August, 2019, and a collection address is Wuhan University, China. The (Bosea sp.) Y4 is used for accelerating the Hypsizygus marmoreus(Peck)H .E .Bigelow to grow. Through bacteria solution culture, cultivation application and fruiting management, the Hypsizygus marmoreus(Peck)H .E .Bigelow in which the (Bosea sp.) Y4 is added is characterized in that a hypha growth rate is increased, the diameter of a sporocarp pileus is increased, a stipe is long, time for a bottle to be full is shortened and a bottle yield is increased. The growth rate of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be effectively improved, the production period of the Hypsizygus marmoreus(Peck)H .E .Bigelow is shortened, the yield of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved, a product is guaranteed to be environmentally-friendly, healthy and free frompollution, and the production efficiency and the social benefit of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved.

The invention belongs to the field of soil environment bioremediation, and further develops a bacteria-enzyme composite preparation for biodegradation of petroleum hydrocarbon pollutants in petroleumhydrocarbon contaminated soil, so as to realize efficient and rapid petroleum hydrocarbon contaminated soil bioremediation. The composite preparation is a microbial inoculum with petroleum hydrocarbondegradation capability and an enzyme agent obtained by crushing the microbial inoculum; the microbial inoculum is a culture solution which is obtained by carrying out fermentation culture on Variovorax sp. JWLC1 at the temperature of 30 + / -1 DEG C until the OD value is 1.5-2.5, wherein the volume ratio of the fermentation culture solution in the microbial inoculum to the fermentation culture solution in the enzyme agent is 1: 1 to 1: 3. The bacteria-enzyme composite preparation disclosed by the invention remarkably prolongs the microbial efficient degradation process of the petroleum hydrocarbon and improves the bioremediation efficiency of the petroleum hydrocarbon contaminated soil.

The invention relates to a mixed bacteria producing biosurfactant and a screening method thereof. The mixed bacteria is composed of three strains, named Pseudomonas stutzeri (Pseudomonas stutzeri) and the preservation numbers are CCTCC AB 205091, Composition of strains named Nocardioides ginsengagri with preservation number CCTCC S2013441 and Bacillus licheniformis with preservation number CCTCC AB 205141. 1. Activate and cultivate the selected oil production strains to be screened, conduct preliminary screening on the activated and cultured oil production strains to be screened by the blood plate method to obtain the primary screening fermentation liquid, and use the oil discharge ring method to re-screen the primary screening fermentation liquid after filtering. The mixed bacteria combines the advantages of the three strains and can produce biosurfactants used in oil recovery. The screening method of the mixed bacteria is widely applicable and effectively reduces the screening cost. In addition, the screening method has high accuracy and selectivity. Strong, convenient and easy-to-operate process, and compared with the prior art, the cycle is shorter.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has L- theonine productivity and has been modified to enhance an activity of aspartate aminotransferase.

The invention belongs to the technical field of wastewater treatment, and particularly relates to a method for promoting proliferation of anaerobic ammonium oxidation bacteria. The method for promoting the proliferation of the anaerobic ammonium oxidation bacteria comprises the step of adding an accelerant vitamin C. According to the invention, vitamin C is added to serve as an accelerant to promote proliferation of anaerobic ammonium oxidation bacteria, can improve the denitrification effect of the anaerobic ammonium oxidation bacteria, more importantly shortens the generation time of the anaerobic ammonium oxidation bacteria, and increases the proliferation speed of the bacteria.

The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

The invention discloses a myxobacterium H56D21 that preys on plant pathogenic bacteria and its application, belonging to the field of microbial technology and biological control. Analyze the ANI value and the dDDH value of bacterial strain H56D21 and similar type bacterial strains, the result shows that the H56D21 bacterial strain of the present invention is a Hypocystis ( Hyalangium ) of new species. of the present invention Hyalangium sp.H56D21, was deposited in Guangdong Microbial Culture Collection Center on November 17, 2020. The address is 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, postcode: 510070, and the deposit number is: GDMCC No: 61294, which can prey on plant pathogenic bacteria and has broad-spectrum predation characteristics, and can be used for biological control of plant diseases.

The present invention relates Pseudomonas strains and consortia thereof that are useful in protecting plants against microbial plant diseases caused by pathogens such as Ralstonia, Clavibacter, Erwinia, Curtobacterium, Fusarium, Phytophthora and Helminthosporium. The Pseudomonas strains were selected on the basis of their antagonistic abilities against plant pathogens such as production of antimicrobial compounds, direct inhibition of growth of plant pathogens, competition of carbon or nitrogen sources and endophytic features such as anaerobic growth on nitrate as electron acceptor and growth on arabinose as carbon source. The invention further relates to compositions comprising the strains or consortia of the invention, preferably lyophilized compositions, and to methods wherein they are used in protecting a wide variety of plants against a wide variety of microbial plant diseases.

The invention discloses a fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting toxin-producing neisseria meningitidis and a corresponding kit. According to the invention, specific gene detection is ingeniously applied to distinguish neisseria meningitidis from other cocci and to distinguish toxic and non-toxic cocci, and accurate bacteria genus information is obtained through comprehensive judgment. Compared with an existing mainstream detection kit, the kit for detecting the toxin-producing neisseria meningitidis has the advantages of being high in sensitivity, beingrapid and convenient to use, being good in specificity, being rigorous and accurate in judgment and the like, and has good application prospects and market value.

The invention discloses a marker for predicting attack and severity of hereditary angioedema (HAE) and application of the marker. The marker for predicting the HAE attack is the relative abundance of 20 bacterial genus of the throat of a patient, after the relative abundance data of the 20 bacterial genus are input into the random forest model, the obtained score is greater than or equal to 0.5, the HAE attack risk is high, and the obtained score is lt; and the attack risk of the HAE is low. The marker for predicting the severity of the HAE attack is the relative abundance of Bacteroides bacteria in the throat of a patient. The invention finds that the throat flora is closely related to the attack and severity of the HAE for the first time, establishes a set of marker and risk early warning model for the acute attack of the HAE based on the throat flora and an evaluation system for the severity of the attack, can assist in clinically predicting the acute attack of the HAE in advance, provides a marker for future flora transplantation of a patient, and has a good application prospect. The important significance is realized on reducing the occurrence rate of the HAE acute edema and improving the treatment rate.

The invention discloses a fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting enterocytozoon hepatopenaei of prawns and a corresponding kit. Specific gene detection is ingeniously applied to distinguish prawn enterocytozoon hepatopenaei from other bacterial genera, and accurate bacterial genus information is obtained through comprehensive determination. Compared with an existing mainstream detection kit, the kit for detecting prawn enterocytozoon hepatopenaei has the advantages of being high in sensitivity, rapid, convenient to use, good in specificity, rigorous and accurate in judgment and the like, and has good application prospects and market value.