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A strain of marine cryobacteria and method for preparing flocculant by using it

A technology of flocculant and bacillus, applied in the field of water treatment, can solve the problem of low flocculation activity, achieve strong flocculation effect, stable performance, and reduce the use of ethanol

Active Publication Date: 2020-07-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low flocculation activity in the preparation of extracellular polysaccharide flocculants by microorganisms at the present stage, the purpose of the present invention is to provide a strain of marine Pyrobacterium that can secrete exopolysaccharides with flocculation. It is separated and purified from sludge liquid, and the flocculant prepared from the exopolysaccharide secreted by the marine Pyrobacterium strain has high flocculation activity, strong flocculation ability and stable performance

Method used

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  • A strain of marine cryobacteria and method for preparing flocculant by using it

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Experimental program
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Effect test

Embodiment 1

[0031] A method for preparing a flocculant using the marine cryobacter strain GHF2 includes the following steps:

[0032] (1) Inoculate the strain on the solid medium and cultivate at 18°C ​​for 36 hours, then add sterile water to make the strain liquid, the volume ratio of sterile water to solid medium is 1.0:1;

[0033] (2) Inoculate the bacterial seed liquid into the liquid culture medium, the inoculation concentration is 0.8mL bacterial seed liquid / 100mL liquid medium, ferment culture 3 days at 18℃, the shaker speed is 160r / min, make the fermentation bacterial liquid;

[0034] (3) Add a flocculation carrier to the fermentation broth, adding 5g / 100mL fermentation broth, and then continue to ferment and culture for 1 day to obtain the raw material solution. The flocculation carrier is sandy soil, preferably the natural sandy soil for cultivating clam clams in the Philippines. Sterilize for use after sieving through the mesh screen;

[0035] (4) Add ethanol to the raw material liquid...

Embodiment 2

[0038] A method for preparing a flocculant using the marine cryobacter strain GHF2 includes the following steps:

[0039] (1) Inoculate the strain on the solid medium and cultivate for 42 hours at 20°C, then add sterile water to make the strain liquid, the volume ratio of sterile water to solid medium is 1.25:1;

[0040] (2) Inoculate the bacterial seed liquid into the liquid culture medium, the inoculation concentration is 1.1mL bacterial seed liquid / 100mL liquid medium, ferment culture at 20℃ for 4 days, the shaker rotation speed is 180r / min, make the fermentation bacterial liquid;

[0041] (3) Add a flocculation carrier to the fermentation broth, add 7.5g / 100mL fermentation broth, and then continue to ferment and culture for 2 days to obtain the raw material solution. The flocculation carrier is sandy soil, preferably the natural sandy soil for cultivating clam clams. Sterilize for use after sieving through a 250-mesh screen;

[0042] (4) Add ethanol to the raw material liquid and ...

Embodiment 3

[0045] (1) Inoculate the strain on a solid medium at 23°C for 48 hours, then add sterile water to make a strain liquid, the volume ratio of sterile water to solid medium is 1.5:1;

[0046] (2) Inoculate the bacterial seed liquid into the liquid culture medium, the inoculation concentration is 1.4mL bacterial seed liquid / 100mL liquid medium, ferment culture at 23℃ for 5 days, the shaker speed is 200r / min, make the fermentation bacterial liquid;

[0047] (3) Add a flocculation carrier to the fermentation broth, add 10g / 100mL fermentation broth, and then continue to ferment and culture for 3 days to obtain the raw material solution. The flocculation carrier is sandy soil, preferably natural sandy soil for cultivating clam clam philippines. Sterilize for use after sieving through the mesh screen;

[0048] (4) Add ethanol to the raw material liquid and let it settle. The volume ratio of ethanol to raw material liquid is 4:1. Let it stand at 5℃ for 10 hours, then centrifuge at 5000r / min to...

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Abstract

The invention relates to the technical field of water treatment, in particular to psychrobacter aquimaris and a method for preparing a flocculating agent by the psychrobacter aquimaris. The psychrobacter aquimaris Psychrobacter sp. GHF2 is separated and purified from sludge liquor spit by ruditapes philippinarum, a strain of the psychrobacter aquimaris is subjected to enlarging cultivation to prepare strain liquid, the strain liquid is fermented and cultivated to prepare fermented liquid, a flocculating carrier is added into the fermented liquid in a fermentation late period, and the flocculating carrier and added ethyl alcohol are mutually reinforced, so that extracellular polysaccharide in the fermented liquid is safely settled. The extracellular polysaccharide secreted by the screened psychrobacter aquimaris Psychrobacter sp. GHF2 has strong flocculating effects, the flocculating carrier is added in the fermentation late period, so that bacterial cells are clumped on the flocculating carrier, settling of the extracellular polysaccharide is promoted, use of the ethyl alcohol is reduced, and the obtained flocculating agent is strong in flocculating capacity and stable in performance.

Description

Technical field [0001] The invention relates to the technical field of water treatment, in particular to a strain of marine cryobacter and a method for preparing a flocculant using the strain. Background technique [0002] my country’s urbanization has developed rapidly in the past ten years, but due to the delayed investment in sewage treatment facilities, low sewage treatment efficiency, a large amount of industrial wastewater and domestic sewage discharge, serious water environment deterioration has caused heavy metals, pigments and suspended particles in the water to exceed the standard. Water resources have now become an important factor restricting the development of social urbanization and industrialization. Improving sewage treatment technology and strengthening urban sewage treatment capacity has become a top priority. Among them, the introduction of flocculent particles of appropriate size into the sewage, the use of its huge surface area to flocculate and adsorb heavy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/01
Inventor 穆军崔霞杨桥
Owner ZHEJIANG OCEAN UNIV
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