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Preparation method and application of anti-oxidation Venerupis philippinarum enzymatic extract

A technology for oxidizing clams enzymes and extracts, which is applied in the directions of medical preparations, antidote, and drug combinations containing active ingredients to achieve a good scavenging effect.

Inactive Publication Date: 2013-01-02
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Modern studies have proved that clam extract has the effects of improving immunity, anti-tumor, anti-atherosclerosis, and antibacterial, but for the hydrolysis of clam with papain, the research on the related hydrolysis process of obtaining enzymatic hydrolyzate with antioxidant activity Not yet reported

Method used

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  • Preparation method and application of anti-oxidation Venerupis philippinarum enzymatic extract
  • Preparation method and application of anti-oxidation Venerupis philippinarum enzymatic extract
  • Preparation method and application of anti-oxidation Venerupis philippinarum enzymatic extract

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Embodiment Construction

[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0021] 1 material

[0022] Flower clams, purchased from Zhoushan market, were identified as Ruditapes philippinarum.

[0023] 2 methods

[0024] 2.1 Orthogonal experiment

[0025] Select papain for use, consider A (temperature), B (time), C (enzyme amount) and D (PH) four factors, each factor selects three levels and carries out the orthogonal experiment of four factors three levels, compares papaya The degree of hydrolysis and hydroxyl radical scavenging rate of protease under different hydrolysis conditions can determine the optimal hydrolysis conditions of papain. The hydrolysis process is as follows: wash the clams → remove the shell and take the meat → homogenate → adjust the pH value with 0.5mol / L NaOH solution → keep warm → add enzyme hydrolysis → inactivate the enzyme (90°C, heat for 15min) → centrifuge (5000r / min, 20min) →Constant...

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Abstract

A preparation method of anti-oxidation Venerupis philippinarum enzymatic extract includes preparing sample by cleaning Venerupis philippinarum, shelling to obtain meat, homogenizing, and adding deionized water at the ratio of material to liquid of 1:2-1:3; and regulating pH to 6.8-7.3, adding papain 1500-2000 u / g, performing enzymolysis at 42-47 degrees for 2-4 h, inactivating at 90-95 degrees, centrifuging, and collecting supernatant to obtain enzymolysis product. The invention also discloses an application of enzymatic extract in oxidation resistance activity. Compared with prior art, the invention obtains a perfect process via control on pH value, enzyme amount, temperature and enzymolysis time, and has perfect effect of removing a plurality of free radicals.

Description

technical field [0001] The invention relates to a preparation method of clam enzymolysis extract, which can be used for anti-oxidation. Background technique [0002] Superoxide anion (O 2- ), hydrogen peroxide (H 2 o 2 ), free radicals such as peroxide radicals (ROO) and hydroxyl radicals (-OH) are the products of the normal metabolism of organisms. When free radicals are produced too much or cannot be effectively eliminated after generation, they can cause cell membrane, DNA and protein activity. The damage of human body can cause a variety of human diseases such as arteriosclerosis, stroke, Alzheimer's disease, obesity, cataract and liver disease. Therefore, in recent years, the separation, extraction and action mechanism of antioxidant active substances have become a research hotspot. Up to now, a variety of antioxidant active peptides have been obtained from various food proteins such as dairy products, eggs, soybeans, meat, and corn by using proteolysis methods, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61P39/06A61K35/618
Inventor 杨最素丁国芳许新建杨永芳曾军勇黄芳芳郁迪
Owner ZHEJIANG OCEAN UNIV
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