The invention discloses a method for increasing PUFA (
polyunsaturated fatty acid) relative content of soft parts of
ruditapes philippinarum. The method comprises the following steps: collecting
ruditapes philippinarum with undamaged body surfaces and shell length of 2.5-4.5 cm, transferring the
ruditapes philippinarum into a room for room-temperature temporary rearing in temporary rearing
seawater with
salinity being 26-32 and pH value being 7.8-8.3, changing the total amount of water once every day in the temporary rearing period, feeding twice every day, performing continuous oxygenating, setting the culture capacity to be 20-30 kg / m<3>, after one week of temporary rearing, putting the ruditapes philippinarum into a temperature-controllable
water circulation tank, carrying out gradient low-
temperature treatment on the ruditapes philippinarum, reducing the culture temperature at the speed of 2-3 DEG C / d by taking the
seawater temperature at
room temperature as the initial temperature, when the
water body temperature is 4 DEG C or above, feeding the ruditapes philippinarum with unicellular
algae or
chlorella algae paste every day with the feeding amount of 80-120 g / m<3>, and meanwhile, changing the total amount of water once every day; when the temperature of the
water body is reduced to 4 DEG C or below, stopping feeding, meanwhile, performing continuous oxygenating, picking out dead individuals in time, changing water once every 3-5 days, when the temperature of the
water body is reduced to 0 DEG C, stopping cooling, maintaining the temperature of 0 DEG C for one week or more, and changing water once a week.