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Symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving quality of grease and application of symbiotic bacterium system

A technology of Scenedesmus obliquus and symbiotic bacteria, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of pollution, collapse of culture system, low yield and so on

Active Publication Date: 2016-02-03
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current yield of this species of algae is low, and its optimum culture pH is 7-8. Microalgae at neutral pH are extremely susceptible to contamination by foreign species, leading to the collapse of the culture system.

Method used

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  • Symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving quality of grease and application of symbiotic bacterium system
  • Symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving quality of grease and application of symbiotic bacterium system
  • Symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving quality of grease and application of symbiotic bacterium system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening and identification of symbiotic bacteria of Scenedesmus obliquus

[0047] The screening and identification method of Scenedesmus symbiotic bacteria in the present invention includes the following steps:

[0048] 1. Purification

[0049] The Scenedesmus obliquus used in the present invention can be any strain of Scenedesmus obliquus known in the art. The Scenedesmus obliquus used in this example came from a freshwater lake and was continuously subcultured in the laboratory for three years.

[0050] Inoculate 5 mL of Scenedesmus obliquus culture solution into 1 L of fresh sterilized BG11 medium, culture for 14 days, and take the 4th and 14th day culture solutions of Scenedesmus obliquus, respectively. Dilute with ultrapure water 10 4 -10 6 Fold, take 100 microliters respectively to spread in YPD and inorganic salt medium, put them in the incubator, and incubate at 27°C for 3 days. The single colonies formed in different plates were streaked and purified.

[0...

Embodiment 2

[0180] Example 2 The effect of co-cultivation of Scenedesmus obliquus, symbiotic bacteria and their mixed flora on the growth of Scenedesmus obliquus

[0181] The selected single symbiotic bacteria and their mixed flora (Table 1) and Scenedesmus obliquus were mixed and inoculated at a ratio of 1:3 (number / number) to 1L sterile (121℃, 20 minutes) BG11 A co-cultivation system of Scenedesmus obliquus and bacteria was constructed in a cylindrical photobioreactor (50 cm high and 6.5 cm diameter) with medium. The inoculation conditions are: the inoculated Scenedesmus obliquus and symbiotic bacteria are taken from the logarithmic growth phase; the culture temperature is 22-30℃, and the optimal is 27℃; the aeration head at the bottom of the reactor is filled with air and carbon dioxide (air 0.2 L / min, carbon dioxide 5mL / min), the light intensity is 120μmolm -2 s -1 , Continuous light.

[0182] The experimental results show (as shown in Table 2, image 3 ), after 10 days of cultivation, in...

Embodiment 3

[0186] Example 3 Effect of co-cultivation of Scenedesmus obliquus with symbiotic bacteria and its mixed flora on oil production of Scenedesmus obliquus

[0187] The selected single symbiotic bacteria and their mixed flora (Table 1) and Scenedesmus obliquus were mixed and inoculated at a ratio of 1:3 (number / number) into 1L sterile (121℃, 20 minutes) BG11 culture In a cylindrical photobioreactor (50 cm high and 6.5 cm diameter), a co-culture system of Scenedesmus obliquus and bacteria was constructed. The inoculation conditions are: the inoculated Scenedesmus obliquus and symbiotic bacteria are taken from the logarithmic growth phase; the culture temperature is 22-30℃, and the optimal is 27℃; the aeration head at the bottom of the reactor is filled with air and carbon dioxide (air 0.2 L / min, carbon dioxide 5mL / min), the light intensity is 120μmolm -2 s -1 , Continuous light.

[0188] The oil analysis method used is as follows: Take Scenedesmus at 3d, 6d, 9d during the culture proce...

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Abstract

The invention relates to the field of micro-alga biotechnologies, in particular to a symbiotic bacterium system for increasing biomasses of scenedesmus obliquus and improving the quality of grease and the stability of culture systems and application of the symbiotic bacterium system. The symbiotic bacterium system comprises the scenedesmus obliquus and symbiotic bacteria. The symbiotic bacteria include one type or a plurality of types of brevunmdimonas sp., rhizobium sp., pseudomonas sp. and acidovorax sp. The symbiotic bacterium system and the application have the advantages that the symbiotic bacteria and mixed floras of the symbiotic bacteria are applied to scenedesmus obliquus culture procedures, extracellular substances and oxygen which are generated by the scenedesmus obliquus can be consumed by each single type of symbiotic bacteria and the mixed floras, carbon dioxides can be generated to be utilized by the scenedesmus obliquus, micro-environments favorable for growth of the scenedesmus obliquus can be created, and accordingly substance exchange and metabolism of the scenedesmus obliquus, the symbiotic bacteria and the mixed floras can be promoted.

Description

Technical field [0001] The present invention relates to the technical field of microalgae biology. Specifically, the present invention relates to a symbiotic bacteria system and its application for improving the biomass, oil quality and stability of the culture system of Scenedesmus obliquus. Background technique [0002] With the ever-decreasing fossil energy sources and the serious environmental problems it brings, finding alternative energy sources has become an urgent need. Microalgae is considered to be the most promising third-generation biomass energy to replace fossil energy, but the high cost of cultivation has become a bottleneck for its development. Large-scale outdoor cultivation is an important means to reduce the cost of microalgae cultivation, but the biomass of outdoor cultivation of microalgae is very low and susceptible to contamination by other species. Especially for oil-producing microalgae with a neutral pH, the pollution of other species can easily lead to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/12C12P7/64C12R1/89C12R1/01C12R1/41
Inventor 丛威王瑞民颜成虎薛升长
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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