31 results about "Scapharca subcrenata" patented technology

The invention relates to multipiptide protein active substance in a kind of bloody clam (Arca subcrenata Lischke; Scapharca subcrenata), the prepn. method and the uses. The prepn. method is that bloody clam is homogenated to obtain superantant which is treated by chromatography in ion exchange column, desalt, chromatograph separation in gel colum to be combined to several peaks with molecular weight 3-15 KDa which is concentrated and dried to obtain multipeptide protein active substance. Said active substance has 73.72 percent of cancer-inhibiting ratio and can be made as anti-cancer medicine.

The invention relates to an method for extracting small molecule active polypeptide, consisting of shuck off the arca subcrenata shell, washing, homogenating, adding distilled water for a low temperature extraction, flocculating, centrifugation, removing the dregs, taking supernatant fluid, doing a gel column chromatography after freeze drying the supernatant fluid, collecting respective peak, then doing an ion-exchange chromatography to achieve the separated purification, collecting the active peak, desalting by gel column after freeze-dry to get a highly purified natural active polypeptide, going on purifying the produced small molecule peptide by reversed-phase column to get the active peptide with a purity more than 95%. The extraction progress effectively protects the activity of the peptide. The active peptide has an obvious anti-tumor activity with a tumor-inhibiting rate to 52% proved by anti-tumor experiments in vitro and can be used for the exploiture of new-type anti-tumor drug.

The invention relates to the technical field of aquaculture, in particular to an eco-aggregation type scapharca subcrenata spat breeding method. The method includes steps: taking a coastal aquaculture pond as a breeding water source, using microbial preparations for controlling water quality of the pond in a breeding process, and optimizing and reinforcing bait absorption and utilization; after larval metamorphosis, enabling larvae to directly attach to the bottom of the pond without adoption of a substratum, directly using water of the pond for breeding, feeding a small quantity of unit-cell alga baits such as platymonas for 3-5 days, stopping feeding, and enabling juveniles to be completely fed with unit-cell alga baits in water of the pond; when the juveniles grow to 3mm above, performing pond breeding or marine suspension aquaculture. By means of micro-ecology, antibiotic drugs are avoided in a whole spat breeding process, and accordingly food safety is guaranteed; nutrition optimization, natural conversion and comprehensive substitution of the artificially cultured unit-cell alga baits and the natural baits in the pond are realized; in addition, due to removal of the substratum, operation procedures are optimized.

The invention discloses a primer group and probe for detecting ostreid herpesvirus of infected scapharca subcrenata, and application of the primer group and probe. The primer group consists of a forward primer and a backward primer; the base sequence of the forward primer is as shown in SEQ ID NO. 1, and the base sequence of the backward primer is as shown in SEQ ID NO. 2. The base sequence of theprobe is as shown in SEQ ID NO. 3. The invention further discloses application of the primer group and the probe in preparation of a reagent for RPA detecting the ostreid herpesvirus of the infectedscapharca subcrenata. The reagent is short in virus detection time, high in yield and high in specificity; positive and negative results have an obvious difference, so that the verification rate is high, and the reagent is more obvious and reliable.

The invention discloses an environment-friendly stone imitating house biological decoration material, which is characterized by including the following substances by weight: 29-36 parts of a water-based elastic acrylic emulsion, 12-19 parts of carbamate, 3-6 parts of oak sawdust, 2-6 parts of Ailanthus altissima sawdust, 1-6 parts of Schima superba sawdust, 1-3 parts of stannous decanoate, 9 parts of polybutylene glycol, 11-13 parts of clam shell powder, 13-15 parts of Scapharca subcrenata shell powder, 14-16 parts of Mactra veneriformis shell powder, 4-8 parts of calcium stearate, 9-16 parts of lignocellulose, 6-7 parts of methyl benzoate, 9-14 parts of polyvinyl alcohol, 3-6 parts of chlorobenzene, and 6-13 parts of a dispersant. With excellent environmental protection performance, the decoration material provided by the invention has a stone imitating effect, and can meet the needs of market diversification, humanization, creativation and individuation.

The invention discloses a special biofertilizer for tea trees. The special biofertilizer contains the following substances in parts by weight: 15-20 parts of ammonium nitrate, 9-12 parts of potassium sulfate, 0.1-0.2 part of lactobacillus powder, 10-15 parts of highland barley straw powder, 30-45 parts of horse bean straw powder, 60-80 parts of wheat straw powder, 8-12 parts of scapharca subcrenata shell powder, 3-9 parts of surface clamshell powder, 3-5 parts of ginkgo wood sawdust, 3-6 parts of oak wood sawdust, 2-4 parts of potassium polymetaphosphate, 3-8 parts of potassium pyrophosphate, 4-8 parts of sodium aluminum phosphate, 0.1-0.2 part of nitrobacterium powder, 0.1-0.2 part of rhizobium powder, 3-6 parts of zinc sulfate and 2-5 parts of sepiolite. The special biofertilizer is reasonable in formula and balanced in nutrition, is safe and pollution-free and fully meets the short / long-term nutrient requirements of the tea trees, balanced fertilization can be achieved, the soil can be improved, the environment can be protected, the yield of tea can be effectively increased, and the quality of the tea can be effectively improved.

The invention relates to a method for using 300-mu zeolite powder as a Scapharca subcrenata and juvenile mollusk attachment base. The method comprises the following steps of: putting 8-15 cm of seawater in an empty tank with the depth of 1-1.7 m, putting 1.5-2 kg / m2 of the 300-mu zeolite powder, uniformly stirring, and discharging upper water after the zeolite powder precipitates; then, putting 30-35 cm of seawater, uniformly stirring and discharging upper water again after the zeolite powder precipitates; and finally, putting seawater till the tank is full, and putting Scapharca subcrenata larva to be attached after the zeolite powder precipitates. According to the method disclosed by the invention, the problems of being fussy in treatment process, high in cost, inconvenient for screening, non-strict for sterilization and resulted in diseases in the current Scapharca subcrenata and juvenile mollusk attachment base can be solved; the Scapharca subcrenata and juvenile mollusk attachment survival rate is increased; the attachment survival rate can be increased to 28-35%; the growth speed is increased; furthermore, the treatment method is simple; without sterilization, the attachmentbase can be prevented from bringing about harmful organisms; furthermore, the method has the functions of purifying water quality, adsorbing ammonia, nitrogen and bacteria and the like; and the effect is obvious.

The invention discloses a breeding method of scapharca subcrenata rapid growth varieties. The method includes the steps of base-population establishment, selectively-bred F1 generation construction, selective breeding effect evaluation and continuous selective breeding. The method specifically includes the steps that mature individuals of wild scapharca subcrenata of the same age are determined asbreeding base populations; the individuals with the shell length and the wet weight 8-10% of these of the base populations are selected, and a conventional method is adopted for cultivating the individuals into F1 generations; the shell length, the total weight and other characters of selectively-bred generations and the shell length, the total weight and other characters of control groups whichare not selectively bred and cultivated in the same period are measured periodically, the genetic parameter is estimated, and the genetic gain of the shell length and the total weight of each selectively-bred generation is about 3-6%. The method uses the shell length and the total weight as selective breeding objects, 8-10% high selection pressure is used for continuous selective breeding, and compared with the control groups, the cultivated new scapharca subcrenata varieties can show 3-6% growth advantages in the aspects of the shell length and the total weight. The excellent varieties cultivated by the method can serve as rapid growth varieties, can be directly used, and can be hybridized with other excellent varieties for breeding, and the scapharca subcrenata varieties having other excellent properties are selectively bred.

The invention discloses a marine bioactive extract-containing bacterium removing and formaldehyde removing adsorption material. The marine bioactive extract-containing bacterium removing and formaldehyde removing adsorption material is prepared from the following raw materials in parts by mass: 3 to 5 parts of a marine biological bacteria remover, 8 to 17 parts of crabshell powder, 5 to 9 parts ofcrithmum maritimum, 2 to 8 parts of red raspberry powder, 5 to 10 parts of dried tea residues, 1 to 3 parts of essence, 4 to 7 parts of spirulina powder, 1 to 2 parts of glycine betaine, 2 to 5 partsof glass micro-beads and 40 to 100 parts of deionized water; the marine biological bacteria remover is prepared from the following raw materials in parts by weight: 5 to 8 parts of hydrolyzed rhodophycea extract, 5 to 8 parts of steroidal saponin, 4 to 6 parts of scapharca subcrenata polypeptide, 1 to 3 parts of squid ink, and 3 to 6 parts of oligopeptide of marine fish. The adsorption material disclosed by the invention has excellent formaldehyde adsorption performance; the formaldehyde removal rate in 48 hours can reach 98 percent; a preparation method is simple and facilitates industrial production; the adsorption material is high in safety and protects human health.

The invention relates to multipiptide protein active substance in a kind of bloody clam (Arca subcrenata Lischke; Scapharca subcrenata), the prepn. method and the uses. The prepn. method is that bloody clam is homogenated to obtain superantant which is treated by chromatography in ion exchange column, desalt, chromatograph separation in gel colum to be combined to several peaks with molecular weight 3-15 KDa which is concentrated and dried to obtain multipeptide protein active substance. Said active substance has 73.72 percent of cancer-inhibiting ratio and can be made as anti-cancer medicine.

The invention discloses a biofertilizer for tea trees. The biofertilizer is characterized by containing the following substances in parts by weight: 25-30 parts of potato straw powder, 30-40 parts of highland barley straw powder, 15-20 parts of horse bean straw powder, 3-8 parts of potassium pyrophosphate, 0.001-0.003 part of aschersonia powder, 10-15 parts of highland barley straw powder, 30-45 parts of horse bean straw powder, 60-80 parts of wheat straw powder, 8-12 parts of scapharca subcrenata shell powder, 3-9 parts of surface clamshell powder, 3-5 parts of ginkgo wood sawdust, 3-6 parts of oak wood sawdust, 2-4 parts of potassium polymetaphosphate and 0.001-0.003 part of bacillus thuringiensis powder. The biofertilizer has the beneficial effects that the fertilizer disclosed by the invention is rich in a variety of nutrients, such as organic fertilizers, inorganic fertilizers and the like, is free from toxicity, side effects, residues and drug resistance and does not pollute the environment, so that the growth of tea is facilitated, nutritional ingredients required by the tea can be supplemented obviously, the quality of the tea is improved, the content of substances, beneficial to human bodies, in the tea is increased, and the soil can be improved.

The invention discloses an environment-friendly material of an energy-saving lamp. The environment-friendly material is characterized by comprising following materials in parts by weight: 19-36 parts of polyesters, 20-30 parts of gypsum powder, 10-15 parts of silicate cement, 1-2 parts of an antistatic agent, 9-16 parts of plastified starch, 12-16 parts of scapharca subcrenata shell powder, 9-15 parts of oyster shell powder, 3-6 parts of clam shell powder, 6-10 parts of hollow glass micro-beads, 3-6 parts of nitrobenzene and 3-6 parts of porous-closed micro-beads. With the adoption of the material, a lamp holder of the energy-saving lamp keeps the good performance under a high temperature and long time; the environment-friendly material can be used for a long period and can be widely applied to illumination of public places.

The invention discloses a glass thermal insulation bio-coating. The glass thermal insulation bio-coating is characterized by comprising the following substances in parts by weight: 20-30 parts of silane coupling agent, 17-20 parts of scapharca subcrenata shell powder, 16-25 parts of mactra veneriformis shell powder, 11-16 parts of oak sawdust, 20-23 parts of willow sawdust, 3-6 parts of laminated structure mica powder, 1-3 parts of hollow glass microball, 2-8 parts of quartz sand, 1-3 parts of crylic acid, 4-6 parts of methyl fiber, 6-8 parts of fire retardant, 3-9 parts of organic silicone modified epoxy resin, 1-3 parts of mixed solvent, 2-3 parts of inorganic thickener, 14-25 parts of coalescing agent, and 4-9 parts of cosolvent. By adopting the glass thermal insulation bio-coating, the defects of excessive level of a volatilizing organic solvent, uneven size distribution, unstable performance, large energy consumption for production and environment pollution caused by dispersing metallic oxide nano-powder by an organic solvent or high power consumption grinding are overcome; and the glass thermal insulation bio-coating has excellent optical performance, mechanical performance and chemical resistance.

The invention discloses a making technology of ready-to-eat seafood poultry egg custard. The making technology comprises the following steps of beating poultry egg shells, removing the poultry egg shells, and performing stirring so as to obtain egg fluid; cutting up meat of marine products into meat dices of 0.4-1cm; uniformly mixing the egg fluid with the broken meat of the marine products in the ratio of the egg fluid to the broken meat of the marine products being 3 to 2 so as to obtain a mixture; adding condiments, wherein table vinegar accounts for 0.4-1% of the weight of the mixture, table salt accounts for 0.4-2% of the weight of the mixture, and gourmet powder accounts for 0.3-1% of the weight of the mixture; and treating the mixture, the table vinegar, the table salt and the gourmet powder for 13-18 minutes under the pressure of 600-650MPa so as to obtain finished products, and loading the finished products into bags. According to the making technology disclosed by the invention, poultry eggs can include the kinds of chicken eggs, duck eggs, goose eggs or quail eggs; the broken meat of the aquatic products can include the varieties of mantis shrimps, scapharca subcrenata, scallops, shrimps, fishes or trepangs; the ready-to-eat seafood poultry egg custard is viscous fluid with the color of the egg fluid, has the characteristics of being novel in forms, unique in taste and convenient to carry, also has the advantages of being low in energy consumption, low in cost and suitable for industrialized production, and can be eaten by people at hotels and families, and tourists during travelling.

The invention relates to a bottom sowing method for Scapharca subcrenata juveniles. The method selects the muddy sand seabed with water depth between 4 and 6 meters and water temperature between 10 and 18 DEG C; the Scapharca subcrenata juveniles are put into the muddy sand according to the ratio that mud is between 60 and 80 percent, and sand is between 40 and 20 percent seabed; and the bottom density is between 30 and 50 particles / m<2>. The method solves the problem of exhaustion of resources caused by random fishing when Scapharca subcrenatas grow in natural environment, can ensure that the Scapharca subcrenata juveniles grow quickly in natural environment and meet commercial specification within two years, so that wide consumers can enjoy the flavor of the Scapharca subcrenata.

The invention discloses a medicine for treating the tourette syndrome of children. The medicine comprises, by weight, 8-17 parts of humifuse euphorbia herb, 4-15 parts of cuttlebone, 5-11 parts of scapharca subcrenata meat, 4-8 parts of crneate lespedeza, 4-9 parts of fargesii, 5-8 parts of radix dipsaci, 5-10 parts of siraitia grosvenorii, 4-7 parts of merremia yunnanensis, 0.1-1 part of pomegranate bark, 1-3 parts of jasmine flowers, 2-5 parts of spina date seeds, 3-7 parts of tribulus terrestris, 3-8 parts of platycodon grandiflorum, 2-5 parts of codonopsis pilosula, 0.1-1 part of roasted turtle shells and 1-3 parts of mulberries. The medicine can effectively treat the tourette syndrome of children, the effective rate reaches 98%, the raw materials are easy to obtain, the toxicity is low, and the medicine is free of side effects, safer and beneficial to protecting health of children.

The invention discloses an energy-saving wall coating material, which is characterized by including the following substances by weight: 3-9 parts of sepiolite, 2-3 parts of perlite, 1-6 parts of diatomite, 1-2 parts of zinc powder, 2-5 parts of lead oxide, 1-2 parts of zirconia, 15-19 parts of silica, 2-9 parts of hydrogen peroxide, 2-9 parts of polypropylene, 20-35 parts of polytetrafluoroethylene gum, 15-20 parts of alkyd resin, 20-28 parts of melamine resin, 9-16 parts of Scapharca subcrenata shell powder, 14-18 parts of conch shell powder, 5-9 parts of light plastering gypsum, 1-3 parts of perlite, and 10-20 parts of high alumina cement. The energy-saving wall coating material provided by the invention has the advantages of fast wet-dry curing, high bonding strength, water and moisture resistance. After using the wall coating material provided by the invention, the wall is free of chalking, cracking, hollowing, shedding, frosting and other phenomena.

The invention discloses a lightweight building material which is characterized by comprising the following substances, by weight, 50-80 parts of cement, 5-9 parts of a mixed solvent, 6-8 parts of an inorganic thickening agent, 11-18 parts of clam shell powder, 2-8 parts of Scapharca subcrenata shell powder, 9-17 parts of white clamshell powder, 1-6 parts of polypropylene, 3-8 parts of nano-calcium sulfate, 3-9 parts of a titanate coupling agent, 2-8 parts of abietyl resin acid, 20-32 parts of organosilicone modified epoxy resin, 1-9 parts of laminated structured mica powder, 1-3 parts of hollow glass beads, 1-6 parts of linear low density polyethylene, 2-3 parts of white oil, 3-9 parts of fiber and 3-9 parts of a foaming agent. By the use of composite cement and lime, slurry thickening time can be shortened, and bubbles coming from the foaming agent and linear low density polyethylene can be fully maintained so as to guarantee thermal insulation property of the material; and by simultaneous application of two foaming agents, namely physical and chemical foaming agents, it helps to raise thermal insulation performance of the slurry.

The invention relates to a scapharca subcrenata-derived calcium binding protein ASP-3 with antitumor activity and a structure thereof, and belongs to the technical field of biology. The recombinant calcium binding protein contains 200 amino acids, and the total length of the corresponding coding gene nucleotide is 603bp. The protein acts on an EGFR target of HepG2 tumor cells to inhibit tumor growth. The scapharca subcrenata calcium binding protein can be used in the medicine and health care product industry, and can be further used for research of liver cancer molecular targeted therapeutic drugs, marine antitumor protein resources can be fully utilized, and considerable economic benefits can be generated by obtaining rich antitumor proteins.

The invention provides an extraction method of active peptides of scapharca subcrenata. The method comprises the steps as follows: scapharca subcrenata is shelled, cleaned, homogenized, extracted at a low temperature with distilled water, flocculated and centrifuged for slag removal; a supernate is taken, lyophilized and subjected to gel column chromatography, peaks are collected, ion exchange column chromatography is performed for separation and purification, active peaks are collected, lyophilized and desalted with a gel column, natural active peptides with higher purity are obtained, obtained small-molecular peptides are purified by a C18 reverse phase column, and the active peptides with purity being 95% or higher are obtained. The molecular weight of the scapharca subcrenata small-molecular peptides obtained with the method is 500-3000 Da, and the biological activity of the small-molecular peptides is effectively protected in the extraction process. The active peptides have significant anti-tumor activity, in-vitro anti-tumor experiments show that the tumor inhibition rate reaches 52%, and the active peptides can be used for the development and application of novel anti-tumor drugs.

The invention relates to a method for using 300-mu zeolite powder as a Scapharca subcrenata and juvenile mollusk attachment base. The method comprises the following steps of: putting 8-15 cm of seawater in an empty tank with the depth of 1-1.7 m, putting 1.5-2 kg / m2 of the 300-mu zeolite powder, uniformly stirring, and discharging upper water after the zeolite powder precipitates; then, putting 30-35 cm of seawater, uniformly stirring and discharging upper water again after the zeolite powder precipitates; and finally, putting seawater till the tank is full, and putting Scapharca subcrenata larva to be attached after the zeolite powder precipitates. According to the method disclosed by the invention, the problems of being fussy in treatment process, high in cost, inconvenient for screening, non-strict for sterilization and resulted in diseases in the current Scapharca subcrenata and juvenile mollusk attachment base can be solved; the Scapharca subcrenata and juvenile mollusk attachment survival rate is increased; the attachment survival rate can be increased to 28-35%; the growth speed is increased; furthermore, the treatment method is simple; without sterilization, the attachmentbase can be prevented from bringing about harmful organisms; furthermore, the method has the functions of purifying water quality, adsorbing ammonia, nitrogen and bacteria and the like; and the effect is obvious.

The invention discloses a method for extracting active peptide from scapharca subcrenata. The method comprises the following specific steps that the fresh scapharca subcrenata is collected, subjectedto shell removal and added into distilled water, and through homogenizing processing, soaking and filtration, filtration liquid A is obtained; an acetic acid solution is added into the filtration liquid A, an electric rod is adopted for conducting stirring and extraction, through centrifugal processing, a supernate is collected, NaCl is added into the supernate until the final concentration of themixed solution is 2.5 mol / L, a flocculent precipitate is separated out and centrifuged, and the scapharca subcrenata active peptide is obtained. According to the method, the extracted scapharca subcrenata active peptide is high in activity and extraction rate and contains high-content hydrophobic amino acid.

The invention discloses an inflaming retarding building coating material. The inflaming retarding building coating material is characterized by comprising the following substances in parts by weight: 20-30 parts of expanded perlite, 10-25 parts of a mineral heat-insulation material, 11-26 parts of glass fibers, 9-14 parts of scallop shell powder, 9-14 parts of scapharca subcrenata shell powder, 11-15 parts of oyster shell powder, 8-12 parts of stearic amide, 8-16 parts of machine-made sand, 15-23 parts of polyvinyl butyral, 20-26 parts of PA66 resin, 4-8 parts of oleamide, 5-9 parts of tin tetrachloride, 10-27 parts of iron oxide and 100 parts of water. The inflaming retarding building coating material has the effects of light weight, high strength, strong binding power, no seam, inflaming retarding performance, no hollowing, no cracks, no pulverization, non-combustible performance, no expansion, waterproof performance, strong weather resistance, good heat-preservation and heat-insulation performance, impervious and anti-cracking performance, strong flexibility and good integrality.

The invention discloses biological organic fertilizer and a preparation method thereof. The biological organic fertilizer is organic food tea plantation special-purpose fertilizer and comprises, by weight, 35-45 parts of urea, 15-20 parts of ammonium dihydrogen phosphate, 16-26 parts of potassium dihydrogen phosphate, 9-14 parts of kaolin, 8-15 parts of bauxite, 3-6 parts of potassium tripolyphosphate, 2-6 parts of sodium metaphosphate, 7-14 parts of disodium hydrogen phosphate, 0.001-0.002 parts of bacillus thuringiensis powder, 25-30 parts of soybean straw powder, 25-30 parts of potato straw powder, 10-15 parts of scapharca subcrenata shell powder, 15-20 parts of Biaoha shell powder, 5-9 parts of China fir wood chip, 5-9 parts of platycladus orientalis wood chip, 0.001-0.002 parts of microzyme powder and 0.001-0.002 parts of actinomyces powder. Compared with the existing organic tea fertilization formula, the biological organic fertilizer contains macroelement nutrients such as N, P and K, contains a certain amount of medium and trace elements, and has good specificity by combination with a tea fertilizer needing rule and tea tree soil nutrient present situation.

The invention discloses a paste for relaxing muscles and tendons. The paste is characterized by comprising drug powder and drug flour; wherein the drug powder is composed of the following raw materials by weight: 90 grams of sinapis alba, 120 grams of cannabis sativa, 50 grams of euphorbia kansui, 105 grams of clematis root, 135 grams of garden balsam seed, 60 grams of carbonized hair, 150 grams of dark plum, 105 grams of phryma leptostachya, 90 grams of earthworm, 60 grams of scorpion, 60 grams of menispermaceae, 60 grams of unprocessed radix aconiti kusnezoffii, 60 grams of asarum herb, 60 grams of pangolin, 90 grams of oyster, 60 grams of scapharca subcrenata, 60 grams of dandelion, 50 grams of buck grass, 60 grams of erodium stephanianum willd, 60 grams of motherwort, 60 grams of cowherb seed, and 30 grams of corydalis bungeana; and the drug flour is prepared from the following components by weight: 3 grams of musk, 30 grams of frankincense, 30 grams of myrrh, 30 grams of dragon's blood, and 75 grams of borneol, and is prepared by grinding the weighed musk, frankincense, myrrh, dragon's blood, and borneol.

The invention discloses an energy-saving and high temperature resistant material, which is characterized by including the following substances by weight: 3-8 parts of ethyl orthosilicate, 2-6 parts of a mineral thermal insulation material, 1-6 parts of razor clam shell powder, 9-15 parts of razor clam king shell powder, 3-8 parts of scallop shell powder, 2-6 parts of Scapharca subcrenata shell powder, 3-7 parts of poly-4-methyl-1-pentene, 9-15 parts of fir sawdust, 3-6 parts of Platycladus orientalis sawdust, 8-14 parts of Ailanthus altissima sawdust, 1-6 parts of polyolefin plastic, 9-15 parts of alcohol soluble resin, 3-6 parts of phenolic aldehyde, 10-12 parts of alcohol soluble resin, 13-16 parts of fluorine resin, and 3-9 parts of inorganic filler. The energy-saving and high temperature resistant material provided by the invention takes kiln furniture waste as aggregate and a composite substrate as the main component, and has important significance to comprehensive utilization of waste resources and improvement of ecological environment.

The invention relates to a method for spawning, hatching and incubating scapharca subcrenata larvae in seawater disinfected by a bleaching solution. The method comprises the following steps of: under the condition that the water quality of the seawater for the seedling raising of scapharca subcrenata is blackened or a water surface has a large amount of foam, pumping the seawater to an indoor seedling raising pond, adding the bleaching solution, so that available chlorine in the pond is between 20 and 30 ppm, and precipitating for 48 to 72 hours; after precipitating, pumping the seawater to the other seedling raising pond, neutralizing residual chlorine by using sodium thiosulfate, and testing by using chlorine measuring test paper until the residual chlorine in the water is removed; introducing air into the seedling raising pond, putting unicellular algae in the addition quantity of between 0.02 and 0.03 million / milliliter; and after the water surface has white superfine air bubbles, putting breeding shellfish into the seedling raising pond in the addition quantity of 6 to 8 shellfish / m<3>, so that the breeding shellfish spawn and hatch, and breeding the hatched shellfish in the disinfected seawater after hatching. By the method, the problems that spawning and incubation of the scapharca subcrenata and the culture of the larvae are unstable are solved when the bacterium content of the seawater exceeds the standard; and the scapharca subcrenata can be grown to the length of 1,000 micrometers within 41 to 45 days, and the survival rate is between 30 and 35 percent.

The invention relates to the technical field of aquaculture, in particular to an eco-aggregation type scapharca subcrenata spat breeding method. The method includes steps: taking a coastal aquaculture pond as a breeding water source, using microbial preparations for controlling water quality of the pond in a breeding process, and optimizing and reinforcing bait absorption and utilization; after larval metamorphosis, enabling larvae to directly attach to the bottom of the pond without adoption of a substratum, directly using water of the pond for breeding, feeding a small quantity of unit-cell alga baits such as platymonas for 3-5 days, stopping feeding, and enabling juveniles to be completely fed with unit-cell alga baits in water of the pond; when the juveniles grow to 3mm above, performing pond breeding or marine suspension aquaculture. By means of micro-ecology, antibiotic drugs are avoided in a whole spat breeding process, and accordingly food safety is guaranteed; nutrition optimization, natural conversion and comprehensive substitution of the artificially cultured unit-cell alga baits and the natural baits in the pond are realized; in addition, due to removal of the substratum, operation procedures are optimized.

The invention discloses a scapharca subcrenata catalase gene. The scapharca subcrenata catalase gene is a clone gene which is prepared by cloning a core segment of the scapharca subcrenata catalase gene by using a gene cloning technology. The invention further discloses a novel marine biological pollution detection marker. The marine biological pollution detection marker is characterized in that scapharca subcrenata is taken as a biological sample for detecting marine pollution; the scapharca subcrenata catalase is taken as a detection marker for the marine pollution; the relative expression quantity of the scapharca subcrenata catalase gene is the highest in blood corpuscle and cheekbones of the scapharca subcrenata; a PCR (Polymerase Chain Reaction) reaction system is adopted in order toensure the specificity of an amplification reaction, and increase PCR amplification efficiency. The detection marker provided by the invention has high specificity, a sensitive biological reaction isperformed at a reaction endpoint by directly using a biological in vivo target cell or a target molecule at the molecular level, and exposure and toxicity effect of marine pollutant can be alarmed.